Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter It produces luxI when there is enough IPTG.
Pcons2-rbs-lacI-Ter. It constantly produces lacI.
Plac-rbs-luxI-Ter. It produces luxI when there is enough IPTG or less lacI.
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
It is an improvement of lacI by adding a rbs.
Rbs-lacI(scarless) It is an improvement of lacI by adding a rbs.
It is an improvement of luxI(BBa_C0061) by adding a rbs.
Rbs-luxI(scarless) It is an improvement of luxI(BBa_C0061) by adding a rbs.
Conversion of the iGEM parts registry to SBOL2.1