##gff-version 3
##sequence-region BBa_K152005 1 5412
BBa_K152005	.	CDS	4556	5275	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K152005	.	CDS	4556	5275	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K152005	.	terminator	5284	5363	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K152005	.	engineered_region	5284	5363	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K152005	.	stem_loop	5295	5338	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K152005	.	ribosome_entry_site	4537	4549	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K152005	.	ribosome_entry_site	4537	4549	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K152005	.	engineered_region	4537	4549	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K152005	.	ribosome_entry_site	4543	4546	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K152005	.	ribosome_entry_site	1855	1864	.	+	0	ID=BBa_J15001;Name=BBa_J15001
BBa_K152005	.	ribosome_entry_site	1858	1864	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
BBa_K152005	.	sequence_feature	1855	1857	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_K152005	.	CDS	1871	3352	.	+	0	ID=BBa_K118003;Name=BBa_K118003
BBa_K152005	.	point_mutation	2348	2353	.	+	0	ID=annotation1968244;Name=PstI site removed;Parent=BBa_K118003
BBa_K152005	.	CDS	1871	3349	.	+	0	ID=annotation1995702;Name=crtI coding sequence;Parent=BBa_K118003
BBa_K152005	.	point_mutation	2759	2764	.	+	0	ID=annotation1968245;Name=PstI site removed;Parent=BBa_K118003
BBa_K152005	.	engineered_region	937	1846	.	+	0	ID=BBa_K152002;Name=BBa_K152002
BBa_K152005	.	ribosome_entry_site	939	946	.	+	0	ID=annotation1999565;Name=rbs;Parent=BBa_K152002
BBa_K152005	.	CDS	954	1844	.	+	0	ID=annotation1999566;Name=cds;Parent=BBa_K152002
BBa_K152005	.	terminator	5372	5412	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K152005	.	stop_codon	5405	5405	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K152005	.	polyA_site	5399	5412	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K152005	.	stem_loop	5379	5398	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K152005	.	engineered_region	5372	5412	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K152005	.	engineered_region	3361	4528	.	+	0	ID=BBa_K152003;Name=BBa_K152003
BBa_K152005	.	CDS	3378	4526	.	+	0	ID=annotation1999564;Name=cds;Parent=BBa_K152003
BBa_K152005	.	ribosome_entry_site	3364	3370	.	+	0	ID=annotation1999563;Name=rbs;Parent=BBa_K152003
BBa_K152005	.	engineered_region	1	928	.	+	0	ID=BBa_K152001;Name=BBa_K152001
BBa_K152005	.	ribosome_entry_site	3	10	.	+	0	ID=annotation1999567;Name=rbs;Parent=BBa_K152001
BBa_K152005	.	CDS	18	926	.	+	0	ID=annotation1999568;Name=cds;Parent=BBa_K152001
>BBa_K152005
agaaggaggattaccatatgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggc
tgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggt
gcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatg
acggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatgga
cgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgcc
ttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaac
tgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgc
tgaagctattttgatgacgaatcactttaaaaccagcacgctgttctgtgcctccatgcagatggcctcgattgttgcg
aatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatt
tgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggccc
gagggcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcac
gccacccaacattttattcaggcgtggtttgacaaaaaactcgctgccgtcagttaacttactagagagaaggaggatt
acaaaatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgat
gctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgcctta
caaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccgg
cgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgc
catggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggc
ttgatgatggcgcaaatcatgggcgtacgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagt
tgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatga
aggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaa
gcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcga
agcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccac
gcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccct
gcgcatctctggcagcgcccgctctaacttactagagctcaaggaggtactagatgaaaccaactacggtaattggtgc
aggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaacgtgataaa
cccggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcaggcccgacggttatcaccgatcccagtg
ccattgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgctgccggttacgccgttttaccg
cctgtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggctcgaagcgcagattcagcagtttaat
ccccgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtttaaagaaggctatctaaagctcggta
ctgtcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcgaaactgcaagcatggagaagcgttta
cagtaaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctttccactcgctgttggtgggcggcaat
cccttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtggggcgtctggtttccgcgtggcggca
ccggcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaagtcgtgttaaacgccagagtcagcca
tatggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgcaggttcctgacgcaagccgtcgcgtca
aatgcagatgtggttcatacctatcgcgacctgttaagccagcaccctgccgcggttaagcagtccaacaaactgcaaa
ctaagcgcatgagtaactctctgtttgtgctctattttggtttgaatcaccatcatgatcagctcgcgcatcacacggt
ttgtttcggcccgcgttaccgcgagctgattgacgaaatttttaatcatgatggcctcgcagaggacttctcactttat
ctgcacgcgccctgtgtcacggattcgtcactggcgcctgaaggttgcggcagttactatgtgttggcgccggtgccgc
atttaggcaccgcgaacctcgactggacggttgaggggccaaaactacgcgaccgtatttttgcgtaccttgagcagca
ttacatgcctggcttacggagtcagctggtcacgcaccggatgtttacgccgtttgattttcgcgaccagcttaatgcc
tatcatggctcagccttttctgtggagcccgttcttacccagagcgcctggtttcggccgcataaccgcgataaaacca
ttactaatctctacctggtcggcgcaggcacgcatcccggcgcaggcattcctggcgtcatcggctcggcaaaagcgac
agcaggtttgatgctggaggatctgatataataatactagagagaaggaggattacaaaatgcaaccgcattatgatct
gattctcgtgggggctggactcgcgaatggccttatcgccctgcgtcttcagcagcagcaacctgatatgcgtattttg
cttatcgacgccgcaccccaggcgggcgggaatcatacgtggtcatttcaccacgatgatttgactgagagccaacatc
gttggatagctccgctggtggttcatcactggcccgactatcaggtacgctttcccacacgccgtcgtaagctgaacag
cggctacttttgtattacttctcagcgtttcgctgaggttttacagcgacagtttggcccgcacttgtggatggatacc
gcggtcgcagaggttaatgcggaatctgttcggttgaaaaagggtcaggttatcggtgcccgcgcggtgattgacgggc
ggggttatgcggcaaattcagcactgagcgtgggcttccaggcgtttattggccaggaatggcgattgagccacccgca
tggtttatcgtctcccattatcatggatgccacggtcgatcagcaaaatggttatcgcttcgtgtacagcctgccgctc
tcgccgaccagattgttaattgaagacacgcactatattgataatgcgacattagatcctgaatgcgcgcggcaaaata
tttgcgactatgccgcgcaacagggttggcagcttcagacactgctgcgagaagaacagggcgccttacccattactct
gtcgggcaatgccgacgcattctggcagcagcgccccctggcctgtagtggattacgtgccggtctgttccatcctacc
accggctattcactgccgctggcggttgccgtggccgaccgcctgagtgcacttgatgtctttacgtcggcctcaattc
accatgccattacgcattttgcccgcgagcgctggcagcagcagggctttttccgcatgctgaatcgcatgctgttttt
agccggacccgccgattcacgctggcgggttatgcagcgtttttatggtttacctgaagatttaattgcccgtttttat
gcgggaaaactcacgctgaccgatcggctacgtattctgagcggcaagccgcctgttccggtattagcagcattgcaag
ccattatgacgactcatcgttaacttactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcact
ggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtg
atgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcac
tactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatg
cccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttg
aaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaatt
ggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaatt
agacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcc
ttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtcct
tcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttata