LOCUS BBa_K1520510 4231 bp DNA linear 31-Oct-2024 DEFINITION PgolTS-golS-PgolB-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-g olB-rbs-luxI-Ter ACCESSION BBa_K1520510 VERSION BBa_K1520510.1 FEATURES Location/Qualifiers promoter 1..200 /label=PgolTS CDS 212..553 /label=golS promoter 554..753 /label=PgolB promoter 1..753 /label=BBa_K1520506 RBS 762..779 /label=rbs CDS 780..1422 /label=luxI misc_feature 762..1422 /label=BBa_K1520504 terminator 1431..1559 /label=BBa_K1520029 promoter 1568..1602 /label=BBa_J23100 RBS 1611..1628 /label=rbs CDS 1629..2409 /label=luxR misc_feature 1611..2409 /label=BBa_K1520501 terminator 2418..2546 /label=BBa_K1520029 misc_feature 1568..2546 /label=BBa_K1520003 misc_feature 2555..2574 /label=LuxR/HSL promoter 2574..2579 /label=-35 promoter 2596..2601 /label=-10 misc_feature 2607..2607 /label=start misc_feature 2555..2609 /label=BBa_R0062 promoter 2555..2609 /label=BBa_R0062 misc_feature 2616..2681 /label=singal peptide CDS 2682..3155 /label=OMPA misc_feature 3156..3164 /label=linker CDS 3165..3356 /label=golB misc_feature 3357..3392 /label=flag CDS 2616..3425 /label=BBa_K1520507 RBS 3434..3451 /label=rbs CDS 3452..4094 /label=luxI misc_feature 3434..4094 /label=BBa_K1520504 terminator 4103..4231 /label=BBa_K1520029 ORIGIN 1 acaacgaagg aaaggtcaag cgttcctgac gggtttttac ggggcgtggg tcggcatcgt 61 ggcgtaaatg tctcgcatca tcctctttta tgagccatct cacattctcg ccgaaccgtg 121 cagcctgaat acgcttgacc ttcccacaat ggcaagcttt aggctttctg ataccgaata 181 gtcaggatgg ggaagtcgtc ggaggagcgt catgaacatc ggtaaagcag ctaaagcatc 241 gaaagtctcg gccaaaatga ttcgctacta tgaacagatt ggtctgattc ccgcggcaag 301 tcggacggat tccggctatc gggcctatac ccaggctgat gttaatcaat tgcattttat 361 acgccgcgcg cgcgacctcg gtttttcagt tgctgaaatc agcgacttac tgaatctttg 421 gaataaccag tcgcggcaaa gcgctgacgt caaacgcctg gcgcagacgc acattgatga 481 actggacaga cgtatccaga acatgcagca catggcgcaa accctcaaag cgctgattca 541 ctgctgcgcc ggcgacgcgc tgccagattg ccccattctg catacgcttg gacaacctga 601 cgatagcgag ccggaggcgc gtaccggagc ggtattgcga cgtcctcgtc gccacggact 661 ggcaaagcgt ctgtaagtcc tgagattacg cttgaccttc caacactggc aaggtccaga 721 ctggcaacag ttcccacaca aaaggagttc acttactaga gaaagaggag aaattaagca 781 tgactataat gataaaaaaa tcggattttt tggcaattcc atcggaggag tataaaggta 841 ttctaagtct tcgttatcaa gtgtttaagc aaagacttga gtgggactta gttgtagaaa 901 ataaccttga atcagatgag tatgataact caaatgcaga atatatttat gcttgtgatg 961 atactgaaaa tgtaagtgga tgctggcgtt tattacctac aacaggtgat tatatgctga 1021 aaagtgtttt tcctgaattg cttggtcaac agagtgctcc caaagatcct aatatagtcg 1081 aattaagtcg ttttgctgta ggtaaaaata gctcaaagat aaataactct gctagtgaaa 1141 ttacaatgaa actatttgaa gctatatata aacacgctgt tagtcaaggt attacagaat 1201 atgtaacagt aacatcaaca gcaatagagc gatttttaaa gcgtattaaa gttccttgtc 1261 atcgtattgg agacaaagaa attcatgtat taggtgatac taaatcggtt gtattgtcta 1321 tgcctattaa tgaacagttt aaaaaagcag tcttaaatgc tgcaaacgac gaaaactacg 1381 ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag ccaggcatca 1441 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt 1501 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat 1561 actagagttg acggctagct