##gff-version 3
##sequence-region BBa_K1513001 1 3144
BBa_K1513001	.	ribosome_entry_site	1725	1739	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1513001	.	ribosome_entry_site	1732	1736	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1513001	.	ribosome_entry_site	1725	1739	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1513001	.	engineered_region	1725	1739	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1513001	.	ribosome_entry_site	1053	1067	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1513001	.	ribosome_entry_site	1060	1064	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1513001	.	ribosome_entry_site	1053	1067	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1513001	.	engineered_region	1053	1067	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1513001	.	CDS	1748	3144	.	+	0	ID=BBa_K1513002;Name=BBa_K1513002
BBa_K1513001	.	engineered_region	3008	3087	.	+	0	ID=annotation2395376;Name=BBa_B0010;Parent=BBa_K1513002
BBa_K1513001	.	polyA_site	3123	3136	.	+	0	ID=annotation2395380;Name=polya;Parent=BBa_K1513002
BBa_K1513001	.	engineered_region	2196	2252	.	+	0	ID=annotation2395371;Name=BBa_K346002;Parent=BBa_K1513002
BBa_K1513001	.	CDS	1748	2195	.	+	0	ID=annotation2395421;Name=merR;Parent=BBa_K1513002
BBa_K1513001	.	ribosome_entry_site	2261	2273	.	+	0	ID=annotation2395372;Name=RBS-3\Weak;Parent=BBa_K1513002
BBa_K1513001	.	engineered_region	2648	2688	.	+	0	ID=annotation2395378;Name=BBa_B0012;Parent=BBa_K1513002
BBa_K1513001	.	engineered_region	2261	3136	.	+	0	ID=annotation2395382;Name=BBa_E0240;Parent=BBa_K1513002
BBa_K1513001	.	stem_loop	3103	3122	.	+	0	ID=annotation2395379;Name=T7 TE;Parent=BBa_K1513002
BBa_K1513001	.	engineered_region	2261	2273	.	+	0	ID=annotation2395373;Name=BBa_B0032;Parent=BBa_K1513002
BBa_K1513001	.	promoter	2196	2252	.	+	0	ID=annotation2395370;Name=promoter;Parent=BBa_K1513002
BBa_K1513001	.	stop_codon	3129	3129	.	+	0	ID=annotation2395381;Name=stop;Parent=BBa_K1513002
BBa_K1513001	.	ribosome_entry_site	2267	2270	.	+	0	ID=annotation2395374;Name=RBS;Parent=BBa_K1513002
BBa_K1513001	.	stem_loop	3019	3062	.	+	0	ID=annotation2395377;Name=stem_loop;Parent=BBa_K1513002
BBa_K1513001	.	engineered_region	2280	2999	.	+	0	ID=annotation2395375;Name=BBa_E0040;Parent=BBa_K1513002
BBa_K1513001	.	promoter	1	1716	.	+	0	ID=BBa_K1513000;Name=BBa_K1513000
BBa_K1513001	.	start_codon	1042	1042	.	+	0	ID=annotation2397502;Name=start;Parent=BBa_K1513000
BBa_K1513001	.	ribosome_entry_site	1060	1064	.	+	0	ID=annotation2397506;Name=RBS;Parent=BBa_K1513000
BBa_K1513001	.	engineered_region	1074	1691	.	+	0	ID=annotation2397507;Name=BBa_C0061;Parent=BBa_K1513000
BBa_K1513001	.	engineered_region	1053	1716	.	+	0	ID=annotation2397511;Name=BBa_K081008;Parent=BBa_K1513000
BBa_K1513001	.	point_mutation	237	237	.	+	0	ID=annotation2397489;Name=T;Parent=BBa_K1513000
BBa_K1513001	.	stem_loop	948	967	.	+	0	ID=annotation2397495;Name=T7 TE;Parent=BBa_K1513000
BBa_K1513001	.	conserved_region	50	53	.	+	0	ID=annotation2397485;Name=conserved;Parent=BBa_K1513000
BBa_K1513001	.	engineered_region	1	37	.	+	0	ID=annotation2397480;Name=BBa_I14032;Parent=BBa_K1513000
BBa_K1513001	.	engineered_region	46	57	.	+	0	ID=annotation2397484;Name=BBa_B0034;Parent=BBa_K1513000
BBa_K1513001	.	engineered_region	1	1044	.	+	0	ID=annotation2397503;Name=BBa_J09855;Parent=BBa_K1513000
BBa_K1513001	.	point_mutation	555	555	.	+	0	ID=annotation2397490;Name=A;Parent=BBa_K1513000
BBa_K1513001	.	engineered_region	990	1044	.	+	0	ID=annotation2397499;Name=BBa_R0062;Parent=BBa_K1513000
BBa_K1513001	.	stop_codon	974	974	.	+	0	ID=annotation2397497;Name=stop;Parent=BBa_K1513000
BBa_K1513001	.	tag	1653	1684	.	+	0	ID=annotation2397509;Name=LVA;Parent=BBa_K1513000
BBa_K1513001	.	engineered_region	64	819	.	+	0	ID=annotation2397488;Name=BBa_C0062;Parent=BBa_K1513000
BBa_K1513001	.	promoter	26	31	.	+	0	ID=annotation2397482;Name=-10;Parent=BBa_K1513000
BBa_K1513001	.	engineered_region	941	981	.	+	0	ID=annotation2397494;Name=BBa_B0012;Parent=BBa_K1513000
