LOCUS BBa_K1520021 4292 bp DNA linear 22-Dec-2024 DEFINITION Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs -rfp-Ter ACCESSION BBa_K1520021 VERSION BBa_K1520021.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 RBS 44..61 /label=rbs CDS 62..1214 /label=lacI misc_feature 44..1214 /label=BBa_K1520502 terminator 1223..1351 /label=BBa_K1520029 misc_feature 1..1351 /label=BBa_K1520008 CDS 1360..1447 /label=end of LacI coding region (inactive) misc_feature 1360..1559 /label=BBa_R0010 misc_feature 1448..1485 /label=CAP binding site promoter 1496..1501 /label=-35 promoter 1520..1525 /label=-10 misc_feature 1525..1559 /label=LacI binding site misc_feature 1532..1532 /label=start promoter 1360..1559 /label=BBa_R0010 RBS 1568..1585 /label=rbs CDS 1586..2228 /label=luxI misc_feature 1568..2228 /label=BBa_K1520504 terminator 2237..2365 /label=BBa_K1520029 misc_feature 1360..2365 /label=BBa_K1520009 misc_feature 1..2365 /label=BBa_K1520019 promoter 2374..2408 /label=BBa_J23100 RBS 2417..2434 /label=rbs CDS 2435..3215 /label=luxR misc_feature 2417..3215 /label=BBa_K1520501 terminator 3224..3352 /label=BBa_K1520029 misc_feature 2374..3352 /label=BBa_K1520003 misc_feature 1..3352 /label=BBa_K1520020 misc_feature 3361..3380 /label=LuxR/HSL promoter 3380..3385 /label=-35 promoter 3402..3407 /label=-10 misc_feature 3413..3413 /label=start misc_feature 3361..3415 /label=BBa_R0062 promoter 3361..3415 /label=BBa_R0062 misc_feature 3424..4155 /label=BBa_K1520500 terminator 4164..4292 /label=BBa_K1520029 misc_feature 3361..4292 /label=BBa_K1520010 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaattaag 61 catggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta 121 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa 181 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc 241 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc 301 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc 361 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca 421 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga 481 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa 541 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt 601 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg 661 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga 721 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat 781 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat 841 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga 901 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg 961 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca 1021 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc 1081 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 1141 aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg 1201 ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 1261 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 1321 tcaccttcgg gtgggccttt ctgcgtttat atactagagc aatacgcaaa ccgcctctcc 1381 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 1441 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 1501 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacat 1561 actagagaaa gaggagaaat taagcatgac tataatgata aaaaaatcgg attttttggc 1621 aattccatcg gaggagtata aaggtattct aagtcttcgt tatcaagtgt ttaagcaaag 1681 acttgagtgg gacttagttg tagaaaataa ccttgaatca gatgagtatg ataactcaaa 1741 tgcagaatat atttatgctt gtgatgatac tgaaaatgta agtggatgct ggcgtttatt 1801 acctacaaca ggtgattata tgctgaaaag tgtttttcct gaattgcttg gtcaacagag 1861 tgctcccaaa gatcctaata tagtcgaatt aagtcgtttt gctgtaggta aaaatagctc 1921 aaagataaat aactctgcta gtgaaattac aatgaaacta tttgaagcta tatataaaca 1981 cgctgttagt caaggtatta cagaatatgt aacagtaaca tcaacagcaa tagagcgatt 2041 tttaaagcgt attaaagttc cttgtcatcg tattggagac aaagaaattc atgtattagg 2101 tgatactaaa tcggttgtat tgtctatgcc tattaatgaa cagtttaaaa aagcagtctt 2161 aaatgctgca aacgacgaaa actacgcttt agtagcttaa taactctgat agtgctagtg 2221 tagatctcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 2281 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 2341 tcgggtgggc ctttctgcgt ttatatacta gagttgacgg ctagctcagt cctaggtaca 2401 gtgctagcta ctagagaaag aggagaaatt aagcatgaaa aacataaatg ccgacgacac 2461 atacagaata attaataaaa ttaaagcttg tagaagcaat aatgatatta atcaatgctt 2521 atctgatatg actaaaatgg tacattgtga atattattta ctcgcgatca tttatcctca 2581 ttctatggtt aaatctgata tttcaatcct agataattac cctaaaaaat ggaggcaata 2641 ttatgatgac gctaatttaa taaaatatga tcctatagta gattattcta actccaatca 2701 ttcaccaatt aattggaata tatttgaaaa caatgctgta aataaaaaat ctccaaatgt 2761 aattaaagaa gcgaaaacat caggtcttat cactgggttt agtttcccta ttcatacggc 2821 taacaatggc ttcggaatgc ttagttttgc acattcagaa aaagacaact atatagatag 2881 tttattttta catgcgtgta tgaacatacc attaattgtt ccttctctag ttgataatta 2941 tcgaaaaata aatatagcaa ataataaatc aaacaacgat ttaaccaaaa gagaaaaaga 3001 atgtttagcg tgggcatgcg aaggaaaaag ctcttgggat atttcaaaaa tattaggttg 3061 cagtgagcgt actgtcactt tccatttaac caatgcgcaa atgaaactca atacaacaaa 3121 ccgctgccaa agtatttcta aagcaatttt aacaggagca attgattgcc catactttaa 3181 aaattaataa cactgatagt gctagtgtag atcactacta gagccaggca tcaaataaaa 3241 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 3301 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag 3361 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 3421 gagaaagagg agaaattaag catggtgagc aagggcgagg aggataacat ggccatcatc 3481 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag 3541 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg 3601 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc 3661 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc 3721 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc 3781 caggactcct ccttgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac 3841 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag 3901 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag 3961 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag 4021 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac 4081 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag 4141 ctgtacaagt aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 4201 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 4261 ctcaccttcg ggtgggcctt tctgcgttta ta //