LOCUS BBa_K152005 5412 bp DNA linear 18-May-2024 DEFINITION Promoterless Synthetic Operon of CrtE,B,I,Y and GFP ACCESSION BBa_K152005 VERSION BBa_K152005.1 FEATURES Location/Qualifiers RBS 3..10 /label=rbs CDS 18..926 /label=cds misc_feature 1..928 /label=BBa_K152001 RBS 939..946 /label=rbs CDS 954..1844 /label=cds misc_feature 937..1846 /label=BBa_K152002 misc_feature 1855..1857 /label=SacI RBS 1858..1864 /label=rbs RBS 1855..1864 /label=BBa_J15001 misc_feature 2348..2353 /label=PstI site removed misc_feature 2759..2764 /label=PstI site removed CDS 1871..3349 /label=crtI coding sequence CDS 1871..3352 /label=BBa_K118003 RBS 3364..3370 /label=rbs CDS 3378..4526 /label=cds misc_feature 3361..4528 /label=BBa_K152003 RBS 4537..4549 /label=RBS-3\Weak RBS 4543..4546 /label=RBS misc_feature 4537..4549 /label=BBa_B0032 RBS 4537..4549 /label=BBa_B0032 CDS 4556..5275 /label=GFP protein CDS 4556..5275 /label=BBa_E0040 stem_loop 5295..5338 /label=stem_loop misc_feature 5284..5363 /label=BBa_B0010 terminator 5284..5363 /label=BBa_B0010 stem_loop 5379..5398 /label=T7 TE misc_feature 5405..5405 /label=stop polyA_site 5399..5412 /label=polya misc_feature 5372..5412 /label=BBa_B0012 terminator 5372..5412 /label=BBa_B0012 ORIGIN 1 agaaggagga ttaccatatg acggtctgcg caaaaaaaca cgttcatctc actcgcgatg 61 ctgcggagca gttactggct gatattgatc gacgccttga tcagttattg cccgtggagg 121 gagaacggga tgttgtgggt gccgcgatgc gtgaaggtgc gctggcaccg ggaaaacgta 181 ttcgccccat gttgctgttg ctgaccgccc gcgatctggg ttgcgctgtc agccatgacg 241 gattactgga tttggcctgt gcggtggaaa tggtccacgc ggcttcgctg atccttgacg 301 atatgccctg catggacgat gcgaagctgc ggcgcggacg ccctaccatt cattctcatt 361 acggagagca tgtggcaata ctggcggcgg ttgccttgct gagtaaagcc tttggcgtaa 421 ttgccgatgc agatggcctc acgccgctgg caaaaaatcg ggcggtttct gaactgtcaa 481 acgccatcgg catgcaagga ttggttcagg gtcagttcaa ggatctgtct gaaggggata 541 agccgcgcag cgctgaagct attttgatga cgaatcactt taaaaccagc acgctgttct 601 gtgcctccat gcagatggcc tcgattgttg cgaatgcctc cagcgaagcg cgtgattgcc 661 tgcatcgttt ttcacttgat cttggtcagg catttcaact gctggacgat ttgaccgatg 721 gcatgaccga caccggtaag gatagcaatc aggacgccgg taaatcgacg ctggtcaatc 781 tgttaggccc gagggcggtt gaagaacgtc tgagacaaca tcttcagctt gccagtgagc 841 atctctctgc ggcctgccaa cacgggcacg ccacccaaca ttttattcag gcgtggtttg 901 acaaaaaact cgctgccgtc agttaactta ctagagagaa ggaggattac aaaatggcag 961 ttggctcgaa aagttttgcg acagcctcaa agttatttga tgcaaaaacc cggcgcagcg 1021 tactgatgct ctacgcctgg tgccgccatt gtgacgatgt tattgacgat cagacgctgg 1081 gctttcaggc ccggcagcct gccttacaaa cgcccgaaca acgtctgatg caacttgaga 1141 tgaaaacgcg ccaggcctat gcaggatcgc agatgcacga accggcgttt gcggcttttc 1201 aggaagtggc tatggctcat gatatcgccc cggcttacgc gtttgatcat ctggaaggct 1261 tcgccatgga tgtacgcgaa gcgcaataca gccaactgga tgatacgctg cgctattgct 1321 atcacgttgc aggcgttgtc ggcttgatga tggcgcaaat catgggcgta cgggataacg 1381 ccacgctgga ccgcgcctgt gaccttgggc tggcatttca gttgaccaat attgctcgcg 1441 atattgtgga cgatgcgcat gcgggccgct gttatctgcc ggcaagctgg ctggagcatg 1501 aaggtctgaa caaagagaat tatgcggcac ctgaaaaccg tcaggcgctg agccgtatcg 1561 cccgtcgttt ggtgcaggaa gcagaacctt actatttgtc tgccacagcc ggcctggcag 1621 ggttgcccct gcgttccgcc tgggcaatcg ctacggcgaa gcaggtttac cggaaaatag 1681 gtgtcaaagt tgaacaggcc ggtcagcaag cctgggatca gcggcagtca acgaccacgc 1741 ccgaaaaatt aacgctgctg ctggccgcct ctggtcaggc ccttacttcc cggatgcggg 1801 ctcatcctcc ccgccctgcg catctctggc agcgcccgct ctaacttact agagctcaag 1861 gaggtactag atgaaaccaa ctacggtaat tggtgcaggc ttcggtggcc tggcactggc 1921 aattcgtcta caagctgcgg ggatccccgt cttactgctt gaacaacgtg ataaacccgg 1981 cggtcgggct tatgtctacg aggatcaggg gtttaccttt gatgcaggcc cgacggttat 2041 caccgatccc agtgccattg aagaactgtt tgcactggca ggaaaacagt taaaagagta 2101 tgtcgaactg ctgccggtta cgccgtttta ccgcctgtgt