BBa_K1513001	.	promoter	3	8	.	+	0	ID=annotation2397481;Name=-35;Parent=BBa_K1513000
BBa_K1513001	.	CDS	64	813	.	+	0	ID=annotation2397486;Name=luxR;Parent=BBa_K1513000
BBa_K1513001	.	engineered_tag	820	844	.	+	0	ID=annotation2397491;Name=Help:Barcodes;Parent=BBa_K1513000
BBa_K1513001	.	non_covalent_binding_site	990	1009	.	+	0	ID=annotation2397498;Name=LuxR/HSL;Parent=BBa_K1513000
BBa_K1513001	.	engineered_region	1053	1067	.	+	0	ID=annotation2397504;Name=BBa_B0030;Parent=BBa_K1513000
BBa_K1513001	.	polyA_site	968	981	.	+	0	ID=annotation2397496;Name=polya;Parent=BBa_K1513000
BBa_K1513001	.	stem_loop	864	907	.	+	0	ID=annotation2397493;Name=stem_loop;Parent=BBa_K1513000
BBa_K1513001	.	ribosome_entry_site	1053	1067	.	+	0	ID=annotation2397505;Name=RBS-1\Strong;Parent=BBa_K1513000
BBa_K1513001	.	CDS	1074	1652	.	+	0	ID=annotation2397508;Name=luxI;Parent=BBa_K1513000
BBa_K1513001	.	promoter	1031	1036	.	+	0	ID=annotation2397501;Name=-10;Parent=BBa_K1513000
BBa_K1513001	.	operator	1	37	.	+	0	ID=annotation2397479;Name=P(Lac) IQ;Parent=BBa_K1513000
BBa_K1513001	.	engineered_region	853	932	.	+	0	ID=annotation2397492;Name=BBa_B0010;Parent=BBa_K1513000
BBa_K1513001	.	engineered_region	46	981	.	+	0	ID=annotation2397483;Name=BBa_I0462;Parent=BBa_K1513000
BBa_K1513001	.	engineered_tag	1692	1716	.	+	0	ID=annotation2397510;Name=Help:Barcodes;Parent=BBa_K1513000
BBa_K1513001	.	engineered_region	64	65	.	+	0	ID=annotation2397487;Name=prefix;Parent=BBa_K1513000
BBa_K1513001	.	promoter	1009	1014	.	+	0	ID=annotation2397500;Name=-35;Parent=BBa_K1513000
>BBa_K1513001
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaacataaatg
ccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatat
gactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatc
ctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattatt
ctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaa
agaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagt
tttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttcctt
ctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatg
tttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttc
catttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaa
ttgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtt
tgttatagtcgaataaatactagagattaaagaggagaaatactagatgactataatgataaaaaaatcggattttttg
gcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttag
ttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaa
tgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaa
cagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataact
ctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaac
agtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcat
gtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaa
acgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagattaaagaggagaa
atactagagggagtcaaacgatatggaaaacaatttggagaatctgactatcggcgtattcgccaaggcggccggggtc
aatgtggaaaccatccggttctaccaacgcaagggcttgctgcctgagccggagaagccctatggcggcatccgccgtt
acggtgacgccgatgtggcgcgcgtgcggttcgtgaaatcagcccagcggctgggcttcagcctggatgagatcgccga
gctgctgcggctggaggatggcacccattgcgaggaagccagcagtctggccgagcacaagctcaaggacgtgcgcgag
aaaatggctgacctggcgcgcatggaggccgtgctgtctgatttggtgtgcgcctgccatgcgcggaaggggaacgttt
cctgcccgctgattgcgtcactgcaagggaagaaagaaccgcgcagtgcggacgcggtgtagtactagagttccatatc
gcttgactccgtacatgagtacggaagtaaggttacgctatccaatcctactagagtcacacaggaaagtactagatgc
gtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttc
tgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacct
gttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagc
atgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaa
gacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagat
ggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatg
gaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatac
tccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaac
gaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaat
aataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgt
cggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata