BBa_K1513001
type: ComponentDefinition
persistent identity: BBa K1513001
display id: BBa_K1513001
version: 1
was generated by: iGEM to SBOL conversion
description: Fast-type merR biosensor
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:48Z
mutable provenance: qq
owned by: james
owned by: myers
mutable description: qq
discontinued: false
dominant: false
experience: experience/None
group u list: _1893_
m user id: 0
owner id: 21294
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: qq
star: false
creator: Hongwei Duan
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1513002
component: BBa_B0030
component: BBa_K1513000
sequence annotation: BBa_K1513000
sequence annotation: BBa_B0030
sequence annotation: BBa_K1513002
sequence: https://synbiohub.org/public/igem/BBa_K1513001_sequence/1

BBa_K1513002
type: Component
persistent identity: component2405585
display id: component2405585
version: 1
top level: BBa_K1513001
definition: BBa_K1513002
access: public

BBa_B0030
type: Component
persistent identity: component2405569
display id: component2405569
version: 1
top level: BBa_K1513001
definition: BBa_B0030
access: public

BBa_K1513000
type: Component
persistent identity: component2405567
display id: component2405567
version: 1
top level: BBa_K1513001
definition: BBa_K1513000
access: public

BBa_B0030
type: Component
persistent identity: component2405560
display id: component2405560
version: 1
top level: BBa_K1513001
definition: BBa_B0030
access: public

BBa_K1513000
type: SequenceAnnotation
persistent identity: annotation2405567
display id: annotation2405567
version: 1
top level: BBa_K1513001
location: https://synbiohub.org/public/igem/BBa_K1513001/annotation2405567/range2405567/1
component: BBa_K1513000

https://synbiohub.org/public/igem/BBa_K1513001/annotation2405567/range2405567/1
type: Range
persistent identity: range2405567
display id: range2405567
version: 1
top level: BBa_K1513001
direction: direction/reverse
start: 1
end: 1716
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2405560
display id: annotation2405560
version: 1
top level: BBa_K1513001
location: https://synbiohub.org/public/igem/BBa_K1513001/annotation2405560/range2405560/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K1513001/annotation2405560/range2405560/1
type: Range
persistent identity: range2405560
display id: range2405560
version: 1
top level: BBa_K1513001
direction: direction/forward
start: 1053
end: 1067
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2405569
display id: annotation2405569
version: 1
top level: BBa_K1513001
location: https://synbiohub.org/public/igem/BBa_K1513001/annotation2405569/range2405569/1
component: BBa_B0030

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type: Range
persistent identity: range2405569
display id: range2405569
version: 1
top level: BBa_K1513001
direction: direction/forward
start: 1725
end: 1739
orientation: inline

BBa_K1513002
type: SequenceAnnotation
persistent identity: annotation2405585
display id: annotation2405585
version: 1
top level: BBa_K1513001
location: https://synbiohub.org/public/igem/BBa_K1513001/annotation2405585/range2405585/1
component: BBa_K1513002

https://synbiohub.org/public/igem/BBa_K1513001/annotation2405585/range2405585/1
type: Range
persistent identity: range2405585
display id: range2405585
version: 1
top level: BBa_K1513001
direction: direction/reverse
start: 1748
end: 3144
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_K1513000
type: ComponentDefinition
persistent identity: BBa K1513000
display id: BBa_K1513000
version: 1
was generated by: iGEM to SBOL conversion
description: A quorum sensing system which can produce auto-inducer (AHL) itself.
created: 2014-09-17T11:00:00Z
modified: 2015-05-08T01:10:48Z
mutable provenance: qq
owned by: james
owned by: myers
mutable description: qq
discontinued: false
dominant: false
experience: experience/None
group u list: _1893_
m user id: 0
owner id: 21294
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: qq
star: false
creator: Hongwei Duan
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_I14032
sequence annotation: P(Lac) IQ
sequence annotation: BBa_J09855
sequence annotation: -35
sequence annotation: -10
sequence annotation: BBa_B0034
sequence annotation: BBa_I0462
sequence annotation: conserved
sequence annotation: BBa_C0062
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence annotation: LuxR/HSL
sequence annotation: BBa_R0062
sequence annotation: start
sequence annotation: BBa_K081008
sequence annotation: BBa_B0030
sequence annotation: RBS-1\Strong
sequence annotation: RBS
sequence annotation: luxI
sequence annotation: BBa_C0061
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_K1513000_sequence/1

BBa_I14032
type: SequenceAnnotation
persistent identity: annotation2397480
display id: annotation2397480
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397480/range2397480/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1513000/annotation2397480/range2397480/1
type: Range
persistent identity: range2397480
display id: range2397480
version: 1
top level: BBa_K1513000
direction: direction/reverse
start: 1
end: 37
orientation: inline

P(Lac) IQ
type: SequenceAnnotation
persistent identity: annotation2397479
display id: annotation2397479
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397479/range2397479/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_K1513000/annotation2397479/range2397479/1
type: Range
persistent identity: range2397479
display id: range2397479
version: 1
top level: BBa_K1513000
direction: direction/forward
start: 1
end: 37
orientation: inline

BBa_J09855
type: SequenceAnnotation
persistent identity: annotation2397503
display id: annotation2397503
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397503/range2397503/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1513000/annotation2397503/range2397503/1
type: Range
persistent identity: range2397503
display id: range2397503
version: 1
top level: BBa_K1513000
direction: direction/reverse
start: 1
end: 1044
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2397481
display id: annotation2397481
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397481/range2397481/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K1513000/annotation2397481/range2397481/1
type: Range
persistent identity: range2397481
display id: range2397481
version: 1
top level: BBa_K1513000
direction: direction/forward
start: 3
end: 8
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2397482
display id: annotation2397482
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397482/range2397482/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K1513000/annotation2397482/range2397482/1
type: Range
persistent identity: range2397482
display id: range2397482
version: 1
top level: BBa_K1513000
direction: direction/forward
start: 26
end: 31
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2397484
display id: annotation2397484
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397484/range2397484/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1513000/annotation2397484/range2397484/1
type: Range
persistent identity: range2397484
display id: range2397484
version: 1
top level: BBa_K1513000
direction: direction/forward
start: 46
end: 57
orientation: inline

BBa_I0462
type: SequenceAnnotation
persistent identity: annotation2397483
display id: annotation2397483
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397483/range2397483/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1513000/annotation2397483/range2397483/1
type: Range
persistent identity: range2397483
display id: range2397483
version: 1
top level: BBa_K1513000
direction: direction/reverse
start: 46
end: 981
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2397485
display id: annotation2397485
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397485/range2397485/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_K1513000/annotation2397485/range2397485/1
type: Range
persistent identity: range2397485
display id: range2397485
version: 1
top level: BBa_K1513000
direction: direction/forward
start: 50
end: 53
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2397488
display id: annotation2397488
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397488/range2397488/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1513000/annotation2397488/range2397488/1
type: Range
persistent identity: range2397488
display id: range2397488
version: 1
top level: BBa_K1513000
direction: direction/forward
start: 64
end: 819
orientation: inline

luxR
type: SequenceAnnotation
persistent identity: annotation2397486
display id: annotation2397486
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397486/range2397486/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1513000/annotation2397486/range2397486/1
type: Range
persistent identity: range2397486
display id: range2397486
version: 1
top level: BBa_K1513000
direction: direction/forward
start: 64
end: 813
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2397487
display id: annotation2397487
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397487/range2397487/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1513000/annotation2397487/range2397487/1
type: Range
persistent identity: range2397487
display id: range2397487
version: 1
top level: BBa_K1513000
direction: direction/forward
start: 64
end: 65
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation2397489
display id: annotation2397489
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397489/range2397489/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K1513000/annotation2397489/range2397489/1
type: Range
persistent identity: range2397489
display id: range2397489
version: 1
top level: BBa_K1513000
direction: direction/forward
start: 237
end: 237
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation2397490
display id: annotation2397490
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397490/range2397490/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K1513000/annotation2397490/range2397490/1
type: Range
persistent identity: range2397490
display id: range2397490
version: 1
top level: BBa_K1513000
direction: direction/forward
start: 555
end: 555
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2397491
display id: annotation2397491
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397491/range2397491/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_K1513000/annotation2397491/range2397491/1
type: Range
persistent identity: range2397491
display id: range2397491
version: 1
top level: BBa_K1513000
direction: direction/forward
start: 820
end: 844
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2397492
display id: annotation2397492
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397492/range2397492/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1513000/annotation2397492/range2397492/1
type: Range
persistent identity: range2397492
display id: range2397492
version: 1
top level: BBa_K1513000
direction: direction/forward
start: 853
end: 932
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2397493
display id: annotation2397493
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397493/range2397493/1
role: SO:0000313
role: feature/stem loop

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type: Range
persistent identity: range2397493
display id: range2397493
version: 1
top level: BBa_K1513000
direction: direction/forward
start: 864
end: 907
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2397494
display id: annotation2397494
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397494/range2397494/1
role: SO:0000804
role: feature/BioBrick

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type: Range
persistent identity: range2397494
display id: range2397494
version: 1
top level: BBa_K1513000
direction: direction/forward
start: 941
end: 981
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2397495
display id: annotation2397495
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397495/range2397495/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_K1513000/annotation2397495/range2397495/1
type: Range
persistent identity: range2397495
display id: range2397495
version: 1
top level: BBa_K1513000
direction: direction/forward
start: 948
end: 967
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2397496
display id: annotation2397496
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397496/range2397496/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_K1513000/annotation2397496/range2397496/1
type: Range
persistent identity: range2397496
display id: range2397496
version: 1
top level: BBa_K1513000
direction: direction/forward
start: 968
end: 981
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2397497
display id: annotation2397497
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397497/range2397497/1
role: SO:0000319
role: feature/stop

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type: Range
persistent identity: range2397497
display id: range2397497
version: 1
top level: BBa_K1513000
direction: direction/forward
start: 974
end: 974
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2397498
display id: annotation2397498
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397498/range2397498/1
role: feature/binding
role: SO:0001091

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type: Range
persistent identity: range2397498
display id: range2397498
version: 1
top level: BBa_K1513000
direction: direction/forward
start: 990
end: 1009
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2397499
display id: annotation2397499
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397499/range2397499/1
role: feature/BioBrick
role: SO:0000804

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type: Range
persistent identity: range2397499
display id: range2397499
version: 1
top level: BBa_K1513000
direction: direction/forward
start: 990
end: 1044
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2397500
display id: annotation2397500
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397500/range2397500/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K1513000/annotation2397500/range2397500/1
type: Range
persistent identity: range2397500
display id: range2397500
version: 1
top level: BBa_K1513000
direction: direction/forward
start: 1009
end: 1014
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2397501
display id: annotation2397501
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397501/range2397501/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K1513000/annotation2397501/range2397501/1
type: Range
persistent identity: range2397501
display id: range2397501
version: 1
top level: BBa_K1513000
direction: direction/forward
start: 1031
end: 1036
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2397502
display id: annotation2397502
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397502/range2397502/1
role: SO:0000318
role: feature/start

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type: Range
persistent identity: range2397502
display id: range2397502
version: 1
top level: BBa_K1513000
direction: direction/forward
start: 1042
end: 1042
orientation: inline

BBa_K081008
type: SequenceAnnotation
persistent identity: annotation2397511
display id: annotation2397511
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397511/range2397511/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1513000/annotation2397511/range2397511/1
type: Range
persistent identity: range2397511
display id: range2397511
version: 1
top level: BBa_K1513000
direction: direction/reverse
start: 1053
end: 1716
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2397504
display id: annotation2397504
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397504/range2397504/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1513000/annotation2397504/range2397504/1
type: Range
persistent identity: range2397504
display id: range2397504
version: 1
top level: BBa_K1513000
direction: direction/forward
start: 1053
end: 1067
orientation: inline

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation2397505
display id: annotation2397505
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397505/range2397505/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1513000/annotation2397505/range2397505/1
type: Range
persistent identity: range2397505
display id: range2397505
version: 1
top level: BBa_K1513000
direction: direction/forward
start: 1053
end: 1067
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2397506
display id: annotation2397506
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397506/range2397506/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1513000/annotation2397506/range2397506/1
type: Range
persistent identity: range2397506
display id: range2397506
version: 1
top level: BBa_K1513000
direction: direction/forward
start: 1060
end: 1064
orientation: inline

