##gff-version 3
##sequence-region BBa_K1520022 1 4961
BBa_K1520022	.	engineered_region	1	3352	.	+	0	ID=BBa_K1520020;Name=BBa_K1520020
BBa_K1520022	.	engineered_region	2374	3352	.	+	0	ID=BBa_K1520003;Name=BBa_K1520003;Parent=BBa_K1520020
BBa_K1520022	.	terminator	3224	3352	.	+	0	ID=BBa_K1520029;Name=BBa_K1520029;Parent=BBa_K1520003
BBa_K1520022	.	promoter	2374	2408	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K1520003
BBa_K1520022	.	engineered_region	2417	3215	.	+	0	ID=BBa_K1520501;Name=BBa_K1520501;Parent=BBa_K1520003
BBa_K1520022	.	CDS	2435	3215	.	+	0	ID=annotation2384862;Name=luxR;Parent=BBa_K1520501
BBa_K1520022	.	ribosome_entry_site	2417	2434	.	+	0	ID=annotation2384861;Name=rbs;Parent=BBa_K1520501
BBa_K1520022	.	engineered_region	1	2365	.	+	0	ID=BBa_K1520019;Name=BBa_K1520019;Parent=BBa_K1520020
BBa_K1520022	.	engineered_region	1	1351	.	+	0	ID=BBa_K1520008;Name=BBa_K1520008;Parent=BBa_K1520019
BBa_K1520022	.	terminator	1223	1351	.	+	0	ID=BBa_K1520029;Name=BBa_K1520029;Parent=BBa_K1520008
BBa_K1520022	.	engineered_region	44	1214	.	+	0	ID=BBa_K1520502;Name=BBa_K1520502;Parent=BBa_K1520008
BBa_K1520022	.	CDS	62	1214	.	+	0	ID=annotation2384864;Name=lacI;Parent=BBa_K1520502
BBa_K1520022	.	ribosome_entry_site	44	61	.	+	0	ID=annotation2384863;Name=rbs;Parent=BBa_K1520502
BBa_K1520022	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K1520008
BBa_K1520022	.	engineered_region	1360	2365	.	+	0	ID=BBa_K1520009;Name=BBa_K1520009;Parent=BBa_K1520019
BBa_K1520022	.	terminator	2237	2365	.	+	0	ID=BBa_K1520029;Name=BBa_K1520029;Parent=BBa_K1520009
BBa_K1520022	.	promoter	1360	1559	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K1520009
BBa_K1520022	.	CDS	1360	1447	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1520022	.	promoter	1496	1501	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1520022	.	engineered_region	1360	1559	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1520022	.	non_covalent_binding_site	1448	1485	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1520022	.	promoter	1520	1525	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1520022	.	start_codon	1532	1532	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1520022	.	non_covalent_binding_site	1525	1559	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1520022	.	engineered_region	1568	2228	.	+	0	ID=BBa_K1520504;Name=BBa_K1520504;Parent=BBa_K1520009
BBa_K1520022	.	ribosome_entry_site	1568	1585	.	+	0	ID=annotation2384869;Name=rbs;Parent=BBa_K1520504
BBa_K1520022	.	CDS	1586	2228	.	+	0	ID=annotation2384870;Name=luxI;Parent=BBa_K1520504
BBa_K1520022	.	engineered_region	3361	4961	.	+	0	ID=BBa_K1520011;Name=BBa_K1520011
BBa_K1520022	.	engineered_region	3424	4155	.	+	0	ID=BBa_K1520500;Name=BBa_K1520500;Parent=BBa_K1520011
BBa_K1520022	.	terminator	4833	4961	.	+	0	ID=BBa_K1520029;Name=BBa_K1520029;Parent=BBa_K1520011
BBa_K1520022	.	promoter	3361	3415	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_K1520011
BBa_K1520022	.	non_covalent_binding_site	3361	3380	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1520022	.	promoter	3402	3407	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1520022	.	start_codon	3413	3413	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1520022	.	promoter	3380	3385	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1520022	.	engineered_region	3361	3415	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1520022	.	engineered_region	4164	4824	.	+	0	ID=BBa_K1520504;Name=BBa_K1520504;Parent=BBa_K1520011
BBa_K1520022	.	ribosome_entry_site	4164	4181	.	+	0	ID=annotation2384869;Name=rbs;Parent=BBa_K1520504
BBa_K1520022	.	CDS	4182	4824	.	+	0	ID=annotation2384870;Name=luxI;Parent=BBa_K1520504
>BBa_K1520022
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaattaagcatggtgaatgtgaaacca
gtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacg
tttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaact
ggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcg
attaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaag
cggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgc
tgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttc
tcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcc
cattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagc
ggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccact
gcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcgg
atatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattt
tcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttg
cccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcat
taatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagctta
ataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggt
ttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttaca
ctttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaat
taagcatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcg
ttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactca
aatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgatt
atatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcg
ttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatat
aaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtatta
aagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctat
taatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatag
tgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttat
ctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatacta
gagttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaattaagcatgaaaaacataaat
gccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgata
tgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaat
cctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattat
tctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaatta
aagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttag
ttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttcct
tctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaat
gtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcacttt
ccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagca
attgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggt
ttgttatagtcgaataaatactagagaaagaggagaaattaagcatggtgagcaagggcgaggaggataacatggccat
catcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggc
gagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggaca
tcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtc
cttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctcc
ttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaaga
agaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggct
gaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggc
gcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccg
agggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagaaagaggagaaattaagcatgact
ataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgt
ttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaata
tatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaa
agtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtag
gtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgt
tagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgt
catcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagt
ttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtag
atctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgt
cggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata