BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1702 1 RBS range1702 1 8 12 annotation1701 1 RBS-1\Strong range1701 1 1 15 BBa_K1513002 1 BBa_K1513002 Hg(II) sensor 2014-09-30T11:00:00Z 2015-05-08T01:10:48Z 2q 1q false false _1893_ 0 21294 9 It's complicated false 3q false Hongwei Duan annotation2395375 1 BBa_E0040 range2395375 1 533 1252 annotation2395372 1 RBS-3\Weak range2395372 1 514 526 annotation2395370 1 promoter range2395370 1 449 505 annotation2395421 1 merR range2395421 1 1 448 annotation2395381 1 stop range2395381 1 1382 1382 annotation2395378 1 BBa_B0012 range2395378 1 901 941 annotation2395377 1 stem_loop range2395377 1 1272 1315 annotation2395380 1 polya range2395380 1 1376 1389 annotation2395371 1 BBa_K346002 range2395371 1 449 505 annotation2395374 1 RBS range2395374 1 520 523 annotation2395373 1 BBa_B0032 range2395373 1 514 526 annotation2395382 1 BBa_E0240 range2395382 1 514 1389 annotation2395379 1 T7 TE range2395379 1 1356 1375 annotation2395376 1 BBa_B0010 range2395376 1 1261 1340 BBa_K1513001 1 BBa_K1513001 Fast-type merR biosensor 2014-09-25T11:00:00Z 2015-05-08T01:10:48Z qq qq false false _1893_ 0 21294 9 It's complicated false qq false Hongwei Duan component2405585 1 BBa_K1513002 component2405569 1 BBa_B0030 component2405567 1 BBa_K1513000 component2405560 1 BBa_B0030 annotation2405569 1 BBa_B0030 range2405569 1 1725 1739 annotation2405567 1 BBa_K1513000 range2405567 1 1 1716 annotation2405560 1 BBa_B0030 range2405560 1 1053 1067 annotation2405585 1 BBa_K1513002 range2405585 1 1748 3144 BBa_K1513000 1 BBa_K1513000 A quorum sensing system which can produce auto-inducer (AHL) itself. 2014-09-17T11:00:00Z 2015-05-08T01:10:48Z qq qq false false _1893_ 0 21294 9 It's complicated false qq false Hongwei Duan annotation2397504 1 BBa_B0030 range2397504 1 1053 1067 annotation2397507 1 BBa_C0061 range2397507 1 1074 1691 annotation2397499 1 BBa_R0062 range2397499 1 990 1044 annotation2397479 1 P(Lac) IQ range2397479 1 1 37 annotation2397505 1 RBS-1\Strong range2397505 1 1053 1067 annotation2397480 1 BBa_I14032 range2397480 1 1 37 annotation2397489 1 T range2397489 1 237 237 annotation2397488 1 BBa_C0062 range2397488 1 64 819 annotation2397503 1 BBa_J09855 range2397503 1 1 1044 annotation2397481 1 -35 range2397481 1 3 8 annotation2397506 1 RBS range2397506 1 1060 1064 annotation2397494 1 BBa_B0012 range2397494 1 941 981 annotation2397491 1 Help:Barcodes range2397491 1 820 844 annotation2397511 1 BBa_K081008 range2397511 1 1053 1716 annotation2397484 1 BBa_B0034 range2397484 1 46 57 annotation2397510 1 Help:Barcodes range2397510 1 1692 1716 annotation2397502 1 start range2397502 1 1042 1042 annotation2397497 1 stop range2397497 1 974 974 annotation2397486 1 luxR range2397486 1 64 813 annotation2397501 1 -10 range2397501 1 1031 1036 annotation2397485 1 conserved range2397485 1 50 53 annotation2397495 1 T7 TE range2397495 1 948 967 annotation2397498 1 LuxR/HSL range2397498 1 990 1009 annotation2397496 1 polya range2397496 1 968 981 annotation2397508 1 luxI range2397508 1 1074 1652 annotation2397493 1 stem_loop range2397493 1 864 907 annotation2397482 1 -10 range2397482 1 26 31 annotation2397509 1 LVA range2397509 1 1653 1684 annotation2397483 1 BBa_I0462 range2397483 1 46 981 annotation2397500 1 -35 range2397500 1 1009 1014 annotation2397492 1 BBa_B0010 range2397492 1 853 932 annotation2397487 1 prefix range2397487 1 64 65 annotation2397490 1 A range2397490 1 555 555 BBa_K1513000_sequence 1 tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagattaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc BBa_K1513001_sequence 1 tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagattaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagattaaagaggagaaatactagagggagtcaaacgatatggaaaacaatttggagaatctgactatcggcgtattcgccaaggcggccggggtcaatgtggaaaccatccggttctaccaacgcaagggcttgctgcctgagccggagaagccctatggcggcatccgccgttacggtgacgccgatgtggcgcgcgtgcggttcgtgaaatcagcccagcggctgggcttcagcctggatgagatcgccgagctgctgcggctggaggatggcacccattgcgaggaagccagcagtctggccgagcacaagctcaaggacgtgcgcgagaaaatggctgacctggcgcgcatggaggccgtgctgtctgatttggtgtgcgcctgccatgcgcggaaggggaacgtttcctgcccgctgattgcgtcactgcaagggaagaaagaaccgcgcagtgcggacgcggtgtagtactagagttccatatcgcttgactccgtacatgagtacggaagtaaggttacgctatccaatcctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0030_sequence 1 attaaagaggagaaa BBa_K1513002_sequence 1 ggagtcaaacgatatggaaaacaatttggagaatctgactatcggcgtattcgccaaggcggccggggtcaatgtggaaaccatccggttctaccaacgcaagggcttgctgcctgagccggagaagccctatggcggcatccgccgttacggtgacgccgatgtggcgcgcgtgcggttcgtgaaatcagcccagcggctgggcttcagcctggatgagatcgccgagctgctgcggctggaggatggcacccattgcgaggaagccagcagtctggccgagcacaagctcaaggacgtgcgcgagaaaatggctgacctggcgcgcatggaggccgtgctgtctgatttggtgtgcgcctgccatgcgcggaaggggaacgtttcctgcccgctgattgcgtcactgcaagggaagaaagaaccgcgcagtgcggacgcggtgtagtactagagttccatatcgcttgactccgtacatgagtacggaagtaaggttacgctatccaatcctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z