BBa_K1510603 1 BBa_K1510603 Dispersin generator ,with constitutive promoter plus yebf 2014-10-07T11:00:00Z 2015-05-08T01:10:48Z The promoter, J23100, comes from the 2014 distributed igem kit. The signal sequence, yebF, encoding signal protein exporting recombinant protein extracellular, is from E.coli K-12. By designing primer, we obtain it together with its naturally occurring ribosomal binding site using PCR and extraction. Finally, the dispersin is from Lyon INSA igem team. The Dispersin gene codes for an enzyme which catalyzes the hydrolysis of the extracellular matrix produced by Gram negative bacteria.With strong constitutive promoter,together with ribosomal binding site and signal sequence yebF, make dispersin work more accurately. false false _1890_ 0 21271 9 It's complicated false To fit igem part standard, the circuit is constructed in backbone pSB1C3, chloramphenicol with EcoR1 and Xba1 in front of the part, and Spe1, Pst1 behind the part. false Kao Jung Chang component2404685 1 BBa_K802001 component2404676 1 BBa_J23100 component2404677 1 BBa_K1510009 annotation2404685 1 BBa_K802001 range2404685 1 593 1916 annotation2404676 1 BBa_J23100 range2404676 1 1 35 annotation2404677 1 BBa_K1510009 range2404677 1 44 584 BBa_K802001 1 BBa_K802001 Dispersin generator for <i>B. subtilis</i> 2012-09-19T11:00:00Z 2016-02-12T09:05:08Z Dispersin is produced by Aggregatibacter actinomycetemcomitans. The part contains the DspB gene coding for an enzyme which catalyzes the hydrolysis of the extracellular matrix produced by Gram-negative bacteria. false false _1058_ 4206 13266 9 It's complicated true Used a strong RBS in order to produce high levels of protein. false Remi Hocq, Audrey Masi annotation2188639 1 Sigma A-35 range2188639 1 63 68 annotation2203894 1 BBa_B0015 range2203894 1 1212 1324 annotation2188643 1 BBa_K143021 range2188643 1 106 117 annotation2188641 1 BBa_K143012 range2188641 1 1 97 annotation2188644 1 BBa_K391006 range2188644 1 126 1211 annotation2188642 1 rbs range2188642 1 106 117 annotation2188640 1 Sigma A -10 range2188640 1 86 91 BBa_K1510009 1 BBa_K1510009 E.coli Signal sequence YebF 2014-10-05T11:00:00Z 2015-05-08T01:10:48Z The promoter, J23100, comes from the 2014 distributed igem kit. The signal sequence, yebF, encoding signal protein exporting recombinant protein extracellular, is from E.coli K-12. By designing primer, we obtain it together with its naturally occurring ribosomal binding site using PCR and extraction. Finally, the terminator B0015 is from 2014 igem distribution. This part is composed with a strong promoter, together with ribosomal binding site and signal sequence yebF, finally a double terminator. As a signal protein, YebF would be secreted outside E.coli. The role of the circuit in our project is basically a control circuit. By contrasting the result of this circuit and the other circuit, containing functional protein, we can assume if the protein works more accurately. false false _1890_ 0 21268 9 It's complicated false To fit igem part standard, the circuit is constructed in backbone pSB1C3, chloramphenicol with EcoR1 and Xba1 in front of the part, and Spe1, Pst1 behind the part. false Chien-Yung Huang BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_K802001_sequence 1 aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgtaaaggtggtgaaatgaaaaaagcaattactttatttacattattatgcgcggtacttctctcattcagtaccgcaacttatgcaaacgctatggacttacctaaaaaagaaagcggtctgacgttagatatcgcacgtcgtttctataccgttgatacgataaaacaatttatcgatacgattcatcaggcgggcggcacttttctgcatttacatttttccgatcacgagaattatgcattggaaagttcttatttggaacaacgagaagaaaatgcgaccgagaaaaacggaacctatttcaatccgaaaacaaataagccgtttctcacttataaacagctcaatgaaattatctattatgccaaagaacgaaatattgaaattgtgcctgaagtcgatagcccgaatcatatgacggcgatttttgatcttttaacccttaagcacggaaaggaatacgtaaaagggctaaaatcgccttatatcgccgaggaaatcgatattaataaccccgaagcggttgaagttattaaaaccttaatcggtgaagtgatctatattttcggacattcaagccggcatttccatatcggcggagatgaatttagctatgcggtcgaaaataatcatgaatttattcggtatgtgaataccttaaatgattttatcaattccaaagggctaattacccgtgtttggaatgacggtttgatcaaaaacaacttaagcgaactcaataaaaacattgaaatcacttactggagctacgacggtgacgctcaagccaaagaagatattcaatatcgacgtgaaataagagccgatttgcccgaactgctggcaaacggttttaaggttttaaactataattcttattatttatactttgtgcctaaatccggttctaatattcacaatgacggtaaatatgcggctgaagacgtattaaataactggacattaggtaaatgggacggaaaaaacagctcaaatcacgtacaaaatacgcagaatattatcggttcttctttgtcgatttggggggaacgttccagcgcattaaatgaacaaactattcagcaagcctctaaaaatttattaaaagcggtgatccaaaaaactaatgatccgaaatcgcattagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct BBa_K1510603_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagttgacggctagctcagtcctaggtacagtgctagctactagggagaaaaacatgaaaaaaagaggggcgtttttagggctgttgttggtttctgcctgcgcatcagttttcgctgccaataatgaaaccagcaagtcggtcactttcccaaagtgtgaagatctggatgctgccggaattgccgcgagcgtaaaacgtgattatcaacaaaatcgcgtggcgcgttgggcagatgatcaaaaaattgtcggtcaggccgatcccgtggcttgggtcagtttgcaggacattcagggtaaagatgataaatggtcagtaccgctaaccgtgcgtggtaaaagtgccgatattcattaccaggtcagcgtggactgcaaagcgggaatggcggaatatcagcggcgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgtaaaggtggtgaaatgaaaaaagcaattactttatttacattattatgcgcggtacttctctcattcagtaccgcaacttatgcaaacgctatggacttacctaaaaaagaaagcggtctgacgttagatatcgcacgtcgtttctataccgttgatacgataaaacaatttatcgatacgattcatcaggcgggcggcacttttctgcatttacatttttccgatcacgagaattatgcattggaaagttcttatttggaacaacgagaagaaaatgcgaccgagaaaaacggaacctatttcaatccgaaaacaaataagccgtttctcacttataaacagctcaatgaaattatctattatgccaaagaacgaaatattgaaattgtgcctgaagtcgatagcccgaatcatatgacggcgatttttgatcttttaacccttaagcacggaaaggaatacgtaaaagggctaaaatcgccttatatcgccgaggaaatcgatattaataaccccgaagcggttgaagttattaaaaccttaatcggtgaagtgatctatattttcggacattcaagccggcatttccatatcggcggagatgaatttagctatgcggtcgaaaataatcatgaatttattcggtatgtgaataccttaaatgattttatcaattccaaagggctaattacccgtgtttggaatgacggtttgatcaaaaacaacttaagcgaactcaataaaaacattgaaatcacttactggagctacgacggtgacgctcaagccaaagaagatattcaatatcgacgtgaaataagagccgatttgcccgaactgctggcaaacggttttaaggttttaaactataattcttattatttatactttgtgcctaaatccggttctaatattcacaatgacggtaaatatgcggctgaagacgtattaaataactggacattaggtaaatgggacggaaaaaacagctcaaatcacgtacaaaatacgcagaatattatcggttcttctttgtcgatttggggggaacgttccagcgcattaaatgaacaaactattcagcaagcctctaaaaatttattaaaagcggtgatccaaaaaactaatgatccgaaatcgcattagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct BBa_K1510009_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagggagaaaaacatgaaaaaaagaggggcgtttttagggctgttgttggtttctgcctgcgcatcagttttcgctgccaataatgaaaccagcaagtcggtcactttcccaaagtgtgaagatctggatgctgccggaattgccgcgagcgtaaaacgtgattatcaacaaaatcgcgtggcgcgttgggcagatgatcaaaaaattgtcggtcaggccgatcccgtggcttgggtcagtttgcaggacattcagggtaaagatgataaatggtcagtaccgctaaccgtgcgtggtaaaagtgccgatattcattaccaggtcagcgtggactgcaaagcgggaatggcggaatatcagcggcgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z