BBa_K1520023
type: ComponentDefinition
persistent identity: BBa K1520023
display id: BBa_K1520023
version: 1
was generated by: iGEM to SBOL conversion
description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxR-Ter
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
mutable provenance: Pcons2, lacI, Plac, luxI, luxR, Prlux, rfp, Ter
owned by: james
owned by: myers
mutable description: Plasmid in positive feedback verification: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxR-Ter. It is a positive feedback plasmid using luxR as key to feedback. Introducing IPTG will produce rfp and luxR, and luxR will work back to enhance production of rfp.
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: yue wu
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1520012
component: BBa_K1520020
sequence annotation: BBa_K1520020
sequence annotation: BBa_K1520012
sequence: https://synbiohub.org/public/igem/BBa_K1520023_sequence/1

BBa_K1520012
type: Component
persistent identity: component2385224
display id: component2385224
version: 1
top level: BBa_K1520023
definition: BBa_K1520012
access: public

BBa_K1520020
type: Component
persistent identity: component2385213
display id: component2385213
version: 1
top level: BBa_K1520023
definition: BBa_K1520020
access: public

BBa_K1520020
type: SequenceAnnotation
persistent identity: annotation2385213
display id: annotation2385213
version: 1
top level: BBa_K1520023
location: https://synbiohub.org/public/igem/BBa_K1520023/annotation2385213/range2385213/1
component: BBa_K1520020

https://synbiohub.org/public/igem/BBa_K1520023/annotation2385213/range2385213/1
type: Range
persistent identity: range2385213
display id: range2385213
version: 1
top level: BBa_K1520023
direction: direction/reverse
start: 1
end: 3352
orientation: inline

BBa_K1520012
type: SequenceAnnotation
persistent identity: annotation2385224
display id: annotation2385224
version: 1
top level: BBa_K1520023
location: https://synbiohub.org/public/igem/BBa_K1520023/annotation2385224/range2385224/1
component: BBa_K1520012

https://synbiohub.org/public/igem/BBa_K1520023/annotation2385224/range2385224/1
type: Range
persistent identity: range2385224
display id: range2385224
version: 1
top level: BBa_K1520023
direction: direction/reverse
start: 3361
end: 5099
orientation: inline

BBa_K1520012
type: ComponentDefinition
persistent identity: BBa K1520012
display id: BBa_K1520012
version: 1
was generated by: iGEM to SBOL conversion
description: Prlux-rbs-rfp-rbs-luxR-Ter
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
mutable provenance: Prlux, rfp, luxR, Ter
owned by: james
owned by: myers
mutable description: Prlux-rbs-rfp-rbs-luxR-Ter. It produces rfp and luxR when there is enough rfp and luxR.
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: yue wu
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0062
component: BBa_K1520029
component: BBa_K1520501
component: BBa_K1520500
sequence annotation: BBa_R0062
sequence annotation: BBa_K1520500
sequence annotation: BBa_K1520501
sequence annotation: BBa_K1520029
sequence: https://synbiohub.org/public/igem/BBa_K1520012_sequence/1

BBa_R0062
type: Component
persistent identity: component2384968
display id: component2384968
version: 1
top level: BBa_K1520012
definition: lux pR
access: public

BBa_K1520029
type: Component
persistent identity: component2384977
display id: component2384977
version: 1
top level: BBa_K1520012
definition: BBa_K1520029
access: public

BBa_K1520501
type: Component
persistent identity: component2384976
display id: component2384976
version: 1
top level: BBa_K1520012
definition: BBa_K1520501
access: public

BBa_K1520500
type: Component
persistent identity: component2384973
display id: component2384973
version: 1
top level: BBa_K1520012
definition: BBa_K1520500
access: public

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2384968
display id: annotation2384968
version: 1
top level: BBa_K1520012
location: https://synbiohub.org/public/igem/BBa_K1520012/annotation2384968/range2384968/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1520012/annotation2384968/range2384968/1
type: Range
persistent identity: range2384968
display id: range2384968
version: 1
top level: BBa_K1520012
direction: direction/forward
start: 1
end: 55
orientation: inline

