##gff-version 3
##sequence-region BBa_K1513000 1 1716
BBa_K1513000	.	point_mutation	237	237	.	+	0	ID=annotation2397489;Name=T
BBa_K1513000	.	CDS	64	813	.	+	0	ID=annotation2397486;Name=luxR
BBa_K1513000	.	non_covalent_binding_site	990	1009	.	+	0	ID=annotation2397498;Name=LuxR/HSL
BBa_K1513000	.	engineered_tag	820	844	.	+	0	ID=annotation2397491;Name=Help:Barcodes
BBa_K1513000	.	promoter	1009	1014	.	+	0	ID=annotation2397500;Name=-35
BBa_K1513000	.	engineered_tag	1692	1716	.	+	0	ID=annotation2397510;Name=Help:Barcodes
BBa_K1513000	.	stop_codon	974	974	.	+	0	ID=annotation2397497;Name=stop
BBa_K1513000	.	engineered_region	1074	1691	.	+	0	ID=annotation2397507;Name=BBa_C0061
BBa_K1513000	.	start_codon	1042	1042	.	+	0	ID=annotation2397502;Name=start
BBa_K1513000	.	ribosome_entry_site	1053	1067	.	+	0	ID=annotation2397505;Name=RBS-1\Strong
BBa_K1513000	.	engineered_region	46	57	.	+	0	ID=annotation2397484;Name=BBa_B0034
BBa_K1513000	.	operator	1	37	.	+	0	ID=annotation2397479;Name=P(Lac) IQ
BBa_K1513000	.	point_mutation	555	555	.	+	0	ID=annotation2397490;Name=A
BBa_K1513000	.	promoter	3	8	.	+	0	ID=annotation2397481;Name=-35
BBa_K1513000	.	engineered_region	64	65	.	+	0	ID=annotation2397487;Name=prefix
BBa_K1513000	.	stem_loop	864	907	.	+	0	ID=annotation2397493;Name=stem_loop
BBa_K1513000	.	promoter	1031	1036	.	+	0	ID=annotation2397501;Name=-10
BBa_K1513000	.	engineered_region	1053	1067	.	+	0	ID=annotation2397504;Name=BBa_B0030
BBa_K1513000	.	engineered_region	46	981	.	+	0	ID=annotation2397483;Name=BBa_I0462
BBa_K1513000	.	engineered_region	853	932	.	+	0	ID=annotation2397492;Name=BBa_B0010
BBa_K1513000	.	polyA_site	968	981	.	+	0	ID=annotation2397496;Name=polya
BBa_K1513000	.	engineered_region	1053	1716	.	+	0	ID=annotation2397511;Name=BBa_K081008
BBa_K1513000	.	engineered_region	64	819	.	+	0	ID=annotation2397488;Name=BBa_C0062
BBa_K1513000	.	conserved_region	50	53	.	+	0	ID=annotation2397485;Name=conserved
BBa_K1513000	.	stem_loop	948	967	.	+	0	ID=annotation2397495;Name=T7 TE
BBa_K1513000	.	engineered_region	1	37	.	+	0	ID=annotation2397480;Name=BBa_I14032
BBa_K1513000	.	engineered_region	1	1044	.	+	0	ID=annotation2397503;Name=BBa_J09855
BBa_K1513000	.	engineered_region	941	981	.	+	0	ID=annotation2397494;Name=BBa_B0012
BBa_K1513000	.	engineered_region	990	1044	.	+	0	ID=annotation2397499;Name=BBa_R0062
BBa_K1513000	.	tag	1653	1684	.	+	0	ID=annotation2397509;Name=LVA
BBa_K1513000	.	ribosome_entry_site	1060	1064	.	+	0	ID=annotation2397506;Name=RBS
BBa_K1513000	.	promoter	26	31	.	+	0	ID=annotation2397482;Name=-10
BBa_K1513000	.	CDS	1074	1652	.	+	0	ID=annotation2397508;Name=luxI
>BBa_K1513000
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaacataaatg
ccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatat
gactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatc
ctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattatt
ctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaa
agaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagt
tttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttcctt
ctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatg
tttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttc
catttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaa
ttgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtt
tgttatagtcgaataaatactagagattaaagaggagaaatactagatgactataatgataaaaaaatcggattttttg
gcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttag
ttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaa
tgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaa
cagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataact
ctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaac
agtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcat
gtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaa
acgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc