LOCUS       BBa_K1520016            2358 bp    DNA     linear       14-Nov-2024
DEFINITION  Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter
ACCESSION   BBa_K1520016
VERSION     BBa_K1520016.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     RBS             44..61
                     /label=rbs
     CDS             62..842
                     /label=luxR
     misc_feature    44..842
                     /label=BBa_K1520501
     terminator      851..979
                     /label=BBa_K1520029
     misc_feature    1..979
                     /label=BBa_K1520003
     misc_feature    988..1007
                     /label=LuxR/HSL
     promoter        1007..1012
                     /label=-35
     promoter        1029..1034
                     /label=-10
     misc_feature    1040..1040
                     /label=start
     misc_feature    988..1042
                     /label=BBa_R0062
     promoter        988..1042
                     /label=BBa_R0062
     RBS             1051..1068
                     /label=rbs
     CDS             1069..2221
                     /label=lacI
     misc_feature    1051..2221
                     /label=BBa_K1520502
     terminator      2230..2358
                     /label=BBa_K1520029
     misc_feature    988..2358
                     /label=BBa_K1520004
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaattaag
       61 catgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag
      121 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata
      181 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga
      241 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc
      301 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa
      361 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac
      421 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca
      481 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt
      541 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa
      601 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc
      661 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa
      721 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac
      781 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc
      841 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      961 gggcctttct gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa
     1021 tggtttgtta tagtcgaata aatactagag aaagaggaga aattaagcat ggtgaatgtg
     1081 aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc
     1141 cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg
     1201 atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg
     1261 ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg
     1321 gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga
     1381 agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg
     1441 ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact
     1501 aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc
     1561 tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa
     1621 atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg
     1681 cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt
     1741 gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg
     1801 atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg
     1861 ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt
     1921 tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg
     1981 gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc
     2041 tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg
     2101 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcaggct
     2161 gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct
     2221 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     2281 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     2341 ggcctttctg cgtttata
//