LOCUS BBa_K1520013 2408 bp DNA linear 22-Nov-2024 DEFINITION Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter ACCESSION BBa_K1520013 VERSION BBa_K1520013.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 misc_feature 64..795 /label=BBa_K1520500 RBS 804..821 /label=rbs CDS 822..1464 /label=luxI misc_feature 804..1464 /label=BBa_K1520504 RBS 1473..1490 /label=rbs CDS 1491..2271 /label=luxR misc_feature 1473..2271 /label=BBa_K1520501 terminator 2280..2408 /label=BBa_K1520029 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagaaagagg agaaattaag catggtgagc aagggcgagg aggataacat ggccatcatc 121 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag 181 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg 241 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc 301 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc 361 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc 421 caggactcct ccttgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac 481 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag 541 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag 601 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag 661 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac 721 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag 781 ctgtacaagt aataatacta gagaaagagg agaaattaag catgactata atgataaaaa 841 aatcggattt tttggcaatt ccatcggagg agtataaagg tattctaagt cttcgttatc 901 aagtgtttaa gcaaagactt gagtgggact tagttgtaga aaataacctt gaatcagatg 961 agtatgataa ctcaaatgca gaatatattt atgcttgtga tgatactgaa aatgtaagtg 1021 gatgctggcg tttattacct acaacaggtg attatatgct gaaaagtgtt tttcctgaat 1081 tgcttggtca acagagtgct cccaaagatc ctaatatagt cgaattaagt cgttttgctg 1141 taggtaaaaa tagctcaaag ataaataact ctgctagtga aattacaatg aaactatttg 1201 aagctatata taaacacgct gttagtcaag gtattacaga atatgtaaca gtaacatcaa 1261 cagcaataga gcgattttta aagcgtatta aagttccttg tcatcgtatt ggagacaaag 1321 aaattcatgt attaggtgat actaaatcgg ttgtattgtc tatgcctatt aatgaacagt 1381 ttaaaaaagc agtcttaaat gctgcaaacg acgaaaacta cgctttagta gcttaataac 1441 tctgatagtg ctagtgtaga tctctactag agaaagagga gaaattaagc atgaaaaaca 1501 taaatgccga cgacacatac agaataatta ataaaattaa agcttgtaga agcaataatg 1561 atattaatca atgcttatct gatatgacta aaatggtaca ttgtgaatat tatttactcg 1621 cgatcattta tcctcattct atggttaaat ctgatatttc aatcctagat aattacccta 1681 aaaaatggag gcaatattat gatgacgcta atttaataaa atatgatcct atagtagatt 1741 attctaactc caatcattca ccaattaatt ggaatatatt tgaaaacaat gctgtaaata 1801 aaaaatctcc aaatgtaatt aaagaagcga aaacatcagg tcttatcact gggtttagtt 1861 tccctattca tacggctaac aatggcttcg gaatgcttag ttttgcacat tcagaaaaag 1921 acaactatat agatagttta tttttacatg cgtgtatgaa cataccatta attgttcctt 1981 ctctagttga taattatcga aaaataaata tagcaaataa taaatcaaac aacgatttaa 2041 ccaaaagaga aaaagaatgt ttagcgtggg catgcgaagg aaaaagctct tgggatattt 2101 caaaaatatt aggttgcagt gagcgtactg tcactttcca tttaaccaat gcgcaaatga 2161 aactcaatac aacaaaccgc tgccaaagta tttctaaagc aattttaaca ggagcaattg 2221 attgcccata ctttaaaaat taataacact gatagtgcta gtgtagatca ctactagagc 2281 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 2341 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 2401 cgtttata //