LOCUS       BBa_K1520020            3352 bp    DNA     linear       07-May-2024
DEFINITION  Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter
ACCESSION   BBa_K1520020
VERSION     BBa_K1520020.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     RBS             44..61
                     /label=rbs
     CDS             62..1214
                     /label=lacI
     misc_feature    44..1214
                     /label=BBa_K1520502
     terminator      1223..1351
                     /label=BBa_K1520029
     misc_feature    1..1351
                     /label=BBa_K1520008
     CDS             1360..1447
                     /label=end of LacI coding region (inactive)
     misc_feature    1360..1559
                     /label=BBa_R0010
     misc_feature    1448..1485
                     /label=CAP binding site
     promoter        1496..1501
                     /label=-35
     promoter        1520..1525
                     /label=-10
     misc_feature    1525..1559
                     /label=LacI binding site
     misc_feature    1532..1532
                     /label=start
     promoter        1360..1559
                     /label=BBa_R0010
     RBS             1568..1585
                     /label=rbs
     CDS             1586..2228
                     /label=luxI
     misc_feature    1568..2228
                     /label=BBa_K1520504
     terminator      2237..2365
                     /label=BBa_K1520029
     misc_feature    1360..2365
                     /label=BBa_K1520009
     misc_feature    1..2365
                     /label=BBa_K1520019
     promoter        2374..2408
                     /label=BBa_J23100
     RBS             2417..2434
                     /label=rbs
     CDS             2435..3215
                     /label=luxR
     misc_feature    2417..3215
                     /label=BBa_K1520501
     terminator      3224..3352
                     /label=BBa_K1520029
     misc_feature    2374..3352
                     /label=BBa_K1520003
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaattaag
       61 catggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta
      121 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa
      181 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
      241 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
      301 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
      361 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
      421 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga
      481 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa
      541 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt
      601 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg
      661 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga
      721 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat
      781 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat
      841 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga
      901 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg
      961 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
     1021 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc
     1081 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     1141 aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg
     1201 ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     1261 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1321 tcaccttcgg gtgggccttt ctgcgtttat atactagagc aatacgcaaa ccgcctctcc
     1381 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     1441 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     1501 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacat
     1561 actagagaaa gaggagaaat taagcatgac tataatgata aaaaaatcgg attttttggc
     1621 aattccatcg gaggagtata aaggtattct aagtcttcgt tatcaagtgt ttaagcaaag
     1681 acttgagtgg gacttagttg tagaaaataa ccttgaatca gatgagtatg ataactcaaa
     1741 tgcagaatat atttatgctt gtgatgatac tgaaaatgta agtggatgct ggcgtttatt
     1801 acctacaaca ggtgattata tgctgaaaag tgtttttcct gaattgcttg gtcaacagag
     1861 tgctcccaaa gatcctaata tagtcgaatt aagtcgtttt gctgtaggta aaaatagctc
     1921 aaagataaat aactctgcta gtgaaattac aatgaaacta tttgaagcta tatataaaca
     1981 cgctgttagt caaggtatta cagaatatgt aacagtaaca tcaacagcaa tagagcgatt
     2041 tttaaagcgt attaaagttc cttgtcatcg tattggagac aaagaaattc atgtattagg
     2101 tgatactaaa tcggttgtat tgtctatgcc tattaatgaa cagtttaaaa aagcagtctt
     2161 aaatgctgca aacgacgaaa actacgcttt agtagcttaa taactctgat agtgctagtg
     2221 tagatctcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     2281 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     2341 tcgggtgggc ctttctgcgt ttatatacta gagttgacgg ctagctcagt cctaggtaca
     2401 gtgctagcta ctagagaaag aggagaaatt aagcatgaaa aacataaatg ccgacgacac
     2461 atacagaata attaataaaa ttaaagcttg tagaagcaat aatgatatta atcaatgctt
     2521 atctgatatg actaaaatgg tacattgtga atattattta ctcgcgatca tttatcctca
     2581 ttctatggtt aaatctgata tttcaatcct agataattac cctaaaaaat ggaggcaata
     2641 ttatgatgac gctaatttaa taaaatatga tcctatagta gattattcta actccaatca
     2701 ttcaccaatt aattggaata tatttgaaaa caatgctgta aataaaaaat ctccaaatgt
     2761 aattaaagaa gcgaaaacat caggtcttat cactgggttt agtttcccta ttcatacggc
     2821 taacaatggc ttcggaatgc ttagttttgc acattcagaa aaagacaact atatagatag
     2881 tttattttta catgcgtgta tgaacatacc attaattgtt ccttctctag ttgataatta
     2941 tcgaaaaata aatatagcaa ataataaatc aaacaacgat ttaaccaaaa gagaaaaaga
     3001 atgtttagcg tgggcatgcg aaggaaaaag ctcttgggat atttcaaaaa tattaggttg
     3061 cagtgagcgt actgtcactt tccatttaac caatgcgcaa atgaaactca atacaacaaa
     3121 ccgctgccaa agtatttcta aagcaatttt aacaggagca attgattgcc catactttaa
     3181 aaattaataa cactgatagt gctagtgtag atcactacta gagccaggca tcaaataaaa
     3241 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     3301 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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