tgggagtcag ggaaggtctt 2161 taattacgat aacgatcaaa cccggctcga agcgcagatt cagcagttta atccccgcga 2221 tgtcgaaggt tatcgtcagt ttctggacta ttcacgcgcg gtgtttaaag aaggctatct 2281 aaagctcggt actgtccctt ttttatcgtt cagagacatg cttcgcgccg cacctcaact 2341 ggcgaaactg caagcatgga gaagcgttta cagtaaggtt gccagttaca tcgaagatga 2401 acatctgcgc caggcgtttt ctttccactc gctgttggtg ggcggcaatc ccttcgccac 2461 ctcatccatt tatacgttga tacacgcgct ggagcgtgag tggggcgtct ggtttccgcg 2521 tggcggcacc ggcgcattag ttcaggggat gataaagctg tttcaggatc tgggtggcga 2581 agtcgtgtta aacgccagag tcagccatat ggaaacgaca ggaaacaaga ttgaagccgt 2641 gcatttagag gacggtcgca ggttcctgac gcaagccgtc gcgtcaaatg cagatgtggt 2701 tcatacctat cgcgacctgt taagccagca ccctgccgcg gttaagcagt ccaacaaact 2761 gcaaactaag cgcatgagta actctctgtt tgtgctctat tttggtttga atcaccatca 2821 tgatcagctc gcgcatcaca cggtttgttt cggcccgcgt taccgcgagc tgattgacga 2881 aatttttaat catgatggcc tcgcagagga cttctcactt tatctgcacg cgccctgtgt 2941 cacggattcg tcactggcgc ctgaaggttg cggcagttac tatgtgttgg cgccggtgcc 3001 gcatttaggc accgcgaacc tcgactggac ggttgagggg ccaaaactac gcgaccgtat 3061 ttttgcgtac cttgagcagc attacatgcc tggcttacgg agtcagctgg tcacgcaccg 3121 gatgtttacg ccgtttgatt ttcgcgacca gcttaatgcc tatcatggct cagccttttc 3181 tgtggagccc gttcttaccc agagcgcctg gtttcggccg cataaccgcg ataaaaccat 3241 tactaatctc tacctggtcg gcgcaggcac gcatcccggc gcaggcattc ctggcgtcat 3301 cggctcggca aaagcgacag caggtttgat gctggaggat ctgatataat aatactagag 3361 agaaggagga ttacaaaatg caaccgcatt atgatctgat tctcgtgggg gctggactcg 3421 cgaatggcct tatcgccctg cgtcttcagc agcagcaacc tgatatgcgt attttgctta 3481 tcgacgccgc accccaggcg ggcgggaatc atacgtggtc atttcaccac gatgatttga 3541 ctgagagcca acatcgttgg atagctccgc tggtggttca tcactggccc gactatcagg 3601 tacgctttcc cacacgccgt cgtaagctga acagcggcta cttttgtatt acttctcagc 3661 gtttcgctga ggttttacag cgacagtttg gcccgcactt gtggatggat accgcggtcg 3721 cagaggttaa tgcggaatct gttcggttga aaaagggtca ggttatcggt gcccgcgcgg 3781 tgattgacgg gcggggttat gcggcaaatt cagcactgag cgtgggcttc caggcgttta 3841 ttggccagga atggcgattg agccacccgc atggtttatc gtctcccatt atcatggatg 3901 ccacggtcga tcagcaaaat ggttatcgct tcgtgtacag cctgccgctc tcgccgacca 3961 gattgttaat tgaagacacg cactatattg ataatgcgac attagatcct gaatgcgcgc 4021 ggcaaaatat ttgcgactat gccgcgcaac agggttggca gcttcagaca ctgctgcgag 4081 aagaacaggg cgccttaccc attactctgt cgggcaatgc cgacgcattc tggcagcagc 4141 gccccctggc ctgtagtgga ttacgtgccg gtctgttcca tcctaccacc ggctattcac 4201 tgccgctggc ggttgccgtg gccgaccgcc tgagtgcact tgatgtcttt acgtcggcct 4261 caattcacca tgccattacg cattttgccc gcgagcgctg gcagcagcag ggctttttcc 4321 gcatgctgaa tcgcatgctg tttttagccg gacccgccga ttcacgctgg cgggttatgc 4381 agcgttttta tggtttacct gaagatttaa ttgcccgttt ttatgcggga aaactcacgc 4441 tgaccgatcg gctacgtatt ctgagcggca agccgcctgt tccggtatta gcagcattgc 4501 aagccattat gacgactcat cgttaactta ctagagtcac acaggaaagt actagatgcg 4561 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt 4621 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact 4681 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac 4741 tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga 4801 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga 4861 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag 4921 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga 4981 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa 5041 agttaacttc aaaattagac acaacattga agatggaagc gttcaactag cagaccatta 5101 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc 5161 cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga 5221 gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat aataatacta 5281 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 5341 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 5401 tctgcgttta ta //