luxI
type: SequenceAnnotation
persistent identity: annotation2397508
display id: annotation2397508
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397508/range2397508/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1513000/annotation2397508/range2397508/1
type: Range
persistent identity: range2397508
display id: range2397508
version: 1
top level: BBa_K1513000
direction: direction/forward
start: 1074
end: 1652
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2397507
display id: annotation2397507
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397507/range2397507/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1513000/annotation2397507/range2397507/1
type: Range
persistent identity: range2397507
display id: range2397507
version: 1
top level: BBa_K1513000
direction: direction/forward
start: 1074
end: 1691
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation2397509
display id: annotation2397509
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397509/range2397509/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K1513000/annotation2397509/range2397509/1
type: Range
persistent identity: range2397509
display id: range2397509
version: 1
top level: BBa_K1513000
direction: direction/forward
start: 1653
end: 1684
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2397510
display id: annotation2397510
version: 1
top level: BBa_K1513000
location: https://synbiohub.org/public/igem/BBa_K1513000/annotation2397510/range2397510/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_K1513000/annotation2397510/range2397510/1
type: Range
persistent identity: range2397510
display id: range2397510
version: 1
top level: BBa_K1513000
direction: direction/forward
start: 1692
end: 1716
orientation: inline

BBa_K1513002
type: ComponentDefinition
persistent identity: BBa K1513002
display id: BBa_K1513002
version: 1
was generated by: iGEM to SBOL conversion
description: Hg(II) sensor
created: 2014-09-30T11:00:00Z
modified: 2015-05-08T01:10:48Z
mutable provenance: 2q
owned by: james
owned by: myers
mutable description: 1q
discontinued: false
dominant: false
experience: experience/None
group u list: _1893_
m user id: 0
owner id: 21294
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: 3q
star: false
creator: Hongwei Duan
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: merR
sequence annotation: BBa_K346002
sequence annotation: promoter
sequence annotation: BBa_E0240
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1513002_sequence/1

merR
type: SequenceAnnotation
persistent identity: annotation2395421
display id: annotation2395421
version: 1
top level: BBa_K1513002
location: https://synbiohub.org/public/igem/BBa_K1513002/annotation2395421/range2395421/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1513002/annotation2395421/range2395421/1
type: Range
persistent identity: range2395421
display id: range2395421
version: 1
top level: BBa_K1513002
direction: direction/forward
start: 1
end: 448
orientation: inline

BBa_K346002
type: SequenceAnnotation
persistent identity: annotation2395371
display id: annotation2395371
version: 1
top level: BBa_K1513002
location: https://synbiohub.org/public/igem/BBa_K1513002/annotation2395371/range2395371/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1513002/annotation2395371/range2395371/1
type: Range
persistent identity: range2395371
display id: range2395371
version: 1
top level: BBa_K1513002
direction: direction/reverse
start: 449
end: 505
orientation: inline

promoter
type: SequenceAnnotation
persistent identity: annotation2395370
display id: annotation2395370
version: 1
top level: BBa_K1513002
location: https://synbiohub.org/public/igem/BBa_K1513002/annotation2395370/range2395370/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K1513002/annotation2395370/range2395370/1
type: Range
persistent identity: range2395370
display id: range2395370
version: 1
top level: BBa_K1513002
direction: direction/forward
start: 449
end: 505
orientation: inline

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2395382
display id: annotation2395382
version: 1
top level: BBa_K1513002
location: https://synbiohub.org/public/igem/BBa_K1513002/annotation2395382/range2395382/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1513002/annotation2395382/range2395382/1
type: Range
persistent identity: range2395382
display id: range2395382
version: 1
top level: BBa_K1513002
direction: direction/reverse
start: 514
end: 1389
orientation: inline

