LOCUS BBa_K1520513 4335 bp DNA linear 22-Nov-2024 DEFINITION MC7-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lac I-Ter ACCESSION BBa_K1520513 VERSION BBa_K1520513.1 FEATURES Location/Qualifiers promoter 1..133 /label=BBa_K1520001 RBS 142..159 /label=rbs CDS 160..844 /label=tetR misc_feature 142..844 /label=BBa_K1520505 terminator 853..981 /label=BBa_K1520029 misc_feature 990..1008 /label=TetR 1 misc_feature 990..1043 /label=BBa_R0040 promoter 1009..1014 /label=-35 misc_feature 1015..1033 /label=TetR 2 promoter 1032..1037 /label=-10 promoter 990..1043 /label=BBa_R0040 misc_feature 1052..1783 /label=BBa_K1520500 terminator 1792..1920 /label=BBa_K1520029 misc_feature 990..1920 /label=BBa_K1520014 CDS 1929..2016 /label=end of LacI coding region (inactive) misc_feature 1929..2128 /label=BBa_R0010 misc_feature 2017..2054 /label=CAP binding site promoter 2065..2070 /label=-35 promoter 2089..2094 /label=-10 misc_feature 2094..2128 /label=LacI binding site misc_feature 2101..2101 /label=start promoter 1929..2128 /label=BBa_R0010 RBS 2137..2154 /label=rbs CDS 2155..2839 /label=tetR misc_feature 2137..2839 /label=BBa_K1520505 terminator 2848..2976 /label=BBa_K1520029 misc_feature 1929..2976 /label=BBa_K1520015 misc_feature 990..2976 /label=BBa_K1520025 promoter 2985..3019 /label=BBa_J23100 RBS 3028..3045 /label=rbs CDS 3046..4198 /label=lacI misc_feature 3028..4198 /label=BBa_K1520502 terminator 4207..4335 /label=BBa_K1520029 misc_feature 2985..4335 /label=BBa_K1520008 misc_feature 990..4335 /label=BBa_K1520026 ORIGIN 1 aaacaaacaa agctgtcacc ggatgtgctt tccggtctga tgagtccgtg tccggtggac 61 agaaaagggg aatagtggaa gacgtgtatc gcgggacggg acgaggacga aacagcaaaa 121 agaaaaataa aaatactaga gaaagaggag aaattaagca tgtccagatt agataaaagt 181 aaagtgatta acagcgcatt agagctgctt aatgaggtcg gaatcgaagg tttaacaacc 241 cgtaaactcg cccagaagct aggtgtagag cagcctacat tgtattggca tgtaaaaaat 301 aagcgggctt tgctcgacgc cttagccatt gagatgttag ataggcacca tactcacttt 361 tgccctttag aaggggaaag ctggcaagat tttttacgta ataacgctaa aagttttaga 421 tgtgctttac taagtcatcg cgatggagca aaagtacatt taggtacacg gcctacagaa 481 aaacagtatg aaactctcga aaatcaatta gcctttttat gccaacaagg tttttcacta 541 gagaatgcat tatatgcact cagcgctgtg gggcatttta ctttaggttg cgtattggaa 601 gatcaagagc atcaagtcgc taaagaagaa agggaaacac ctactactga tagtatgccg 661 ccattattac gacaagctat cgaattattt gatcaccaag gtgcagagcc agccttctta 721 ttcggccttg aattgatcat atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc 781 gctgcaaacg acgaaaacta cgctttagta gcttaataac actgatagtg ctagtgtaga 841 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 961 gtgggccttt ctgcgtttat atactagagt ccctatcagt gatagagatt gacatcccta 1021 tcagtgatag agatactgag cactactaga gaaagaggag aaattaagca tggtgagcaa 1081 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga 1141 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga 1201 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga 1261 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat 1321 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt 1381 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ttgcaggacg gcgagttcat 1441 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa 1501 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg 1561 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac 1621 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt 1681 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg 1741 ccgccactcc accggcggca tggacgagct gtacaagtaa taatactaga gccaggcatc 1801 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 1861 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 1921 tactagagca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg 1981 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta 2041 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg 2101 aattgtgagc ggataacaat ttcacacata ctagagaaag aggagaaatt aagcatgtcc 2161 agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc 2221 gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat 2281 tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg 2341 caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac 2401 gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt 2461 acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa 2521 caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta 2581 ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact 2641 actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca 2701 gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa 2761 tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt tagtagctta ataacactga 2821 tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga 2881 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 2941 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagttgacg gctagctcag 3001 tcctaggtac agtgctagct actagagaaa gaggagaaat taagcatggt gaatgtgaaa 3061 ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc 3121 gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg 3181 gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg 3241 ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg 3301 attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc 3361 ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg 3421 atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat 3481 gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc 3541 catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc 3601 gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat 3661 aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc 3721 atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg 3781 ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg 3841 cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat 3901 atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac 3961 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca 4021 ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 4081 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcaggctgca 4141 aacgacgaaa actacgcttt agtagcttaa taactctgat agtgctagtg tagatctcta 4201 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 4261 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 4321 ctttctgcgt ttata //