BBa_K1520507 1 BBa_K1520507 OMPA-golB 2014-09-22T11:00:00Z 2015-05-08T01:10:49Z OMPA, golB OMPA-golB A protein to catch gold ions. OMPA present golB outside the membrane and golB will catch gold ions. false false _1902_ 0 21318 9 It's complicated false None false Wei wei annotation2386994 1 linker range2386994 1 541 549 annotation2386995 1 golB range2386995 1 550 741 annotation2386996 1 flag range2386996 1 742 777 annotation2386993 1 OMPA range2386993 1 67 540 annotation2386992 1 singal peptide range2386992 1 1 66 BBa_K1520003 1 BBa_K1520003 Pcons2-rbs-luxR-Ter 2014-09-21T11:00:00Z 2015-05-08T01:10:48Z Pcons2, luxR, Ter Pcons2-rbs-luxR-Ter. It achieves a constant expression of luxR, the AHL combining protein Pcons2 is BBa_J23100 false false _1902_ 0 21318 9 It's complicated false NONE false yue wu component2384888 1 BBa_K1520029 component2384884 1 BBa_J23100 component2384887 1 BBa_K1520501 annotation2384884 1 BBa_J23100 range2384884 1 1 35 annotation2384887 1 BBa_K1520501 range2384887 1 44 842 annotation2384888 1 BBa_K1520029 range2384888 1 851 979 BBa_K1520029 1 BBa_K1520029 Terminator 2014-09-21T11:00:00Z 2015-05-08T01:10:49Z It's designed. It's a terminator with two hairpin. false false _1902_ 0 21318 9 In stock false None false Peiqing Sun BBa_K1520510 1 BBa_K1520510 PgolTS-golS-PgolB-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter 2014-09-22T11:00:00Z 2015-05-08T01:10:49Z PgolTS-golS-PgolB, luxI, Pcons2, luxR, Prlux, OMPA-golB, Ter PgolTS-golS-PgolB-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter It is a test of our positive feedback circuit for gold ions. false false _1902_ 0 21318 9 Not in stock false None false yue wu component2385563 1 BBa_K1520506 component2385589 1 BBa_K1520029 component2385567 1 BBa_K1520029 component2385588 1 BBa_K1520504 component2385566 1 BBa_K1520504 component2385573 1 BBa_K1520003 component2385575 1 BBa_R0062 component2385585 1 BBa_K1520507 annotation2385563 1 BBa_K1520506 range2385563 1 1 753 annotation2385575 1 BBa_R0062 range2385575 1 2555 2609 annotation2385573 1 BBa_K1520003 range2385573 1 1568 2546 annotation2385589 1 BBa_K1520029 range2385589 1 4103 4231 annotation2385585 1 BBa_K1520507 range2385585 1 2616 3425 annotation2385588 1 BBa_K1520504 range2385588 1 3434 4094 annotation2385567 1 BBa_K1520029 range2385567 1 1431 1559 annotation2385566 1 BBa_K1520504 range2385566 1 762 1422 BBa_K1520506 1 BBa_K1520506 PgolTS-golS-PgolB 2014-09-22T11:00:00Z 2015-05-08T01:10:49Z PgolTS, golS, PgolB It is a gold ion detector. Gold ions will cause the CDS after PgolB to be expressed. PgolTS works like a constant promoter, producing gols which is inhibitor of Pgols. Once combining gold ions, gols will stop inhibitor PgolB. So, when there are gold ions, the CDS after this part will be expressed. false false _1902_ 0 21318 9 It's complicated false None false Wei wei annotation2385509 1 PgolB range2385509 1 554 753 annotation2385508 1 golS range2385508 1 212 553 annotation2385507 1 PgolTS range2385507 1 1 200 BBa_K1520501 1 BBa_K1520501 Rbs-luxR(scarless) 2014-09-21T11:00:00Z 2015-05-08T01:10:49Z None Rbs-luxR(scarless) false false _1902_ 0 21318 9 Not in stock false None false yue wu annotation2384862 1 luxR range2384862 1 19 799 annotation2384861 1 rbs range2384861 1 1 18 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_R0062 1 lux pR Promoter (luxR & HSL regulated -- lux pR) 2003-01-31T12:00:00Z 2015-05-08T01:14:15Z <em>V. fischeri</em> Released HQ 2013 Promoter activated by LuxR in concert with HSL</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription. false true _1_ 0 24 7 In stock false <P> <P>This promoter is based on the <em>Vibrio fischeri </em>quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <p><img src="<bb_file>Image1.gif</bb_file>" width="614" height="362"><P> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr annotation2047 1 -10 range2047 1 42 47 annotation2046 1 -35 range2046 1 20 25 annotation7070 1 BBa_R0062 range7070 1 1 55 annotation2048 1 start range2048 1 53 53 annotation2045 1 LuxR/HSL range2045 1 1 20 BBa_K1520504 1 BBa_K1520504 Rbs-luxI(scarless) 2014-09-21T11:00:00Z 2015-05-08T01:10:49Z It is an improvement of luxI(BBa_C0061) by adding a rbs. Rbs-luxI(scarless) It is an improvement of luxI(BBa_C0061) by adding a rbs. false false _1902_ 0 21318 9 Not in stock false None false yue wu annotation2384869 1 rbs range2384869 1 1 18 annotation2384870 1 luxI range2384870 1 19 661 BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_K1520504_sequence 1 aaagaggagaaattaagcatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc BBa_R0062_sequence 1 acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa BBa_K1520507_sequence 1 atgaaaaagacagctatcgcgattgcagtggcactggctggtttcgctaccgtagcgcaggccgctccgaaagataacacctggtacactggtgctaaactgggctggtcccagtaccatgacactggtttcatcaacaacaatggcccgacccatgaaaaccaactgggcgctggtgcttttggtggttaccaggttaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggagtcgacatgcagttccatattgatgacatgacctgcggcggctgcgccagtacggtaaaaaagacgattctgactctcgatgctaatgcgacggtgagaactgacccggcgacgcgtctggttgacgttgaaacgtcgctatccgcggagcagattgccgccgccctgcaaaaggccggtttcccgccgcgcgagaggctcgaggattacaaggatgacgacgataagatctga BBa_K1520501_sequence 1 aaagaggagaaattaagcatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac BBa_K1520506_sequence 1 acaacgaaggaaaggtcaagcgttcctgacgggtttttacggggcgtgggtcggcatcgtggcgtaaatgtctcgcatcatcctcttttatgagccatctcacattctcgccgaaccgtgcagcctgaatacgcttgaccttcccacaatggcaagctttaggctttctgataccgaatagtcaggatggggaagtcgtcggaggagcgtcatgaacatcggtaaagcagctaaagcatcgaaagtctcggccaaaatgattcgctactatgaacagattggtctgattcccgcggcaagtcggacggattccggctatcgggcctatacccaggctgatgttaatcaattgcattttatacgccgcgcgcgcgacctcggtttttcagttgctgaaatcagcgacttactgaatctttggaataaccagtcgcggcaaagcgctgacgtcaaacgcctggcgcagacgcacattgatgaactggacagacgtatccagaacatgcagcacatggcgcaaaccctcaaagcgctgattcactgctgcgccggcgacgcgctgccagattgccccattctgcatacgcttggacaacctgacgatagcgagccggaggcgcgtaccggagcggtattgcgacgtcctcgtcgccacggactggcaaagcgtctgtaagtcctgagattacgcttgaccttccaacactggcaaggtccagactggcaacagttcccacacaaaaggagttcact BBa_K1520003_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaattaagcatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1520510_sequence 1 acaacgaaggaaaggtcaagcgttcctgacgggtttttacggggcgtgggtcggcatcgtggcgtaaatgtctcgcatcatcctcttttatgagccatctcacattctcgccgaaccgtgcagcctgaatacgcttgaccttcccacaatggcaagctttaggctttctgataccgaatagtcaggatggggaagtcgtcggaggagcgtcatgaacatcggtaaagcagctaaagcatcgaaagtctcggccaaaatgattcgctactatgaacagattggtctgattcccgcggcaagtcggacggattccggctatcgggcctatacccaggctgatgttaatcaattgcattttatacgccgcgcgcgcgacctcggtttttcagttgctgaaatcagcgacttactgaatctttggaataaccagtcgcggcaaagcgctgacgtcaaacgcctggcgcagacgcacattgatgaactggacagacgtatccagaacatgcagcacatggcgcaaaccctcaaagcgctgattcactgctgcgccggcgacgcgctgccagattgccccattctgcatacgcttggacaacctgacgatagcgagccggaggcgcgtaccggagcggtattgcgacgtcctcgtcgccacggactggcaaagcgtctgtaagtcctgagattacgcttgaccttccaacactggcaaggtccagactggcaacagttcccacacaaaaggagttcacttactagagaaagaggagaaattaagcatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaattaagcatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagatgaaaaagacagctatcgcgattgcagtggcactggctggtttcgctaccgtagcgcaggccgcttacccatacgacgtaccagattacgctgttgctccgaaagataacacctggtacactggtgctaaactgggctggtcccagtaccatgacactggtttcatcaacaacaatggcccgacccatgaaaaccaactgggcgctggtgcttttggtggttaccaggttaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggagtcgacatgcagttccatattgatgacatgacctgcggcggctgcgccagtacggtaaaaaagacgattctgactctcgatgctaatgcgacggtgagaactgacccggcgacgcgtctggttgacgttgaaacgtcgctatccgcggagcagattgccgccgccctgcaaaaggccggtttcccgccgcgcgagaggctcgaggattacaaggatgacgacgataagatctgatactagagaaagaggagaaattaagcatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1520029_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z