LOCUS       BBa_K1519124            1482 bp    DNA     linear       11-May-2024
DEFINITION  MCherry fused to K1519123 for Characterization
ACCESSION   BBa_K1519124
VERSION     BBa_K1519124.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     misc_feature    59..62
                     /label=conserved
     RBS             55..66
                     /label=BBa_B0034
     CDS             73..678
                     /label=osmY
     CDS             73..678
                     /label=BBa_K892008
     misc_feature    687..698
                     /label=Short Linker
     misc_feature    687..698
                     /label=BBa_K243004
     CDS             707..724
                     /label=His-Tag
     misc_feature    707..724
                     /label=BBa_K157011
     misc_feature    733..753
                     /label=BBa_J18918
     CDS             760..1467
                     /label=mCherry
     misc_feature    1111..1111
                     /label=C->T (removing PstI site)
     misc_feature    1468..1476
                     /label=LVA
     misc_feature    760..1482
                     /label=BBa_J06505
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagaaagag
       61 gagaaatact agatgactat gacaagactg aagatttcga aaactctgct ggctgtaatg
      121 ttgacctctg ccgtcgcgac cggctctgcc tacgcggaaa acaacgcgca gactaccaat
      181 gaaagcgcag ggcaaaaagt cgatagctct atgaataaag tcggtaattt catggatgac
      241 agcgccatca ccgcgaaagt gaaggcggcc ctggtggatc atgacaacat caagagcacc
      301 gatatctctg taaaaaccga tcaaaaagtc gtgaccctga gcggtttcgt tgaaagccag
      361 gcccaggccg aagaggcagt gaaagtggcg aaaggcgttg aaggggtgac ctctgtcagc
      421 gacaaactgc acgttcgcga cgctaaagaa ggctcggtga agggctacgc gggtgacacc
      481 gccaccacca gtgaaatcaa agccaaactg ctggcggacg atatcgtccc ttcccgtcat
      541 gtgaaagttg aaaccaccga cggcgtggtt cagctctccg gtaccgtcga ttctcaggca
      601 caaagtgacc gtgctgaaag tatcgccaaa gcggtagatg gtgtgaaaag cgttaaaaat
      661 gatctgaaaa ctaagtaata ctagagggtg gttctggtta ctagagcatc atcatcatca
      721 tcattactag aggaaaacct gtattttcag ggctactaga tggtgagcaa gggcgaggag
      781 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
      841 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
      901 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
      961 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     1021 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     1081 ggcgtggtga ccgtgaccca ggactcctcc ttgcaggacg gcgagttcat ctacaaggtg
     1141 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     1201 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     1261 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     1321 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     1381 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     1441 accggcggca tggacgagct gtacaagtta gtagcttaat aa
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