LOCUS BBa_K1513001 3144 bp DNA linear 23-Dec-2024 DEFINITION Fast-type merR biosensor ACCESSION BBa_K1513001 VERSION BBa_K1513001.1 FEATURES Location/Qualifiers RBS 1053..1067 /label=RBS-1\Strong RBS 1060..1064 /label=RBS misc_feature 1053..1067 /label=BBa_B0030 RBS 1053..1067 /label=BBa_B0030 misc_feature 1..37 /label=P(Lac) IQ misc_feature 1..37 /label=BBa_I14032 promoter 3..8 /label=-35 promoter 26..31 /label=-10 misc_feature 46..981 /label=BBa_I0462 misc_feature 46..57 /label=BBa_B0034 misc_feature 50..53 /label=conserved CDS 64..813 /label=luxR misc_feature 64..65 /label=prefix misc_feature 64..819 /label=BBa_C0062 misc_feature 237..237 /label=T misc_feature 555..555 /label=A misc_feature 820..844 /label=Help:Barcodes misc_feature 853..932 /label=BBa_B0010 stem_loop 864..907 /label=stem_loop misc_feature 941..981 /label=BBa_B0012 stem_loop 948..967 /label=T7 TE polyA_site 968..981 /label=polya misc_feature 974..974 /label=stop misc_feature 990..1009 /label=LuxR/HSL misc_feature 990..1044 /label=BBa_R0062 promoter 1009..1014 /label=-35 promoter 1031..1036 /label=-10 misc_feature 1042..1042 /label=start misc_feature 1..1044 /label=BBa_J09855 misc_feature 1053..1067 /label=BBa_B0030 RBS 1053..1067 /label=RBS-1\Strong RBS 1060..1064 /label=RBS misc_feature 1074..1691 /label=BBa_C0061 CDS 1074..1652 /label=luxI misc_feature 1653..1684 /label=LVA misc_feature 1692..1716 /label=Help:Barcodes misc_feature 1053..1716 /label=BBa_K081008 promoter 1..1716 /label=BBa_K1513000 RBS 1725..1739 /label=RBS-1\Strong RBS 1732..1736 /label=RBS misc_feature 1725..1739 /label=BBa_B0030 RBS 1725..1739 /label=BBa_B0030 promoter 2196..2252 /label=promoter misc_feature 2196..2252 /label=BBa_K346002 RBS 2261..2273 /label=RBS-3\Weak misc_feature 2261..2273 /label=BBa_B0032 RBS 2267..2270 /label=RBS misc_feature 2280..2999 /label=BBa_E0040 misc_feature 3008..3087 /label=BBa_B0010 stem_loop 3019..3062 /label=stem_loop misc_feature 2648..2688 /label=BBa_B0012 stem_loop 3103..3122 /label=T7 TE polyA_site 3123..3136 /label=polya misc_feature 3129..3129 /label=stop misc_feature 2261..3136 /label=BBa_E0240 CDS 1748..2195 /label=merR CDS 1748..3144 /label=BBa_K1513002 ORIGIN 1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac 61 tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt 121 agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa 181 tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta 241 gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat 301 cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac 361 aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc 421 actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca 481 cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca 541 ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca 601 aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc 661 tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc 721 aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta 781 acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga 841 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 961 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa 1021 aatggtttgt tatagtcgaa taaatactag agattaaaga ggagaaatac tagatgacta 1081 taatgataaa aaaatcggat tttttggcaa ttccatcgga ggagtataaa ggtattctaa 1141 gtcttcgtta tcaagtgttt aagcaaagac ttgagtggga cttagttgta gaaaataacc 1201 ttgaatcaga tgagtatgat aactcaaatg cagaatatat ttatgcttgt gatgatactg 1261 aaaatgtaag tggatgctgg cgtttattac ctacaacagg tgattatatg ctgaaaagtg 1321 tttttcctga attgcttggt caacagagtg ctcccaaaga tcctaatata gtcgaattaa 1381 gtcgttttgc tgtaggtaaa aatagctcaa agataaataa ctctgctagt gaaattacaa 1441 tgaaactatt tgaagctata tataaacacg ctgttagtca aggtattaca gaatatgtaa 1501 cagtaacatc aacagcaata gagcgatttt taaagcgtat taaagttcct tgtcatcgta 1561 ttggagacaa agaaattcat gtattaggtg atactaaatc ggttgtattg tctatgccta 1621 ttaatgaaca gtttaaaaaa gcagtcttaa atgctgcaaa cgacgaaaac tacgctttag 1681 tagcttaata actctgatag tgctagtgta gatctctact agagattaaa gaggagaaat 1741 actagaggga gtcaaacgat atggaaaaca atttggagaa tctgactatc ggcgtattcg 1801 ccaaggcggc cggggtcaat gtggaaacca tccggttcta ccaacgcaag ggcttgctgc 1861 ctgagccgga gaagccctat ggcggcatcc gccgttacgg tgacgccgat gtggcgcgcg 1921 tgcggttcgt gaaatcagcc cagcggctgg gcttcagcct ggatgagatc gccgagctgc 1981 tgcggctgga ggatggcacc cattgcgagg aagccagcag tctggccgag cacaagctca 2041 aggacgtgcg cgagaaaatg gctgacctgg cgcgcatgga ggccgtgctg tctgatttgg 2101 tgtgcgcctg ccatgcgcgg aaggggaacg tttcctgccc gctgattgcg tcactgcaag 2161 ggaagaaaga accgcgcagt gcggacgcgg tgtagtacta gagttccata tcgcttgact 2221 ccgtacatga gtacggaagt aaggttacgc tatccaatcc tactagagtc acacaggaaa 2281 gtactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt 2341 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac 2401 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc 2461 aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat 2521 gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat 2581 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac 2641 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg 2701 acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa 2761 gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact 2821 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa 2881 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat 2941 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa 3001 ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 3061 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 3121 cgggtgggcc tttctgcgtt tata //