##gff-version 3
##sequence-region BBa_K1520509 1 4955
BBa_K1520509	.	engineered_region	1610	4955	.	+	0	ID=BBa_K1520026;Name=BBa_K1520026
BBa_K1520509	.	engineered_region	3605	4955	.	+	0	ID=BBa_K1520008;Name=BBa_K1520008;Parent=BBa_K1520026
BBa_K1520509	.	terminator	4827	4955	.	+	0	ID=BBa_K1520029;Name=BBa_K1520029;Parent=BBa_K1520008
BBa_K1520509	.	promoter	3605	3639	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K1520008
BBa_K1520509	.	engineered_region	3648	4818	.	+	0	ID=BBa_K1520502;Name=BBa_K1520502;Parent=BBa_K1520008
BBa_K1520509	.	ribosome_entry_site	3648	3665	.	+	0	ID=annotation2384863;Name=rbs;Parent=BBa_K1520502
BBa_K1520509	.	CDS	3666	4818	.	+	0	ID=annotation2384864;Name=lacI;Parent=BBa_K1520502
BBa_K1520509	.	engineered_region	1610	3596	.	+	0	ID=BBa_K1520025;Name=BBa_K1520025;Parent=BBa_K1520026
BBa_K1520509	.	engineered_region	2549	3596	.	+	0	ID=BBa_K1520015;Name=BBa_K1520015;Parent=BBa_K1520025
BBa_K1520509	.	terminator	3468	3596	.	+	0	ID=BBa_K1520029;Name=BBa_K1520029;Parent=BBa_K1520015
BBa_K1520509	.	promoter	2549	2748	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K1520015
BBa_K1520509	.	non_covalent_binding_site	2714	2748	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1520509	.	non_covalent_binding_site	2637	2674	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1520509	.	promoter	2685	2690	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1520509	.	start_codon	2721	2721	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1520509	.	engineered_region	2549	2748	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1520509	.	CDS	2549	2636	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1520509	.	promoter	2709	2714	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1520509	.	engineered_region	2757	3459	.	+	0	ID=BBa_K1520505;Name=BBa_K1520505;Parent=BBa_K1520015
BBa_K1520509	.	ribosome_entry_site	2757	2774	.	+	0	ID=annotation2384877;Name=rbs;Parent=BBa_K1520505
BBa_K1520509	.	CDS	2775	3459	.	+	0	ID=annotation2384878;Name=tetR;Parent=BBa_K1520505
BBa_K1520509	.	engineered_region	1610	2540	.	+	0	ID=BBa_K1520014;Name=BBa_K1520014;Parent=BBa_K1520025
BBa_K1520509	.	engineered_region	1672	2403	.	+	0	ID=BBa_K1520500;Name=BBa_K1520500;Parent=BBa_K1520014
BBa_K1520509	.	terminator	2412	2540	.	+	0	ID=BBa_K1520029;Name=BBa_K1520029;Parent=BBa_K1520014
BBa_K1520509	.	promoter	1610	1663	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K1520014
BBa_K1520509	.	non_covalent_binding_site	1635	1653	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1520509	.	engineered_region	1610	1663	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1520509	.	promoter	1652	1657	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1520509	.	promoter	1629	1634	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1520509	.	non_covalent_binding_site	1610	1628	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1520509	.	terminator	1473	1601	.	+	0	ID=BBa_K1520029;Name=BBa_K1520029
BBa_K1520509	.	engineered_region	762	1464	.	+	0	ID=BBa_K1520505;Name=BBa_K1520505
BBa_K1520509	.	ribosome_entry_site	762	779	.	+	0	ID=annotation2384877;Name=rbs;Parent=BBa_K1520505
BBa_K1520509	.	CDS	780	1464	.	+	0	ID=annotation2384878;Name=tetR;Parent=BBa_K1520505
BBa_K1520509	.	promoter	1	753	.	+	0	ID=BBa_K1520506;Name=BBa_K1520506
BBa_K1520509	.	promoter	554	753	.	+	0	ID=annotation2385509;Name=PgolB;Parent=BBa_K1520506
BBa_K1520509	.	promoter	1	200	.	+	0	ID=annotation2385507;Name=PgolTS;Parent=BBa_K1520506
BBa_K1520509	.	CDS	212	553	.	+	0	ID=annotation2385508;Name=golS;Parent=BBa_K1520506
>BBa_K1520509
acaacgaaggaaaggtcaagcgttcctgacgggtttttacggggcgtgggtcggcatcgtggcgtaaatgtctcgcatc
atcctcttttatgagccatctcacattctcgccgaaccgtgcagcctgaatacgcttgaccttcccacaatggcaagct
ttaggctttctgataccgaatagtcaggatggggaagtcgtcggaggagcgtcatgaacatcggtaaagcagctaaagc
atcgaaagtctcggccaaaatgattcgctactatgaacagattggtctgattcccgcggcaagtcggacggattccggc
tatcgggcctatacccaggctgatgttaatcaattgcattttatacgccgcgcgcgcgacctcggtttttcagttgctg
aaatcagcgacttactgaatctttggaataaccagtcgcggcaaagcgctgacgtcaaacgcctggcgcagacgcacat
tgatgaactggacagacgtatccagaacatgcagcacatggcgcaaaccctcaaagcgctgattcactgctgcgccggc
gacgcgctgccagattgccccattctgcatacgcttggacaacctgacgatagcgagccggaggcgcgtaccggagcgg
tattgcgacgtcctcgtcgccacggactggcaaagcgtctgtaagtcctgagattacgcttgaccttccaacactggca
aggtccagactggcaacagttcccacacaaaaggagttcacttactagagaaagaggagaaattaagcatgtccagatt
agataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactc
gcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagcca
ttgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgc
taaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacag
tatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcg
ctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctac
tactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattc
ggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacg
ctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactga
gcactactagagaaagaggagaaattaagcatggtgagcaagggcgaggaggataacatggccatcatcaaggagttca
tgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgccccta
cgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcag
ttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggct
tcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcga
gttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgg
gaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacg
gcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaa
catcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactcc
accggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagac
tgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctt
tctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgac
aggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctt
tacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggag
aaattaagcatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaag
gtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggc
tttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaa
gattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggta
cacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaa
tgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaa
gaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtg
cagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgc
tgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtg
ctagctactagagaaagaggagaaattaagcatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgcc
ggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaag
cggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgt
tgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgcc
agcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcg
tcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggc
gttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtg
gagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtc
tggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtc
cggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcg
ctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccg
aagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccg
cttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccacc
ctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgac
tggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctct
actagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata