LOCUS BBa_K152007 6965 bp DNA linear 29-Nov-2024 DEFINITION Synthetic operon of CrtEBIY and FrdBCD and GFP ACCESSION BBa_K152007 VERSION BBa_K152007.1 FEATURES Location/Qualifiers RBS 3..10 /label=rbs CDS 18..926 /label=cds misc_feature 1..928 /label=BBa_K152001 RBS 939..946 /label=rbs CDS 954..1844 /label=cds misc_feature 937..1846 /label=BBa_K152002 misc_feature 1855..1857 /label=SacI RBS 1858..1864 /label=rbs RBS 1855..1864 /label=BBa_J15001 misc_feature 2348..2353 /label=PstI site removed misc_feature 2759..2764 /label=PstI site removed CDS 1871..3349 /label=crtI coding sequence CDS 1871..3352 /label=BBa_K118003 misc_feature 1855..3352 /label=BBa_K118005 RBS 3372..3380 /label=rbs misc_feature 3361..4905 /label=BBa_K152006 RBS 4917..4923 /label=rbs CDS 4931..6079 /label=cds misc_feature 4914..6081 /label=BBa_K152003 RBS 6090..6102 /label=RBS-3\Weak RBS 6096..6099 /label=RBS misc_feature 6090..6102 /label=BBa_B0032 RBS 6090..6102 /label=BBa_B0032 CDS 6109..6828 /label=GFP protein CDS 6109..6828 /label=BBa_E0040 stem_loop 6848..6891 /label=stem_loop misc_feature 6837..6916 /label=BBa_B0010 terminator 6837..6916 /label=BBa_B0010 stem_loop 6932..6951 /label=T7 TE misc_feature 6958..6958 /label=stop polyA_site 6952..6965 /label=polya misc_feature 6925..6965 /label=BBa_B0012 terminator 6925..6965 /label=BBa_B0012 misc_feature 6090..6965 /label=BBa_E0240 ORIGIN 1 agaaggagga ttaccatatg acggtctgcg caaaaaaaca cgttcatctc actcgcgatg 61 ctgcggagca gttactggct gatattgatc gacgccttga tcagttattg cccgtggagg 121 gagaacggga tgttgtgggt gccgcgatgc gtgaaggtgc gctggcaccg ggaaaacgta 181 ttcgccccat gttgctgttg ctgaccgccc gcgatctggg ttgcgctgtc agccatgacg 241 gattactgga tttggcctgt gcggtggaaa tggtccacgc ggcttcgctg atccttgacg 301 atatgccctg catggacgat gcgaagctgc ggcgcggacg ccctaccatt cattctcatt 361 acggagagca tgtggcaata ctggcggcgg ttgccttgct gagtaaagcc tttggcgtaa 421 ttgccgatgc agatggcctc acgccgctgg caaaaaatcg ggcggtttct gaactgtcaa 481 acgccatcgg catgcaagga ttggttcagg gtcagttcaa ggatctgtct gaaggggata 541 agccgcgcag cgctgaagct attttgatga cgaatcactt taaaaccagc acgctgttct 601 gtgcctccat gcagatggcc tcgattgttg cgaatgcctc cagcgaagcg cgtgattgcc 661 tgcatcgttt ttcacttgat cttggtcagg catttcaact gctggacgat ttgaccgatg 721 gcatgaccga caccggtaag gatagcaatc aggacgccgg taaatcgacg ctggtcaatc 781 tgttaggccc gagggcggtt gaagaacgtc tgagacaaca tcttcagctt gccagtgagc 841 atctctctgc ggcctgccaa cacgggcacg ccacccaaca ttttattcag gcgtggtttg 901 acaaaaaact cgctgccgtc agttaactta ctagagagaa ggaggattac aaaatggcag 961 ttggctcgaa aagttttgcg acagcctcaa agttatttga tgcaaaaacc cggcgcagcg 1021 tactgatgct ctacgcctgg tgccgccatt gtgacgatgt tattgacgat cagacgctgg 1081 gctttcaggc ccggcagcct gccttacaaa cgcccgaaca acgtctgatg caacttgaga 1141 tgaaaacgcg ccaggcctat gcaggatcgc agatgcacga accggcgttt gcggcttttc 1201 aggaagtggc tatggctcat gatatcgccc cggcttacgc gtttgatcat ctggaaggct 1261 tcgccatgga tgtacgcgaa gcgcaataca gccaactgga tgatacgctg cgctattgct 1321 atcacgttgc aggcgttgtc ggcttgatga tggcgcaaat catgggcgta cgggataacg 1381 ccacgctgga ccgcgcctgt gaccttgggc tggcatttca gttgaccaat attgctcgcg 1441 atattgtgga cgatgcgcat gcgggccgct gttatctgcc ggcaagctgg ctggagcatg 1501 aaggtctgaa