LOCUS       BBa_K1520026            3346 bp    DNA     linear       18-May-2024
DEFINITION  Ptet-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter
ACCESSION   BBa_K1520026
VERSION     BBa_K1520026.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    63..794
                     /label=BBa_K1520500
     terminator      803..931
                     /label=BBa_K1520029
     misc_feature    1..931
                     /label=BBa_K1520014
     CDS             940..1027
                     /label=end of LacI coding region (inactive)
     misc_feature    940..1139
                     /label=BBa_R0010
     misc_feature    1028..1065
                     /label=CAP binding site
     promoter        1076..1081
                     /label=-35
     promoter        1100..1105
                     /label=-10
     misc_feature    1105..1139
                     /label=LacI binding site
     misc_feature    1112..1112
                     /label=start
     promoter        940..1139
                     /label=BBa_R0010
     RBS             1148..1165
                     /label=rbs
     CDS             1166..1850
                     /label=tetR
     misc_feature    1148..1850
                     /label=BBa_K1520505
     terminator      1859..1987
                     /label=BBa_K1520029
     misc_feature    940..1987
                     /label=BBa_K1520015
     misc_feature    1..1987
                     /label=BBa_K1520025
     promoter        1996..2030
                     /label=BBa_J23100
     RBS             2039..2056
                     /label=rbs
     CDS             2057..3209
                     /label=lacI
     misc_feature    2039..3209
                     /label=BBa_K1520502
     terminator      3218..3346
                     /label=BBa_K1520029
     misc_feature    1996..3346
                     /label=BBa_K1520008
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaattaagc atggtgagca agggcgagga ggataacatg gccatcatca
      121 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga
      181 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
      241 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct
      301 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg
      361 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc
      421 aggactcctc cttgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact
      481 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc
      541 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg
      601 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc
      661 tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca
      721 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc
      781 tgtacaagta ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
      841 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
      901 tcaccttcgg gtgggccttt ctgcgtttat atactagagc aatacgcaaa ccgcctctcc
      961 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     1021 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     1081 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacat
     1141 actagagaaa gaggagaaat taagcatgtc cagattagat aaaagtaaag tgattaacag
     1201 cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca
     1261 gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct
     1321 cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg
     1381 ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag
     1441 tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac
     1501 tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata
     1561 tgcactcagc gctgtggggc attttacttt aggttgcgta ttggaagatc aagagcatca
     1621 agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca
     1681 agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt
     1741 gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtccgctg caaacgacga
     1801 aaactacgct ttagtagctt aataacactg atagtgctag tgtagatcac tactagagcc
     1861 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1921 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1981 gtttatatac tagagttgac ggctagctca gtcctaggta cagtgctagc tactagagaa
     2041 agaggagaaa ttaagcatgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta
     2101 tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc
     2161 gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt
     2221 ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc
     2281 cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc
     2341 cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca
     2401 caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga
     2461 tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga
     2521 ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga
     2581 gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt
     2641 ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc
     2701 gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat
     2761 gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg
     2821 cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg
     2881 atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga
     2941 ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc
     3001 ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc
     3061 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     3121 ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa aactacgctt tagtagctta
     3181 ataactctga tagtgctagt gtagatctct actagagcca ggcatcaaat aaaacgaaag
     3241 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     3301 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//