BBa_K1518000 1 BBa_K1518000 Thread Biopolymer Filament Gamma TBFG_EPTST 2014-09-27T11:00:00Z 2015-05-08T01:10:48Z The part DNA was artificially synthesised based on the mRNA sequence from Koch, et al (1994). Protein is originally expressed by Pacific Hagfish (Eptatretus stoutii). DNA sequence was codon optimised using codon optimisation software and synthesised by Gen9. Thread biopolymer filament subunit gamma (Eptatretus stoutii) This part is intended to be produced as a component of an intermediate filament construct. TBFG codes for the γ protein subunit of the Hagfish thread biopolymer. It is intended to assemble as part of a coiled-coil heterodimer of an α/γ intermediate filament. false false _1900_ 0 21930 9 In stock false Sequence is codon optimised for expression in E. coli. false Ian Valentine McDermott annotation2391828 1 TAA range2391828 1 1810 1812 annotation2387788 1 ATG range2387788 1 1 3 annotation2387789 1 TAA range2387789 1 1807 1809 BBa_K1518000_sequence 1 atggcatcccacagctctgttagctatcgttctgtgcggactggcggcacttcagcgatgatcggttcatccggctacggtggtagcagcagctctcgtgctggcctgggcatgggcgcggctggcctgtcgatgggcgggggctccttccgtgtaggttcggctggtattggtggtatgggtatttcttcaggtattggtggtatgggcatttcctcccgcgctggcggcatgtctgcctacggtggagctgcttctggtggtgctggtgggttcgtttctggtggcgttccgatgctgggttacggcggtggcgctggcggttttatcggcggcgtttcgccgggtatcatggcctccccggcctttaccgccggccgtgcaattacctctgcgggtatgagcggcgttgtcggtactttgggccccgctggcggtatggttccaagtctggtatctcgcgatgaagtaaaaaatatcctgggcactcttaaccagcgcctggcgtcttacgtggataaagtgcgtcagctgacaatcgaaaacgaaactatggaagaagaactgaagaacctgactggtggtgttccgatgtcgccggactctaccgtcaacctggaaaacgtagagactcaggtaaccgagatgcttaccgaagtatccaatctgaccctggagcgtgtccgcctggagattgacgttgaccatctgcgcgcaaccgcggacgaaatcaaaagcaaatacgagtttgaactgggcgttcgtatgcagctggaaaccgacatcgcgaacatgaaacgcgatctggaagcggccaacgacatgcgcgttgacttagatagcaaatttaacttcctgactgaagaactgactttccagcgcaaaactcagatggaagagctgaacactctgaaacaacagttcggtcgcctgggtccggttcaaaccagcgttattgaactcgataacgtaaagtctgtgaacctgaccgatgcgctgaacgtcatgcgtgaggaataccagcaggttgtcaccaaaaatgtacaggaagcagaaacttactgtaaaatgcaaatcgatcagatccagggtatctccacccagactactgaacaaatctctatcctggacaaagagattaacaccttagaaaaggaattacaaccgctgaacgtggagtaccaacgtctcctgaccacctatcagaccctgggcgatcgtctgacagacttacaaaaccgcgaatctattgacctggtacagttccagaacacctacacacgctacgaacaggaaatcgaaggcaaccaggttgacttacagcgtcagctggttacttaccaacagctgctggacgtaaaaactgccctggatgctgaaatcgccacttacaaaaaactgcttgaaggccaggaattgatggttcgtaccgctatggcagacgattttgcacacgcaaccgttgtacgctcgggtactctgggtggtgcaagctccagttcggttggttacggcgcgagctctacgactctgggcgctatctctggtggttattcgacgggcggtggcgcttcttactccgcgggtgcgggtggggctagttactccgctggtgctggtggcgcgtcttacggcgttggtggtggttattctggtggttcttccgccatgatggagggcagctctagcggtgggcactctatgtattctagcagctctatgaaacgttcttcttccaaatctgcaagcgccagtgctggcggttatggcactagcggtcacgacagcaccatcatcttacaacagtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z