LOCUS BBa_K1520509 4955 bp DNA linear 18-May-2024 DEFINITION PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter- Pcons2-rbs-lacI-Ter ACCESSION BBa_K1520509 VERSION BBa_K1520509.1 FEATURES Location/Qualifiers promoter 1..200 /label=PgolTS CDS 212..553 /label=golS promoter 554..753 /label=PgolB promoter 1..753 /label=BBa_K1520506 RBS 762..779 /label=rbs CDS 780..1464 /label=tetR misc_feature 762..1464 /label=BBa_K1520505 terminator 1473..1601 /label=BBa_K1520029 misc_feature 1610..1628 /label=TetR 1 misc_feature 1610..1663 /label=BBa_R0040 promoter 1629..1634 /label=-35 misc_feature 1635..1653 /label=TetR 2 promoter 1652..1657 /label=-10 promoter 1610..1663 /label=BBa_R0040 misc_feature 1672..2403 /label=BBa_K1520500 terminator 2412..2540 /label=BBa_K1520029 misc_feature 1610..2540 /label=BBa_K1520014 CDS 2549..2636 /label=end of LacI coding region (inactive) misc_feature 2549..2748 /label=BBa_R0010 misc_feature 2637..2674 /label=CAP binding site promoter 2685..2690 /label=-35 promoter 2709..2714 /label=-10 misc_feature 2714..2748 /label=LacI binding site misc_feature 2721..2721 /label=start promoter 2549..2748 /label=BBa_R0010 RBS 2757..2774 /label=rbs CDS 2775..3459 /label=tetR misc_feature 2757..3459 /label=BBa_K1520505 terminator 3468..3596 /label=BBa_K1520029 misc_feature 2549..3596 /label=BBa_K1520015 misc_feature 1610..3596 /label=BBa_K1520025 promoter 3605..3639 /label=BBa_J23100 RBS 3648..3665 /label=rbs CDS 3666..4818 /label=lacI misc_feature 3648..4818 /label=BBa_K1520502 terminator 4827..4955 /label=BBa_K1520029 misc_feature 3605..4955 /label=BBa_K1520008 misc_feature 1610..4955 /label=BBa_K1520026 ORIGIN 1 acaacgaagg aaaggtcaag cgttcctgac gggtttttac ggggcgtggg tcggcatcgt 61 ggcgtaaatg tctcgcatca tcctctttta tgagccatct cacattctcg ccgaaccgtg 121 cagcctgaat acgcttgacc ttcccacaat ggcaagcttt aggctttctg ataccgaata 181 gtcaggatgg ggaagtcgtc ggaggagcgt catgaacatc ggtaaagcag ctaaagcatc 241 gaaagtctcg gccaaaatga ttcgctacta tgaacagatt ggtctgattc ccgcggcaag 301 tcggacggat tccggctatc gggcctatac ccaggctgat gttaatcaat tgcattttat 361 acgccgcgcg cgcgacctcg gtttttcagt tgctgaaatc agcgacttac tgaatctttg 421 gaataaccag tcgcggcaaa gcgctgacgt caaacgcctg gcgcagacgc acattgatga 481 actggacaga cgtatccaga acatgcagca catggcgcaa accctcaaag cgctgattca 541 ctgctgcgcc ggcgacgcgc tgccagattg ccccattctg catacgcttg gacaacctga 601 cgatagcgag ccggaggcgc gtaccggagc ggtattgcga cgtcctcgtc gccacggact 661 ggcaaagcgt ctgtaagtcc tgagattacg cttgaccttc caacactggc aaggtccaga 721 ctggcaacag ttcccacaca aaaggagttc acttactaga gaaagaggag aaattaagca 781 tgtccagatt agataaaagt aaagtgatta acagcgcatt agagctgctt aatgaggtcg 841 gaatcgaagg tttaacaacc cgtaaactcg cccagaagct aggtgtagag cagcctacat 901 tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt gagatgttag 961 ataggcacca tactcacttt tgccctttag aaggggaaag ctggcaagat tttttacgta 1021 ataacgctaa aagttttaga tgtgctttac taagtcatcg cgatggagca aaagtacatt 1081 taggtacacg gcctacagaa aaacagtatg aaactctcga aaatcaatta gcctttttat 1141 gccaacaagg tttttcacta gagaatgcat tatatgcact cagcgctgtg gggcatttta 1201 ctttaggttg cgtattggaa gatcaagagc atcaagtcgc taaagaagaa agggaaacac 1261 ctactactga tagtatgccg ccattattac gacaagctat cgaattattt gatcaccaag 1321 gtgcagagcc agccttctta ttcggccttg aattgatcat atgcggatta gaaaaacaac 1381 ttaaatgtga aagtgggtcc gctgcaaacg acgaaaacta cgctttagta gcttaataac 1441 actgatagtg ctagtgtaga tcactactag agccaggcat caaataaaac gaaaggctca 1501 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 1561 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagt ccctatcagt 1621 gatagagatt gacatcccta tcagtgatag agatactgag cactactaga gaaagaggag 1681 aaattaagca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg 1741 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag 1801 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc 1861 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac 1921 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag 1981 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc 2041 ttgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac 2101 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc 2161 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac 2221 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc 2281 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa 2341 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa 2401 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 2461 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 2521 tgggcctttc tgcgtttata tactagagca atacgcaaac cgcctctccc cgcgcgttgg 2581 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc 2641 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt 2701 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacata ctagagaaag 2761 aggagaaatt aagcatgtcc agattagata aaagtaaagt gattaacagc gcattagagc 2821 tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg 2881 tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag 2941 ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc 3001 aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg 3061 gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc 3121 aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg 3181 ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag 3241 aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat 3301 tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatatgcg 3361 gattagaaaa acaacttaaa tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt 3421 tagtagctta ataacactga tagtgctagt gtagatcact actagagcca ggcatcaaat 3481 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 3541 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact 3601 agagttgacg gctagctcag tcctaggtac agtgctagct actagagaaa gaggagaaat 3661 taagcatggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct 3721 cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg 3781 aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac 3841 tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc 3901 cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg 3961 tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg 4021 cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg 4081 tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca 4141 tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg 4201 cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc 4261 tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac 4321 gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg 4381 gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg 4441 ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata 4501 ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc 4561 tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca 4621 atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa 4681 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac 4741 tggaaagcgg gcaggctgca aacgacgaaa actacgcttt agtagcttaa taactctgat 4801 agtgctagtg tagatctcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 4861 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 4921 tggctcacct tcgggtgggc ctttctgcgt ttata //