BBa_K1520500
type: SequenceAnnotation
persistent identity: annotation2384973
display id: annotation2384973
version: 1
top level: BBa_K1520012
location: https://synbiohub.org/public/igem/BBa_K1520012/annotation2384973/range2384973/1
component: BBa_K1520500

https://synbiohub.org/public/igem/BBa_K1520012/annotation2384973/range2384973/1
type: Range
persistent identity: range2384973
display id: range2384973
version: 1
top level: BBa_K1520012
direction: direction/reverse
start: 64
end: 795
orientation: inline

BBa_K1520501
type: SequenceAnnotation
persistent identity: annotation2384976
display id: annotation2384976
version: 1
top level: BBa_K1520012
location: https://synbiohub.org/public/igem/BBa_K1520012/annotation2384976/range2384976/1
component: BBa_K1520501

https://synbiohub.org/public/igem/BBa_K1520012/annotation2384976/range2384976/1
type: Range
persistent identity: range2384976
display id: range2384976
version: 1
top level: BBa_K1520012
direction: direction/reverse
start: 804
end: 1602
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384977
display id: annotation2384977
version: 1
top level: BBa_K1520012
location: https://synbiohub.org/public/igem/BBa_K1520012/annotation2384977/range2384977/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520012/annotation2384977/range2384977/1
type: Range
persistent identity: range2384977
display id: range2384977
version: 1
top level: BBa_K1520012
direction: direction/reverse
start: 1611
end: 1739
orientation: inline

BBa_K1520020
type: ComponentDefinition
persistent identity: BBa K1520020
display id: BBa_K1520020
version: 1
was generated by: iGEM to SBOL conversion
description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
mutable provenance: Pcons2, lacI, Plac, luxI, Ter
owned by: james
owned by: myers
mutable description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter It produces luxI when there is enough IPTG.
discontinued: false
dominant: false
experience: experience/None
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: yue wu
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1520003
component: BBa_K1520019
sequence annotation: BBa_K1520019
sequence annotation: BBa_K1520003
sequence: https://synbiohub.org/public/igem/BBa_K1520020_sequence/1

BBa_K1520003
type: Component
persistent identity: component2385116
display id: component2385116
version: 1
top level: BBa_K1520020
definition: BBa_K1520003
access: public

BBa_K1520019
type: Component
persistent identity: component2385110
display id: component2385110
version: 1
top level: BBa_K1520020
definition: BBa_K1520019
access: public

BBa_K1520019
type: SequenceAnnotation
persistent identity: annotation2385110
display id: annotation2385110
version: 1
top level: BBa_K1520020
location: https://synbiohub.org/public/igem/BBa_K1520020/annotation2385110/range2385110/1
component: BBa_K1520019

https://synbiohub.org/public/igem/BBa_K1520020/annotation2385110/range2385110/1
type: Range
persistent identity: range2385110
display id: range2385110
version: 1
top level: BBa_K1520020
direction: direction/reverse
start: 1
end: 2365
orientation: inline

BBa_K1520003
type: SequenceAnnotation
persistent identity: annotation2385116
display id: annotation2385116
version: 1
top level: BBa_K1520020
location: https://synbiohub.org/public/igem/BBa_K1520020/annotation2385116/range2385116/1
component: BBa_K1520003

https://synbiohub.org/public/igem/BBa_K1520020/annotation2385116/range2385116/1
type: Range
persistent identity: range2385116
display id: range2385116
version: 1
top level: BBa_K1520020
direction: direction/reverse
start: 2374
end: 3352
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (luxR & HSL regulated -- lux pR)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: V. fischeri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0062
sequence annotation: LuxR/HSL
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 55
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
top level: lux pR
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
top level: lux pR
direction: direction/forward
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
top level: lux pR
direction: direction/forward
start: 53
end: 53
orientation: inline