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation2395372
display id: annotation2395372
version: 1
top level: BBa_K1513002
location: https://synbiohub.org/public/igem/BBa_K1513002/annotation2395372/range2395372/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1513002/annotation2395372/range2395372/1
type: Range
persistent identity: range2395372
display id: range2395372
version: 1
top level: BBa_K1513002
direction: direction/forward
start: 514
end: 526
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2395373
display id: annotation2395373
version: 1
top level: BBa_K1513002
location: https://synbiohub.org/public/igem/BBa_K1513002/annotation2395373/range2395373/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1513002/annotation2395373/range2395373/1
type: Range
persistent identity: range2395373
display id: range2395373
version: 1
top level: BBa_K1513002
direction: direction/forward
start: 514
end: 526
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2395374
display id: annotation2395374
version: 1
top level: BBa_K1513002
location: https://synbiohub.org/public/igem/BBa_K1513002/annotation2395374/range2395374/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1513002/annotation2395374/range2395374/1
type: Range
persistent identity: range2395374
display id: range2395374
version: 1
top level: BBa_K1513002
direction: direction/forward
start: 520
end: 523
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2395375
display id: annotation2395375
version: 1
top level: BBa_K1513002
location: https://synbiohub.org/public/igem/BBa_K1513002/annotation2395375/range2395375/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1513002/annotation2395375/range2395375/1
type: Range
persistent identity: range2395375
display id: range2395375
version: 1
top level: BBa_K1513002
direction: direction/forward
start: 533
end: 1252
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2395378
display id: annotation2395378
version: 1
top level: BBa_K1513002
location: https://synbiohub.org/public/igem/BBa_K1513002/annotation2395378/range2395378/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1513002/annotation2395378/range2395378/1
type: Range
persistent identity: range2395378
display id: range2395378
version: 1
top level: BBa_K1513002
direction: direction/forward
start: 901
end: 941
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2395376
display id: annotation2395376
version: 1
top level: BBa_K1513002
location: https://synbiohub.org/public/igem/BBa_K1513002/annotation2395376/range2395376/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1513002/annotation2395376/range2395376/1
type: Range
persistent identity: range2395376
display id: range2395376
version: 1
top level: BBa_K1513002
direction: direction/forward
start: 1261
end: 1340
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2395377
display id: annotation2395377
version: 1
top level: BBa_K1513002
location: https://synbiohub.org/public/igem/BBa_K1513002/annotation2395377/range2395377/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_K1513002/annotation2395377/range2395377/1
type: Range
persistent identity: range2395377
display id: range2395377
version: 1
top level: BBa_K1513002
direction: direction/forward
start: 1272
end: 1315
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2395379
display id: annotation2395379
version: 1
top level: BBa_K1513002
location: https://synbiohub.org/public/igem/BBa_K1513002/annotation2395379/range2395379/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K1513002/annotation2395379/range2395379/1
type: Range
persistent identity: range2395379
display id: range2395379
version: 1
top level: BBa_K1513002
direction: direction/forward
start: 1356
end: 1375
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2395380
display id: annotation2395380
version: 1
top level: BBa_K1513002
location: https://synbiohub.org/public/igem/BBa_K1513002/annotation2395380/range2395380/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_K1513002/annotation2395380/range2395380/1
type: Range
persistent identity: range2395380
display id: range2395380
version: 1
top level: BBa_K1513002
direction: direction/forward
start: 1376
end: 1389
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2395381
display id: annotation2395381
version: 1
top level: BBa_K1513002
location: https://synbiohub.org/public/igem/BBa_K1513002/annotation2395381/range2395381/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1513002/annotation2395381/range2395381/1
type: Range
persistent identity: range2395381
display id: range2395381
version: 1
top level: BBa_K1513002
direction: direction/forward
start: 1382
end: 1382
orientation: inline

https://synbiohub.org/public/igem/BBa_K1513001_sequence/1
type: Sequence
persistent identity: BBa K1513001 sequence
display id: BBa_K1513001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagattaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagattaaagaggagaaatactagagggagtcaaacgatatggaaaacaatttggagaatctgactatcggcgtattcgccaaggcggccggggtcaatgtggaaaccatccggttctaccaacgcaagggcttgctgcctgagccggagaagccctatggcggcatccgccgttacggtgacgccgatgtggcgcgcgtgcggttcgtgaaatcagcccagcggctgggcttcagcctggatgagatcgccgagctgctgcggctggaggatggcacc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1513000_sequence/1
type: Sequence
persistent identity: BBa K1513000 sequence
display id: BBa_K1513000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagattaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1513002_sequence/1
type: Sequence
persistent identity: BBa K1513002 sequence
display id: BBa_K1513002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggagtcaaacgatatggaaaacaatttggagaatctgactatcggcgtattcgccaaggcggccggggtcaatgtggaaaccatccggttctaccaacgcaagggcttgctgcctgagccggagaagccctatggcggcatccgccgttacggtgacgccgatgtggcgcgcgtgcggttcgtgaaatcagcccagcggctgggcttcagcctggatgagatcgccgagctgctgcggctggaggatggcacccattgcgaggaagccagcagtctggccgagcacaagctcaaggacgtgcgcgagaaaatggctgacctggcgcgcatggaggccgtgctgtctgatttggtgtgcgcctgccatgcgcggaaggggaacgtttcctgcccgctgattgcgtcactgcaagggaagaaagaaccgcgcagtgcggacgcggtgtagtactagagttccatatcgcttgactccgtacatgagtacggaagtaaggttacgctatccaatcctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z