BBa_K152006 1 BBa_K152006 frdBCD operon from E. coli (missing the first gene fumarate oxidase) 2008-10-30T12:00:00Z 2015-05-08T01:10:49Z E. coli DH5 alpha genomic DNA. This partial operon contains the three operon reading frames found after the large fumarate oxidase genes. Fumarate reductase catalyzes the reduction of fumarate to succinate in the terminal step of anaerobic electron transfer to fumarate and is a membrane-bound complex composed of four different polypeptides designated FrdA, FrdB, FrdC, and FrdD. FrdA is a large protein that protein binds flavin adenine dinucleotide. FrdB contains the reactive iron-sulfur cofactors, while FrdC and FrdD are membrane proteins which bind FrdA and B to the inner side of the cytoplasmic membrane. J Bacteriol. 1987 July; 169(7): 3340???3349. Overexpression of FrdABCD results in a doubling of E. coli cytoplasmid membrane (J Bacteriol. 1984 May; 158(2): 590-596), so it is hoped overexpression of FrdBCD will also increase membrane area. false false _233_ 0 2886 9 It's complicated true FrdA was not included in the production of this biobrick, on account of it containing several Biobrick sites; too many to remove through site directed mutagenesis. Hopefully FrdBCD is enough to induce the membrane doubling phenotype. true David Johnston Monje annotation1999571 1 rbs range1999571 1 12 20 BBa_K152006_sequence 1 agacaataagaaggagaaggcgaatggctgagatgaaaaacctgaaaattgaggtggtgcgctataacccgaaagtcgataccgcaccgcatagcgcattctatgaagtgccttatgacgcaactacctcattactggatgcgctgggctacatcaaagacaacctggcaccggacctgagctaccgctggtcctgccgtatggcgatttgtggttcctgcggcatgatggttaacaacgtgccaaaactggcatgtaaaaccttcctgcgtgattacaccgacggtatgaaggttgaagcgttagctaacttcccgattgaacgcgatctggtggtcgatatgacccacttcatcgaaagtctggaagcgatcaaaccgtacatcatcggcaactcccgcaccgcggatcagggtactaacatccagaccccggcgcagatggcgaagtatcaccagttctccggttgcatcaactgtggtttgtgctacgccgcgtgcccgcagtttggcctgaacccagagttcatcggtccggctgccattacgctggcgcatcgttataacgaagatagccgcgaccacggtaagaaggagcgtatggcgcagttgaacagccagaacggcgtatggagctgtactttcgtgggctactgctccgaagtctgcccgaaacacgtcgatccggctgcggccattcagcagggcaaagtagaaagttcgaaagactttcttatcgcgaccctgaaaccacgctaaggagtgcaacatgacgactaaacgtaaaccgtatgtacggccaatgacgtccacctggtggaaaaaattgccgttttatcgcttttacatgctgcgcgaaggcacggcggttccggctgtgtggttcagcattgaactgattttcgggctgtttgccctgaaaaatggcccggaagcctgggcgggattcgtcgactttttacaaaacccggttatcgtgatcattaacctgatcactctggcggcagctctgctgcacaccaaaacctggtttgaactggcaccgaaagcggccaatatcattgtaaaagacgaaaaaatgggaccagagccaattatcaaaagtctctgggcggtaactgtggttgccaccatcgtaatcctgtttgttgccctgtactggtaaggagcctgagatgattaatccaaatccaaagcgttctgacgaaccggtattctggggcctcttcggggccggtggtatgtggagcgccatcattgcgccggtgatgatcctgctggtgggtattctgctgccactggggttgtttccgggtgatgcgctgagctacgagcgcgttctggcgttcgcgcagagcttcattggtcgcgtattcctgttcctgatgatcgttctgccgctgtggtgtggtttacaccgtatgcaccacgcgatgcacgatctgaaaatccacgtacctgcgggcaaatgggttttctacggtctggctgctatcctgacagttgtcacgctgattggtgtcgttacaatctaacgcatcgcact igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z