LOCUS BBa_K1520017 3443 bp DNA linear 22-Nov-2024 DEFINITION Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter-Plac-rbs-rfp-Ter ACCESSION BBa_K1520017 VERSION BBa_K1520017.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 RBS 44..61 /label=rbs CDS 62..842 /label=luxR misc_feature 44..842 /label=BBa_K1520501 terminator 851..979 /label=BBa_K1520029 misc_feature 1..979 /label=BBa_K1520003 misc_feature 988..1007 /label=LuxR/HSL promoter 1007..1012 /label=-35 promoter 1029..1034 /label=-10 misc_feature 1040..1040 /label=start misc_feature 988..1042 /label=BBa_R0062 promoter 988..1042 /label=BBa_R0062 RBS 1051..1068 /label=rbs CDS 1069..2221 /label=lacI misc_feature 1051..2221 /label=BBa_K1520502 terminator 2230..2358 /label=BBa_K1520029 misc_feature 988..2358 /label=BBa_K1520004 misc_feature 1..2358 /label=BBa_K1520016 CDS 2367..2454 /label=end of LacI coding region (inactive) misc_feature 2367..2566 /label=BBa_R0010 misc_feature 2455..2492 /label=CAP binding site promoter 2503..2508 /label=-35 promoter 2527..2532 /label=-10 misc_feature 2532..2566 /label=LacI binding site misc_feature 2539..2539 /label=start promoter 2367..2566 /label=BBa_R0010 misc_feature 2575..3306 /label=BBa_K1520500 terminator 3315..3443 /label=BBa_K1520029 misc_feature 2367..3443 /label=BBa_K1520005 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaattaag 61 catgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag 121 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata 181 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga 241 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc 301 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa 361 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac 421 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca 481 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt 541 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa 601 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc 661 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa 721 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac 781 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc 841 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 961 gggcctttct gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa 1021 tggtttgtta tagtcgaata aatactagag aaagaggaga aattaagcat ggtgaatgtg 1081 aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc 1141 cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg 1201 atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg 1261 ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg 1321 gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga 1381 agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg 1441 ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact 1501 aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc 1561 tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa 1621 atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg 1681 cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt 1741 gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg 1801 atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg 1861 ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt 1921 tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg 1981 gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc 2041 tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg 2101 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcaggct 2161 gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct 2221 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 2281 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 2341 ggcctttctg cgtttatata ctagagcaat acgcaaaccg cctctccccg cgcgttggcc 2401 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 2461 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 2521 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacatact agagaaagag 2581 gagaaattaa gcatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 2641 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 2701 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 2761 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 2821 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 2881 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 2941 tccttgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 3001 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 3061 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 3121 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 3181 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 3241 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 3301 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 3361 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 3421 gggtgggcct ttctgcgttt ata //