LOCUS       BBa_K1510915            1985 bp    DNA     linear       28-Jun-2024
DEFINITION  ketoisocaproate decarboxylase (THI3) Circuit
ACCESSION   BBa_K1510915
VERSION     BBa_K1510915.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    19..1848
                     /label=BBa_K1510087
     oriT            1..1848
                     /label=BBa_K1510922
     stem_loop       1868..1911
                     /label=stem_loop
     misc_feature    1857..1936
                     /label=BBa_B0010
     terminator      1857..1936
                     /label=BBa_B0010
     stem_loop       1952..1971
                     /label=T7 TE
     misc_feature    1978..1978
                     /label=stop
     polyA_site      1972..1985
                     /label=polya
     misc_feature    1945..1985
                     /label=BBa_B0012
     terminator      1945..1985
                     /label=BBa_B0012
     terminator      1857..1985
                     /label=BBa_B0015
ORIGIN
        1 aaagaggaga aatactagat gaattctagc tatacacaga gatatgcact gccgaagtgt
       61 atagcaatat cagattatct tttccatcgg ctcaaccagc tgaacataca taccatattt
      121 ggactctccg gagaatttag catgccgttg ctggataaac tatacaacat tccgaactta
      181 cgatgggccg gtaattctaa tgagttaaat gctgcctacg cagcagatgg atactcacga
      241 ctaaaaggct tgggatgtct cataacaacc tttggtgtag gcgaattatc ggcaatcaat
      301 ggcgtggccg gatcttacgc tgaacatgta ggaatacttc acatagtggg tatgccgcca
      361 acaagtgcac aaacgaaaca actactactg catcatactc tgggcaatgg tgatttcacg
      421 gtatttcata gaatagccag tgatgtagca tgctatacaa cattgattat tgactctgaa
      481 ttatgtgccg acgaagtcga taagtgcatc aaaaaggctt ggatagaaca gaggccagta
      541 tacatgggca tgcctgtcaa ccaggtaaat ctcccgattg aatcagcaag gcttaataca
      601 cctctggatt tacaattgca taaaaacgac ccagacgtag agaaagaagt tatttctcga
      661 atattgagtt ttatatacaa aagccagaat ccggcaatca tcgtagatgc atgtactcgt
      721 cgacagaatt taatcgagga gactaaagag ctttgtaata ggcttaaatt tccagttttt
      781 gttacaccta tgggtaaggg tacagtaaac gaaacagacc cgcaatttgg gggcgtattc
      841 acgggctcga tatcagcccc agaagtaaga gaagtagttg attttgccga ttttatcatc
      901 gtcattggtt gcatgctctc cgaattcagc acgtcaactt tccacttcca atataaaact
      961 aagaattgtg cgctactata ttctacatct gtgaaattga aaaatgccac atatcctgac
     1021 ttgagcatta aattactact acagaaaata ttagcaaatc ttgatgaatc taaactgtct
     1081 taccaaccaa gcgaacaacc cagtatgatg gttccaagac cttacccagc aggaaatgtc
     1141 ctcttgagac aagaatgggt ctggaatgaa atatcccatt ggttccaacc aggtgacata
     1201 atcataacag aaactggtgc ttctgcattt ggagttaacc agaccagatt tccggtaaat
     1261 acactaggta tttcgcaagc tctttgggga tctgtcggat atacaatggg ggcgtgtctt
     1321 ggggcagaat ttgctgttca agagataaac aaggataaat tccccgcaac taaacataga
     1381 gttattctgt ttatgggtga cggtgctttc caattgacag ttcaagaatt atccacaatt
     1441 gttaagtggg gattgacacc ttatattttt gtgatgaata accaaggtta ctctgtggac
     1501 aggtttttgc atcacaggtc agatgctagt tattacgata tccaaccttg gaactacttg
     1561 ggattattgc gagtatttgg ttgcacgaac tacgaaacga aaaaaattat tactgttgga
     1621 gaattcagat ccatgatcag tgacccaaac tttgcgacca atgacaaaat tcggatgata
     1681 gagattatgc taccaccaag ggatgttcca caggctctgc ttgacaggtg ggtggtagaa
     1741 aaagaacaga gcaaacaagt gcaagaggag aacgaaaatt ctagcgcagt aaatacgcca
     1801 actccagaat tccaaccact tctaaaaaaa aatcaagttg gatactgata ctagagccag
     1861 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1921 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1981 ttata
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