LOCUS       BBa_K152011             1993 bp    DNA     linear       09-Jan-2025
DEFINITION  GFP RNAi construct
ACCESSION   BBa_K152011
VERSION     BBa_K152011.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             20..739
                     /label=GFP protein
     CDS             20..739
                     /label=BBa_E0040
     stem_loop       759..802
                     /label=stem_loop
     misc_feature    748..827
                     /label=BBa_B0010
     terminator      748..827
                     /label=BBa_B0010
     stem_loop       843..862
                     /label=T7 TE
     misc_feature    869..869
                     /label=stop
     polyA_site      863..876
                     /label=polya
     misc_feature    836..876
                     /label=BBa_B0012
     terminator      836..876
                     /label=BBa_B0012
     misc_feature    885..1109
                     /label=BBa_K152009
     RBS             1118..1130
                     /label=RBS-3\Weak
     RBS             1124..1127
                     /label=RBS
     misc_feature    1118..1130
                     /label=BBa_B0032
     RBS             1118..1130
                     /label=BBa_B0032
     CDS             1137..1856
                     /label=GFP protein
     CDS             1137..1856
                     /label=BBa_E0040
     stem_loop       1876..1919
                     /label=stem_loop
     misc_feature    1865..1944
                     /label=BBa_B0010
     terminator      1865..1944
                     /label=BBa_B0010
     stem_loop       1960..1979
                     /label=T7 TE
     misc_feature    1986..1986
                     /label=stop
     polyA_site      1980..1993
                     /label=polya
     misc_feature    1953..1993
                     /label=BBa_B0012
     terminator      1953..1993
                     /label=BBa_B0012
ORIGIN
        1 tcacacagga aagtactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat
       61 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      121 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      181 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
      241 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      301 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
      361 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
      421 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
      481 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
      541 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
      601 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
      661 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
      721 tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
      781 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
      841 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagttgatc atatgatgaa
      901 ttccattcta gacaactggt aagatggctg tcgcctcaga tatatatagt gatatgcact
      961 acaaagagga ggatagtcca tggcactaca acctaggtct ctttgtcaaa tatctctgtg
     1021 tgcaggtatt gtgatggact agtatgcggc cgcatgagga ggaaaaaaaa accccgcccc
     1081 tgacagggcg gggttttttt tctgcagaat actagagtca cacaggaaag tactagatgc
     1141 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg
     1201 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac
     1261 ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca acacttgtca
     1321 ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg
     1381 actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag
     1441 atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata
     1501 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg
     1561 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca
     1621 aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt
     1681 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt
     1741 ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg
     1801 agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa taataatact
     1861 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1921 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1981 ttctgcgttt ata
//