BBa_K157011
1
His
His affinity tag; Freiburg standard
2008-10-25T11:00:00Z
2015-05-08T01:10:54Z
Gene synthesis by ATG:biosynthetics, optimized for expression in homo sapiens by iGEM-Team Freiburg 2008
His-tag; fusion to proteins facilitates detection, purification, immobilization;
NgoMIV / AgeI protein fusion part.
false
true
_232_
0
1673
9
It's complicated
false
Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
true
Kristian M??ller
annotation2040626
1
His-Tag
range2040626
1
1
18
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_J06505
1
mCherry
monomeric RFP optimized for bacteria
2005-07-17T11:00:00Z
2015-08-31T04:08:18Z
mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible and with an LVA tag added.
Released HQ 2013
mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends.
false
false
_20_
0
340
20
In stock
false
<p>
Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends with the LVA tag using PCR.
</p>
<p>
Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.
</p>
false
ytwang
annotation1585879
1
mCherry
range1585879
1
1
708
annotation1585880
1
C->T (removing PstI site)
range1585880
1
352
352
annotation1585887
1
LVA
range1585887
1
709
717
BBa_K892008
1
osmY
osmY
2012-09-29T11:00:00Z
2015-05-08T01:13:41Z
E. coli genome
Osmotically induced protein Y shown to have exporting capabilities when fused to other proteins
false
false
_1156_
0
10541
9
In stock
true
None for now.
false
David Zong, Felix Ekness
annotation2207571
1
osmY
range2207571
1
1
606
BBa_K243004
1
ShortLinke
Short Linker (Gly-Gly-Ser-Gly)
2009-10-11T11:00:00Z
2015-05-08T01:11:37Z
Oligos synthesized by sigma. Hybridized by PCR.
This linker was used to connect two different parts. The sequence produced the aminoacids Gly-Gly-Ser-Gly.
false
true
_352_
0
4732
9
In stock
false
None.
false
Freiburg Bioware09
annotation2041881
1
Short Linker
range2041881
1
1
12
BBa_I712074
1
BBa_I712074
T7 promoter (strong promoter from T7 bacteriophage)
2007-10-21T11:00:00Z
2015-08-31T04:07:46Z
T7 bacteriophage
T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes.
false
false
_130_
0
1856
9
In stock
false
true
Rok Gaber
BBa_J18918
1
TEVsite
TEV cleavage site (E. coli)
2010-01-26T12:00:00Z
2015-08-31T04:08:36Z
gene synthesis
TEV cleaves the following AA sequence with high specificity:
* Glu-Asn-Leu-Tyr-Phe-Gln↓Gly
but:
* Glu-Asn-Leu-Tyr-Phe-Gln↓Ser
is also reported
Note, the part suggested by the Voigt lab, also contains 2 additional 1xGly flanks for increased accessibility.
See also:
* BBa_I712077 (TEV N-term)
* BBa_I712078 (TEV C-term)
* BBa_I712016 (myri+TEV site, Slovenia team igem2007)
* BBa_J64007 (Dan Widmaier, Voigt lab)
References
===========
Dougherty et al. (1989): Molecular genetic analysis of a plant virus polyprotein cleavage site: a model. PMID: 2669323
false
true
_165_
0
2175
165
It's complicated
false
* gene synthesis
* codon optimized for E. coli
false
Raik Gruenberg
BBa_K1519124
1
BBa_K1519124
MCherry fused to K1519123 for Characterization
2014-10-08T11:00:00Z
2015-05-08T01:10:48Z
MG1655 E. coli, Tsien Lab Mcherry
By inserting the DNA sequence of a desired protein in our part, one will be able to secret a protein of interest, bind it to a Nickel column, and cleave off the desired protein from the tag, resulting in a purified protein of interest. OsmY, the first gene in the device, has been shown to be a successful secretion tag. When fused to a protein of interest, the OsmY fusion translocates into the periplasm, where it forms disulfide bonds and folds. Through an unknown mechanism, the OsmY fusion is secreted out of the cell, into the growth medium. This secretion removes a significant amount of undesired protein contaminants.The media containing the construct can be run through a nickel column for further purification, utilizing the 6x His-tag incorporated into the construct. Once eluted from the column, addition of the TEV-His protease will cleave OsmY from the protein of interest, eliminating any unwanted effects of the device on the desired protein. Upon a second nickel column purification of the eluate, the TEV-His protease and the cleaved OsmY-His construct will remain on the column while the protein of interest runs off, resulting in an easily purified protein from the supernatant of the growth medium.