caaagagaat tatgcggcac ctgaaaaccg tcaggcgctg agccgtatcg 1561 cccgtcgttt ggtgcaggaa gcagaacctt actatttgtc tgccacagcc ggcctggcag 1621 ggttgcccct gcgttccgcc tgggcaatcg ctacggcgaa gcaggtttac cggaaaatag 1681 gtgtcaaagt tgaacaggcc ggtcagcaag cctgggatca gcggcagtca acgaccacgc 1741 ccgaaaaatt aacgctgctg ctggccgcct ctggtcaggc ccttacttcc cggatgcggg 1801 ctcatcctcc ccgccctgcg catctctggc agcgcccgct ctaacttact agagctcaag 1861 gaggtactag atgaaaccaa ctacggtaat tggtgcaggc ttcggtggcc tggcactggc 1921 aattcgtcta caagctgcgg ggatccccgt cttactgctt gaacaacgtg ataaacccgg 1981 cggtcgggct tatgtctacg aggatcaggg gtttaccttt gatgcaggcc cgacggttat 2041 caccgatccc agtgccattg aagaactgtt tgcactggca ggaaaacagt taaaagagta 2101 tgtcgaactg ctgccggtta cgccgtttta ccgcctgtgt tgggagtcag ggaaggtctt 2161 taattacgat aacgatcaaa cccggctcga agcgcagatt cagcagttta atccccgcga 2221 tgtcgaaggt tatcgtcagt ttctggacta ttcacgcgcg gtgtttaaag aaggctatct 2281 aaagctcggt actgtccctt ttttatcgtt cagagacatg cttcgcgccg cacctcaact 2341 ggcgaaactg caagcatgga gaagcgttta cagtaaggtt gccagttaca tcgaagatga 2401 acatctgcgc caggcgtttt ctttccactc gctgttggtg ggcggcaatc ccttcgccac 2461 ctcatccatt tatacgttga tacacgcgct ggagcgtgag tggggcgtct ggtttccgcg 2521 tggcggcacc ggcgcattag ttcaggggat gataaagctg tttcaggatc tgggtggcga 2581 agtcgtgtta aacgccagag tcagccatat ggaaacgaca ggaaacaaga ttgaagccgt 2641 gcatttagag gacggtcgca ggttcctgac gcaagccgtc gcgtcaaatg cagatgtggt 2701 tcatacctat cgcgacctgt taagccagca ccctgccgcg gttaagcagt ccaacaaact 2761 gcaaactaag cgcatgagta actctctgtt tgtgctctat tttggtttga atcaccatca 2821 tgatcagctc gcgcatcaca cggtttgttt cggcccgcgt taccgcgagc tgattgacga 2881 aatttttaat catgatggcc tcgcagagga cttctcactt tatctgcacg cgccctgtgt 2941 cacggattcg tcactggcgc ctgaaggttg cggcagttac tatgtgttgg cgccggtgcc 3001 gcatttaggc accgcgaacc tcgactggac ggttgagggg ccaaaactac gcgaccgtat 3061 ttttgcgtac cttgagcagc attacatgcc tggcttacgg agtcagctgg tcacgcaccg 3121 gatgtttacg ccgtttgatt ttcgcgacca gcttaatgcc tatcatggct cagccttttc 3181 tgtggagccc gttcttaccc agagcgcctg gtttcggccg cataaccgcg ataaaaccat 3241 tactaatctc tacctggtcg gcgcaggcac gcatcccggc gcaggcattc ctggcgtcat 3301 cggctcggca aaagcgacag caggtttgat gctggaggat ctgatataat aatactagag 3361 agacaataag aaggagaagg cgaatggctg agatgaaaaa cctgaaaatt gaggtggtgc 3421 gctataaccc gaaagtcgat accgcaccgc atagcgcatt ctatgaagtg ccttatgacg 3481 caactacctc attactggat gcgctgggct acatcaaaga caacctggca ccggacctga 3541 gctaccgctg gtcctgccgt atggcgattt gtggttcctg cggcatgatg gttaacaacg 3601 tgccaaaact ggcatgtaaa accttcctgc gtgattacac cgacggtatg aaggttgaag 3661 cgttagctaa cttcccgatt gaacgcgatc tggtggtcga tatgacccac ttcatcgaaa 3721 gtctggaagc gatcaaaccg tacatcatcg gcaactcccg caccgcggat cagggtacta 3781 acatccagac cccggcgcag atggcgaagt atcaccagtt ctccggttgc atcaactgtg 3841 gtttgtgcta cgccgcgtgc ccgcagtttg gcctgaaccc agagttcatc ggtccggctg 3901 ccattacgct ggcgcatcgt tataacgaag atagccgcga ccacggtaag aaggagcgta 3961 tggcgcagtt gaacagccag aacggcgtat ggagctgtac tttcgtgggc tactgctccg 4021 aagtctgccc gaaacacgtc gatccggctg cggccattca gcagggcaaa gtagaaagtt 4081 cgaaagactt tcttatcgcg accctgaaac cacgctaagg agtgcaacat gacgactaaa 4141 cgtaaaccgt atgtacggcc aatgacgtcc acctggtgga aaaaattgcc gttttatcgc 4201 ttttacatgc tgcgcgaagg cacggcggtt ccggctgtgt ggttcagcat tgaactgatt 4261 ttcgggctgt ttgccctgaa aaatggcccg gaagcctggg cgggattcgt cgacttttta 4321 caaaacccgg ttatcgtgat cattaacctg atcactctgg cggcagctct gctgcacacc 4381 aaaacctggt ttgaactggc accgaaagcg gccaatatca ttgtaaaaga cgaaaaaatg 4441 ggaccagagc caattatcaa aagtctctgg gcggtaactg tggttgccac catcgtaatc 4501 ctgtttgttg ccctgtactg gtaaggagcc tgagatgatt aatccaaatc caaagcgttc 4561 tgacgaaccg gtattctggg gcctcttcgg ggccggtggt atgtggagcg ccatcattgc 4621 gccggtgatg atcctgctgg tgggtattct gctgccactg gggttgtttc cgggtgatgc 4681 gctgagctac gagcgcgttc tggcgttcgc gcagagcttc attggtcgcg tattcctgtt 4741 cctgatgatc gttctgccgc tgtggtgtgg tttacaccgt atgcaccacg cgatgcacga 4801 tctgaaaatc cacgtacctg cgggcaaatg ggttttctac ggtctggctg ctatcctgac 4861 agttgtcacg ctgattggtg tcgttacaat ctaacgcatc gcacttacta gagagaagga 4921 ggattacaaa atgcaaccgc attatgatct gattctcgtg ggggctggac tcgcgaatgg 4981 ccttatcgcc ctgcgtcttc agcagcagca acctgatatg cgtattttgc ttatcgacgc 5041 cgcaccccag gcgggcggga atcatacgtg gtcatttcac cacgatgatt tgactgagag 5101 ccaacatcgt tggatagctc cgctggtggt tcatcactgg cccgactatc aggtacgctt 5161 tcccacacgc cgtcgtaagc tgaacagcgg ctacttttgt attacttctc agcgtttcgc 5221 tgaggtttta cagcgacagt ttggcccgca cttgtggatg gataccgcgg tcgcagaggt 5281 taatgcggaa tctgttcggt tgaaaaaggg tcaggttatc ggtgcccgcg cggtgattga 5341 cgggcggggt tatgcggcaa attcagcact gagcgtgggc ttccaggcgt ttattggcca 5401 ggaatggcga ttgagccacc cgcatggttt atcgtctccc attatcatgg atgccacggt 5461 cgatcagcaa aatggttatc gcttcgtgta cagcctgccg ctctcgccga ccagattgtt 5521 aattgaagac acgcactata ttgataatgc gacattagat cctgaatgcg cgcggcaaaa 5581 tatttgcgac tatgccgcgc aacagggttg gcagcttcag acactgctgc gagaagaaca 5641 gggcgcctta cccattactc tgtcgggcaa tgccgacgca ttctggcagc agcgccccct 5701 ggcctgtagt ggattacgtg ccggtctgtt ccatcctacc accggctatt cactgccgct 5761 ggcggttgcc gtggccgacc gcctgagtgc acttgatgtc tttacgtcgg cctcaattca 5821 ccatgccatt acgcattttg cccgcgagcg ctggcagcag cagggctttt tccgcatgct 5881 gaatcgcatg ctgtttttag ccggacccgc cgattcacgc tggcgggtta tgcagcgttt 5941 ttatggttta cctgaagatt taattgcccg tttttatgcg ggaaaactca cgctgaccga 6001 tcggctacgt attctgagcg gcaagccgcc tgttccggta ttagcagcat tgcaagccat 6061 tatgacgact catcgttaac ttactagagt cacacaggaa agtactagat gcgtaaagga 6121 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg 6181 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt 6241 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc 6301 ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc 6361 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg 6421 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag 6481 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac 6541 tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac 6601 ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa 6661 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa 6721 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta 6781 acagctgctg ggattacaca tggcatggat gaactataca aataataata ctagagccag 6841 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 6901 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 6961 ttata //