##gff-version 3
##sequence-region BBa_K1520025 1 1987
BBa_K1520025	.	engineered_region	1	931	.	+	0	ID=BBa_K1520014;Name=BBa_K1520014
BBa_K1520025	.	terminator	803	931	.	+	0	ID=BBa_K1520029;Name=BBa_K1520029;Parent=BBa_K1520014
BBa_K1520025	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K1520014
BBa_K1520025	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1520025	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1520025	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1520025	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1520025	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1520025	.	engineered_region	63	794	.	+	0	ID=BBa_K1520500;Name=BBa_K1520500;Parent=BBa_K1520014
BBa_K1520025	.	engineered_region	940	1987	.	+	0	ID=BBa_K1520015;Name=BBa_K1520015
BBa_K1520025	.	promoter	940	1139	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K1520015
BBa_K1520025	.	CDS	940	1027	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1520025	.	promoter	1100	1105	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1520025	.	non_covalent_binding_site	1105	1139	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1520025	.	engineered_region	940	1139	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1520025	.	promoter	1076	1081	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1520025	.	start_codon	1112	1112	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1520025	.	non_covalent_binding_site	1028	1065	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1520025	.	terminator	1859	1987	.	+	0	ID=BBa_K1520029;Name=BBa_K1520029;Parent=BBa_K1520015
BBa_K1520025	.	engineered_region	1148	1850	.	+	0	ID=BBa_K1520505;Name=BBa_K1520505;Parent=BBa_K1520015
BBa_K1520025	.	CDS	1166	1850	.	+	0	ID=annotation2384878;Name=tetR;Parent=BBa_K1520505
BBa_K1520025	.	ribosome_entry_site	1148	1165	.	+	0	ID=annotation2384877;Name=rbs;Parent=BBa_K1520505
>BBa_K1520025
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaattaag
catggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctcc
gtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaagg
tgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaa
gcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgag
gacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggca
ccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccga
ggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagacc
acctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacg
aggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagta
ataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgca
aaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtga
gcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttg
tgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaattaagcatgtccagattagataaaag
taaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaag
ctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgt
tagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttt
tagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaact
ctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggc
attttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatag
tatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaa
ttgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtag
cttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagac
tgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctt
tctgcgtttata