This construct is identical to K1519123, with an MCherry fusion for characterization
false
false
_1901_
0
18972
9
It's complicated
false
No design information provided.
false
Raoul Martin, Drew Dunham
component2408567
1
BBa_B0034
component2408569
1
BBa_K892008
component2408565
1
BBa_I712074
component2408574
1
BBa_J18918
component2408571
1
BBa_K243004
component2408573
1
BBa_K157011
component2408578
1
BBa_J06505
annotation2408569
1
BBa_K892008
range2408569
1
73
678
annotation2408578
1
BBa_J06505
range2408578
1
760
1482
annotation2408567
1
BBa_B0034
range2408567
1
55
66
annotation2408565
1
BBa_I712074
range2408565
1
1
46
annotation2408571
1
BBa_K243004
range2408571
1
687
698
annotation2408574
1
BBa_J18918
range2408574
1
733
753
annotation2408573
1
BBa_K157011
range2408573
1
707
724
BBa_K892008_sequence
1
atgactatgacaagactgaagatttcgaaaactctgctggctgtaatgttgacctctgccgtcgcgaccggctctgcctacgcggaaaacaacgcgcagactaccaatgaaagcgcagggcaaaaagtcgatagctctatgaataaagtcggtaatttcatggatgacagcgccatcaccgcgaaagtgaaggcggccctggtggatcatgacaacatcaagagcaccgatatctctgtaaaaaccgatcaaaaagtcgtgaccctgagcggtttcgttgaaagccaggcccaggccgaagaggcagtgaaagtggcgaaaggcgttgaaggggtgacctctgtcagcgacaaactgcacgttcgcgacgctaaagaaggctcggtgaagggctacgcgggtgacaccgccaccaccagtgaaatcaaagccaaactgctggcggacgatatcgtcccttcccgtcatgtgaaagttgaaaccaccgacggcgtggttcagctctccggtaccgtcgattctcaggcacaaagtgaccgtgctgaaagtatcgccaaagcggtagatggtgtgaaaagcgttaaaaatgatctgaaaactaagtaa
BBa_K243004_sequence
1
ggtggttctggt
BBa_J06505_sequence
1
atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagttagtagcttaataa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_I712074_sequence
1
taatacgactcactatagggaatacaagctacttgttctttttgca
BBa_K157011_sequence
1
catcatcatcatcatcat
BBa_J18918_sequence
1
gaaaacctgtattttcagggc
BBa_K1519124_sequence
1
taatacgactcactatagggaatacaagctacttgttctttttgcatactagagaaagaggagaaatactagatgactatgacaagactgaagatttcgaaaactctgctggctgtaatgttgacctctgccgtcgcgaccggctctgcctacgcggaaaacaacgcgcagactaccaatgaaagcgcagggcaaaaagtcgatagctctatgaataaagtcggtaatttcatggatgacagcgccatcaccgcgaaagtgaaggcggccctggtggatcatgacaacatcaagagcaccgatatctctgtaaaaaccgatcaaaaagtcgtgaccctgagcggtttcgttgaaagccaggcccaggccgaagaggcagtgaaagtggcgaaaggcgttgaaggggtgacctctgtcagcgacaaactgcacgttcgcgacgctaaagaaggctcggtgaagggctacgcgggtgacaccgccaccaccagtgaaatcaaagccaaactgctggcggacgatatcgtcccttcccgtcatgtgaaagttgaaaccaccgacggcgtggttcagctctccggtaccgtcgattctcaggcacaaagtgaccgtgctgaaagtatcgccaaagcggtagatggtgtgaaaagcgttaaaaatgatctgaaaactaagtaatactagagggtggttctggttactagagcatcatcatcatcatcattactagaggaaaacctgtattttcagggctactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagttagtagcttaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z