BBa_K1520029
type: ComponentDefinition
persistent identity: BBa K1520029
display id: BBa_K1520029
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
mutable provenance: It's designed.
owned by: james
owned by: myers
mutable description: It's a terminator with two hairpin.
discontinued: false
dominant: false
experience: experience/None
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: None
star: false
creator: Peiqing Sun
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_K1520029_sequence/1

BBa_K1520500
type: ComponentDefinition
persistent identity: BBa K1520500
display id: BBa_K1520500
version: 1
was generated by: iGEM to SBOL conversion
description: red fluorescent protein
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
mutable provenance: NONE
owned by: james
owned by: myers
mutable description: red fluorescent protein
discontinued: false
dominant: false
experience: experience/None
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: NONE
star: false
creator: Peiqing Sun
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence: https://synbiohub.org/public/igem/BBa_K1520500_sequence/1

BBa_K1520003
type: ComponentDefinition
persistent identity: BBa K1520003
display id: BBa_K1520003
version: 1
was generated by: iGEM to SBOL conversion
description: Pcons2-rbs-luxR-Ter
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:48Z
mutable provenance: Pcons2, luxR, Ter
owned by: james
owned by: myers
mutable description: Pcons2-rbs-luxR-Ter. It achieves a constant expression of luxR, the AHL combining protein Pcons2 is BBa_J23100
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: NONE
star: false
creator: yue wu
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_K1520029
component: BBa_K1520501
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_K1520501
sequence annotation: BBa_K1520029
sequence: https://synbiohub.org/public/igem/BBa_K1520003_sequence/1

BBa_K1520029
type: Component
persistent identity: component2384888
display id: component2384888
version: 1
top level: BBa_K1520003
definition: BBa_K1520029
access: public

BBa_K1520501
type: Component
persistent identity: component2384887
display id: component2384887
version: 1
top level: BBa_K1520003
definition: BBa_K1520501
access: public

BBa_J23100
type: Component
persistent identity: component2384884
display id: component2384884
version: 1
top level: BBa_K1520003
definition: BBa_J23100
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2384884
display id: annotation2384884
version: 1
top level: BBa_K1520003
location: https://synbiohub.org/public/igem/BBa_K1520003/annotation2384884/range2384884/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1520003/annotation2384884/range2384884/1
type: Range
persistent identity: range2384884
display id: range2384884
version: 1
top level: BBa_K1520003
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_K1520501
type: SequenceAnnotation
persistent identity: annotation2384887
display id: annotation2384887
version: 1
top level: BBa_K1520003
location: https://synbiohub.org/public/igem/BBa_K1520003/annotation2384887/range2384887/1
component: BBa_K1520501

https://synbiohub.org/public/igem/BBa_K1520003/annotation2384887/range2384887/1
type: Range
persistent identity: range2384887
display id: range2384887
version: 1
top level: BBa_K1520003
direction: direction/reverse
start: 44
end: 842
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384888
display id: annotation2384888
version: 1
top level: BBa_K1520003
location: https://synbiohub.org/public/igem/BBa_K1520003/annotation2384888/range2384888/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520003/annotation2384888/range2384888/1
type: Range
persistent identity: range2384888
display id: range2384888
version: 1
top level: BBa_K1520003
direction: direction/reverse
start: 851
end: 979
orientation: inline

BBa_K1520501
type: ComponentDefinition
persistent identity: BBa K1520501
display id: BBa_K1520501
version: 1
was generated by: iGEM to SBOL conversion
description: Rbs-luxR(scarless)
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
mutable provenance: None
owned by: james
owned by: myers
mutable description: Rbs-luxR(scarless)
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: yue wu
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: rbs
sequence annotation: luxR
sequence: https://synbiohub.org/public/igem/BBa_K1520501_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2384861
display id: annotation2384861
version: 1
top level: BBa_K1520501
location: https://synbiohub.org/public/igem/BBa_K1520501/annotation2384861/range2384861/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1520501/annotation2384861/range2384861/1
type: Range
persistent identity: range2384861
display id: range2384861
version: 1
top level: BBa_K1520501
direction: direction/forward
start: 1
end: 18
orientation: inline

luxR
type: SequenceAnnotation
persistent identity: annotation2384862
display id: annotation2384862
version: 1
top level: BBa_K1520501
location: https://synbiohub.org/public/igem/BBa_K1520501/annotation2384862/range2384862/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1520501/annotation2384862/range2384862/1
type: Range
persistent identity: range2384862
display id: range2384862
version: 1
top level: BBa_K1520501
direction: direction/forward
start: 19
end: 799
orientation: inline

BBa_K1520019
type: ComponentDefinition
persistent identity: BBa K1520019
display id: BBa_K1520019
version: 1
was generated by: iGEM to SBOL conversion
description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
mutable provenance: Pcons2, lacI, Plac, luxI, Ter
owned by: james
owned by: myers
mutable description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter It produces luxI when there is enough IPTG.
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: yue wu
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520009
component: BBa_K1520008
sequence annotation: BBa_K1520008
sequence annotation: BBa_K1520009
sequence: https://synbiohub.org/public/igem/BBa_K1520019_sequence/1

BBa_K1520009
type: Component
persistent identity: component2385091
display id: component2385091
version: 1
top level: BBa_K1520019
definition: BBa_K1520009
access: public

BBa_K1520008
type: Component
persistent identity: component2385079
display id: component2385079
version: 1
top level: BBa_K1520019
definition: BBa_K1520008
access: public

BBa_K1520008
type: SequenceAnnotation
persistent identity: annotation2385079
display id: annotation2385079
version: 1
top level: BBa_K1520019
location: https://synbiohub.org/public/igem/BBa_K1520019/annotation2385079/range2385079/1
component: BBa_K1520008

https://synbiohub.org/public/igem/BBa_K1520019/annotation2385079/range2385079/1
type: Range
persistent identity: range2385079
display id: range2385079
version: 1
top level: BBa_K1520019
direction: direction/reverse
start: 1
end: 1351
orientation: inline

BBa_K1520009
type: SequenceAnnotation
persistent identity: annotation2385091
display id: annotation2385091
version: 1
top level: BBa_K1520019
location: https://synbiohub.org/public/igem/BBa_K1520019/annotation2385091/range2385091/1
component: BBa_K1520009

https://synbiohub.org/public/igem/BBa_K1520019/annotation2385091/range2385091/1
type: Range
persistent identity: range2385091
display id: range2385091
version: 1
top level: BBa_K1520019
direction: direction/reverse
start: 1360
end: 2365
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

BBa_K1520008
type: ComponentDefinition
persistent identity: BBa K1520008
display id: BBa_K1520008
version: 1
was generated by: iGEM to SBOL conversion
description: Pcons2-rbs-lacI-Ter
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:48Z
mutable provenance: Pcons2, lacI, Ter
owned by: james
owned by: myers
mutable description: Pcons2-rbs-lacI-Ter. It constantly produces lacI.
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: yue wu
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_K1520029
component: BBa_K1520502
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_K1520502
sequence annotation: BBa_K1520029
sequence: https://synbiohub.org/public/igem/BBa_K1520008_sequence/1

BBa_K1520029
type: Component
persistent identity: component2384936
display id: component2384936
version: 1
top level: BBa_K1520008
definition: BBa_K1520029
access: public

BBa_K1520502
type: Component
persistent identity: component2384935
display id: component2384935
version: 1
top level: BBa_K1520008
definition: BBa_K1520502
access: public

BBa_J23100
type: Component
persistent identity: component2384932
display id: component2384932
version: 1
top level: BBa_K1520008
definition: BBa_J23100
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2384932
display id: annotation2384932
version: 1
top level: BBa_K1520008
location: https://synbiohub.org/public/igem/BBa_K1520008/annotation2384932/range2384932/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1520008/annotation2384932/range2384932/1
type: Range
persistent identity: range2384932
display id: range2384932
version: 1
top level: BBa_K1520008
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_K1520502
type: SequenceAnnotation
persistent identity: annotation2384935
display id: annotation2384935
version: 1
top level: BBa_K1520008
location: https://synbiohub.org/public/igem/BBa_K1520008/annotation2384935/range2384935/1
component: BBa_K1520502

https://synbiohub.org/public/igem/BBa_K1520008/annotation2384935/range2384935/1
type: Range
persistent identity: range2384935
display id: range2384935
version: 1
top level: BBa_K1520008
direction: direction/reverse
start: 44
end: 1214
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384936
display id: annotation2384936
version: 1
top level: BBa_K1520008
location: https://synbiohub.org/public/igem/BBa_K1520008/annotation2384936/range2384936/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520008/annotation2384936/range2384936/1
type: Range
persistent identity: range2384936
display id: range2384936
version: 1
top level: BBa_K1520008
direction: direction/reverse
start: 1223
end: 1351
orientation: inline

BBa_K1520009
type: ComponentDefinition
persistent identity: BBa K1520009
display id: BBa_K1520009
version: 1
was generated by: iGEM to SBOL conversion
description: Plac-rbs-luxI-Ter
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
mutable provenance: Plac, luxI, Ter
owned by: james
owned by: myers
mutable description: Plac-rbs-luxI-Ter. It produces luxI when there is enough IPTG or less lacI.
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: yue wu
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520029
component: BBa_K1520504
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_K1520504
sequence annotation: BBa_K1520029
sequence: https://synbiohub.org/public/igem/BBa_K1520009_sequence/1

BBa_K1520029
type: Component
persistent identity: component2384947
display id: component2384947
version: 1
top level: BBa_K1520009
definition: BBa_K1520029
access: public

BBa_K1520504
type: Component
persistent identity: component2384946
display id: component2384946
version: 1
top level: BBa_K1520009
definition: BBa_K1520504
access: public

BBa_R0010
type: Component
persistent identity: component2384937
display id: component2384937
version: 1
top level: BBa_K1520009
definition: LacI
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384937
display id: annotation2384937
version: 1
top level: BBa_K1520009
location: https://synbiohub.org/public/igem/BBa_K1520009/annotation2384937/range2384937/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1520009/annotation2384937/range2384937/1
type: Range
persistent identity: range2384937
display id: range2384937
version: 1
top level: BBa_K1520009
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2384946
display id: annotation2384946
version: 1
top level: BBa_K1520009
location: https://synbiohub.org/public/igem/BBa_K1520009/annotation2384946/range2384946/1
component: BBa_K1520504

https://synbiohub.org/public/igem/BBa_K1520009/annotation2384946/range2384946/1
type: Range
persistent identity: range2384946
display id: range2384946
version: 1
top level: BBa_K1520009
direction: direction/reverse
start: 209
end: 869
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384947
display id: annotation2384947
version: 1
top level: BBa_K1520009
location: https://synbiohub.org/public/igem/BBa_K1520009/annotation2384947/range2384947/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520009/annotation2384947/range2384947/1
type: Range
persistent identity: range2384947
display id: range2384947
version: 1
top level: BBa_K1520009
direction: direction/reverse
start: 878
end: 1006
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_K1520502
type: ComponentDefinition
persistent identity: BBa K1520502
display id: BBa_K1520502
version: 1
was generated by: iGEM to SBOL conversion
description: Rbs-lacI(scarless)
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
mutable provenance: It is an improvement of lacI by adding a rbs.
owned by: james
owned by: myers
mutable description: Rbs-lacI(scarless) It is an improvement of lacI by adding a rbs.
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: yue wu
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: rbs
sequence annotation: lacI
sequence: https://synbiohub.org/public/igem/BBa_K1520502_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2384863
display id: annotation2384863
version: 1
top level: BBa_K1520502
location: https://synbiohub.org/public/igem/BBa_K1520502/annotation2384863/range2384863/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1520502/annotation2384863/range2384863/1
type: Range
persistent identity: range2384863
display id: range2384863
version: 1
top level: BBa_K1520502
direction: direction/forward
start: 1
end: 18
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2384864
display id: annotation2384864
version: 1
top level: BBa_K1520502
location: https://synbiohub.org/public/igem/BBa_K1520502/annotation2384864/range2384864/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1520502/annotation2384864/range2384864/1
type: Range
persistent identity: range2384864
display id: range2384864
version: 1
top level: BBa_K1520502
direction: direction/forward
start: 19
end: 1171
orientation: inline

BBa_K1520504
type: ComponentDefinition
persistent identity: BBa K1520504
display id: BBa_K1520504
version: 1
was generated by: iGEM to SBOL conversion
description: Rbs-luxI(scarless)
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
mutable provenance: It is an improvement of luxI(BBa_C0061) by adding a rbs.
owned by: james
owned by: myers
mutable description: Rbs-luxI(scarless) It is an improvement of luxI(BBa_C0061) by adding a rbs.
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: yue wu
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: rbs
sequence annotation: luxI
sequence: https://synbiohub.org/public/igem/BBa_K1520504_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2384869
display id: annotation2384869
version: 1
top level: BBa_K1520504
location: https://synbiohub.org/public/igem/BBa_K1520504/annotation2384869/range2384869/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1520504/annotation2384869/range2384869/1
type: Range
persistent identity: range2384869
display id: range2384869
version: 1
top level: BBa_K1520504
direction: direction/forward
start: 1
end: 18
orientation: inline

luxI
type: SequenceAnnotation
persistent identity: annotation2384870
display id: annotation2384870
version: 1
top level: BBa_K1520504
location: https://synbiohub.org/public/igem/BBa_K1520504/annotation2384870/range2384870/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1520504/annotation2384870/range2384870/1
type: Range
persistent identity: range2384870
display id: range2384870
version: 1
top level: BBa_K1520504
direction: direction/forward
start: 19
end: 661
orientation: inline

https://synbiohub.org/public/igem/BBa_K1520023_sequence/1
type: Sequence
persistent identity: BBa K1520023 sequence
display id: BBa_K1520023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaattaagcatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaattaagcatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtatta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1520012_sequence/1
type: Sequence
persistent identity: BBa K1520012 sequence
display id: BBa_K1520012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaattaagcatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagaaagaggagaaattaagcatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1520020_sequence/1
type: Sequence
persistent identity: BBa K1520020 sequence
display id: BBa_K1520020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaattaagcatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaattaagcatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtatta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0062_sequence/1
type: Sequence
persistent identity: BBa R0062 sequence
display id: BBa_R0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1520029_sequence/1
type: Sequence
persistent identity: BBa K1520029 sequence
display id: BBa_K1520029_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1520500_sequence/1
type: Sequence
persistent identity: BBa K1520500 sequence
display id: BBa_K1520500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaattaagcatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1520003_sequence/1
type: Sequence
persistent identity: BBa K1520003 sequence
display id: BBa_K1520003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaattaagcatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1520501_sequence/1
type: Sequence
persistent identity: BBa K1520501 sequence
display id: BBa_K1520501_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaattaagcatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1520019_sequence/1
type: Sequence
persistent identity: BBa K1520019 sequence
display id: BBa_K1520019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaattaagcatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaattaagcatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtatta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1520008_sequence/1
type: Sequence
persistent identity: BBa K1520008 sequence
display id: BBa_K1520008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaattaagcatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1520009_sequence/1
type: Sequence
persistent identity: BBa K1520009 sequence
display id: BBa_K1520009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaattaagcatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1520502_sequence/1
type: Sequence
persistent identity: BBa K1520502 sequence
display id: BBa_K1520502_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaattaagcatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1520504_sequence/1
type: Sequence
persistent identity: BBa K1520504 sequence
display id: BBa_K1520504_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaattaagcatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z