cagtcctagg tacagtgcta gctactagag aaagaggaga 1621 aattaagcat gaaaaacata aatgccgacg acacatacag aataattaat aaaattaaag 1681 cttgtagaag caataatgat attaatcaat gcttatctga tatgactaaa atggtacatt 1741 gtgaatatta tttactcgcg atcatttatc ctcattctat ggttaaatct gatatttcaa 1801 tcctagataa ttaccctaaa aaatggaggc aatattatga tgacgctaat ttaataaaat 1861 atgatcctat agtagattat tctaactcca atcattcacc aattaattgg aatatatttg 1921 aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa acatcaggtc 1981 ttatcactgg gtttagtttc cctattcata cggctaacaa tggcttcgga atgcttagtt 2041 ttgcacattc agaaaaagac aactatatag atagtttatt tttacatgcg tgtatgaaca 2101 taccattaat tgttccttct ctagttgata attatcgaaa aataaatata gcaaataata 2161 aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt agcgtgggca tgcgaaggaa 2221 aaagctcttg ggatatttca aaaatattag gttgcagtga gcgtactgtc actttccatt 2281 taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt tctaaagcaa 2341 ttttaacagg agcaattgat tgcccatact ttaaaaatta ataacactga tagtgctagt 2401 gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 2461 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 2521 ttcgggtggg cctttctgcg tttatatact agagacctgt aggatcgtac aggtttacgc 2581 aagaaaatgg tttgttatag tcgaataaat actagatgaa aaagacagct atcgcgattg 2641 cagtggcact ggctggtttc gctaccgtag cgcaggccgc ttacccatac gacgtaccag 2701 attacgctgt tgctccgaaa gataacacct ggtacactgg tgctaaactg ggctggtccc 2761 agtaccatga cactggtttc atcaacaaca atggcccgac ccatgaaaac caactgggcg 2821 ctggtgcttt tggtggttac caggttaacc cgtatgttgg ctttgaaatg ggttacgact 2881 ggttaggtcg tatgccgtac aaaggcagcg ttgaaaacgg tgcatacaaa gctcagggcg 2941 ttcaactgac cgctaaactg ggttacccaa tcactgacga cctggacatc tacactcgtc 3001 tgggtggcat ggtatggcgt gcagacacta aatccaacgt ttatggtaaa aaccacgaca 3061 ccggcgtttc tccggtcttc gctggcggtg ttgagtacgc gatcactcct gaaatcgcta 3121 cccgtctgga ataccagtgg accaacaaca tcggtgacgc acacaccatc ggcactcgtc 3181 cggacaacgg agtcgacatg cagttccata ttgatgacat gacctgcggc ggctgcgcca 3241 gtacggtaaa aaagacgatt ctgactctcg atgctaatgc gacggtgaga actgacccgg 3301 cgacgcgtct ggttgacgtt gaaacgtcgc tatccgcgga gcagattgcc gccgccctgc 3361 aaaaggccgg tttcccgccg cgcgagaggc tcgaggatta caaggatgac gacgataaga 3421 tctgatacta gagaaagagg agaaattaag catgactata atgataaaaa aatcggattt 3481 tttggcaatt ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa 3541 gcaaagactt gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa 3601 ctcaaatgca gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg 3661 tttattacct acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca 3721 acagagtgct cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa 3781 tagctcaaag ataaataact ctgctagtga aattacaatg aaactatttg aagctatata 3841 taaacacgct gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga 3901 gcgattttta aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt 3961 attaggtgat actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc 4021 agtcttaaat gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg 4081 ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 4141 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 4201 tcaccttcgg gtgggccttt ctgcgtttat a //