LOCUS       BBa_K1520018            2366 bp    DNA     linear       14-Nov-2024
DEFINITION  Prlux-rbs-cI-Ter-PcI-rbs-lacI-Ter
ACCESSION   BBa_K1520018
VERSION     BBa_K1520018.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     RBS             64..81
                     /label=rbs
     CDS             82..856
                     /label=cI
     misc_feature    64..856
                     /label=BBa_K1520503
     terminator      865..993
                     /label=BBa_K1520029
     misc_feature    1..993
                     /label=BBa_K1520006
     promoter        1039..1044
                     /label=-10
     promoter        1016..1021
                     /label=-35
     misc_feature    1026..1042
                     /label=OR1
     misc_feature    1002..1018
                     /label=OR2
     misc_feature    1002..1050
                     /label=BBa_R0051
     promoter        1002..1050
                     /label=BBa_R0051
     RBS             1059..1076
                     /label=rbs
     CDS             1077..2229
                     /label=lacI
     misc_feature    1059..2229
                     /label=BBa_K1520502
     terminator      2238..2366
                     /label=BBa_K1520029
     misc_feature    1002..2366
                     /label=BBa_K1520007
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaaagagg agaaattaag catgagcaca aaaaagaaac cattaacaca agagcagctt
      121 gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttatcc
      181 caggaatctg tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tttatttaat
      241 ggcatcaatg cattaaatgc ttataacgcc gcattgcttg caaaaattct caaagttagc
      301 gttgaagaat ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt
      361 atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg
      421 atgttctcac ctgagcttag aacctttacc aaaggtgatg cggagagatg ggtaagcaca
      481 accaaaaaag ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca
      541 ccaacaggct ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag
      601 gctgttgagc caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag
      661 aaactgatca gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg
      721 atcccatgca atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa
      781 gagacgtttg gcgctgcaaa cgacgaaaac tacgctttag tagcttaata acgctgatag
      841 tgctagtgta gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
      901 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
      961 gctcaccttc gggtgggcct ttctgcgttt atatactaga gtaacaccgt gcgtgttgac
     1021 tattttacct ctggcggtga taatggttgc tactagagaa agaggagaaa ttaagcatgg
     1081 tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga
     1141 ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg
     1201 aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca
     1261 aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa
     1321 ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg
     1381 tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg
     1441 tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg
     1501 cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta
     1561 ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc
     1621 accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg
     1681 ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg
     1741 actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc
     1801 ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg
     1861 agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca
     1921 gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa
     1981 ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt
     2041 tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc
     2101 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     2161 ggcaggctgc aaacgacgaa aactacgctt tagtagctta ataactctga tagtgctagt
     2221 gtagatctct actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     2281 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     2341 ttcgggtggg cctttctgcg tttata
//