##gff-version 3
##sequence-region BBa_K1520513 1 4335
BBa_K1520513	.	engineered_region	990	4335	.	+	0	ID=BBa_K1520026;Name=BBa_K1520026
BBa_K1520513	.	engineered_region	990	2976	.	+	0	ID=BBa_K1520025;Name=BBa_K1520025;Parent=BBa_K1520026
BBa_K1520513	.	engineered_region	1929	2976	.	+	0	ID=BBa_K1520015;Name=BBa_K1520015;Parent=BBa_K1520025
BBa_K1520513	.	promoter	1929	2128	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K1520015
BBa_K1520513	.	CDS	1929	2016	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1520513	.	promoter	2089	2094	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1520513	.	non_covalent_binding_site	2094	2128	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1520513	.	engineered_region	1929	2128	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1520513	.	promoter	2065	2070	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1520513	.	non_covalent_binding_site	2017	2054	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1520513	.	start_codon	2101	2101	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1520513	.	terminator	2848	2976	.	+	0	ID=BBa_K1520029;Name=BBa_K1520029;Parent=BBa_K1520015
BBa_K1520513	.	engineered_region	2137	2839	.	+	0	ID=BBa_K1520505;Name=BBa_K1520505;Parent=BBa_K1520015
BBa_K1520513	.	CDS	2155	2839	.	+	0	ID=annotation2384878;Name=tetR;Parent=BBa_K1520505
BBa_K1520513	.	ribosome_entry_site	2137	2154	.	+	0	ID=annotation2384877;Name=rbs;Parent=BBa_K1520505
BBa_K1520513	.	engineered_region	990	1920	.	+	0	ID=BBa_K1520014;Name=BBa_K1520014;Parent=BBa_K1520025
BBa_K1520513	.	engineered_region	1052	1783	.	+	0	ID=BBa_K1520500;Name=BBa_K1520500;Parent=BBa_K1520014
BBa_K1520513	.	promoter	990	1043	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K1520014
BBa_K1520513	.	engineered_region	990	1043	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1520513	.	promoter	1009	1014	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1520513	.	non_covalent_binding_site	1015	1033	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1520513	.	promoter	1032	1037	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1520513	.	non_covalent_binding_site	990	1008	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1520513	.	terminator	1792	1920	.	+	0	ID=BBa_K1520029;Name=BBa_K1520029;Parent=BBa_K1520014
BBa_K1520513	.	engineered_region	2985	4335	.	+	0	ID=BBa_K1520008;Name=BBa_K1520008;Parent=BBa_K1520026
BBa_K1520513	.	engineered_region	3028	4198	.	+	0	ID=BBa_K1520502;Name=BBa_K1520502;Parent=BBa_K1520008
BBa_K1520513	.	CDS	3046	4198	.	+	0	ID=annotation2384864;Name=lacI;Parent=BBa_K1520502
BBa_K1520513	.	ribosome_entry_site	3028	3045	.	+	0	ID=annotation2384863;Name=rbs;Parent=BBa_K1520502
BBa_K1520513	.	promoter	2985	3019	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K1520008
BBa_K1520513	.	terminator	4207	4335	.	+	0	ID=BBa_K1520029;Name=BBa_K1520029;Parent=BBa_K1520008
BBa_K1520513	.	engineered_region	142	844	.	+	0	ID=BBa_K1520505;Name=BBa_K1520505
BBa_K1520513	.	CDS	160	844	.	+	0	ID=annotation2384878;Name=tetR;Parent=BBa_K1520505
BBa_K1520513	.	ribosome_entry_site	142	159	.	+	0	ID=annotation2384877;Name=rbs;Parent=BBa_K1520505
BBa_K1520513	.	promoter	1	133	.	+	0	ID=BBa_K1520001;Name=BBa_K1520001
BBa_K1520513	.	terminator	853	981	.	+	0	ID=BBa_K1520029;Name=BBa_K1520029
>BBa_K1520513
aaacaaacaaagctgtcaccggatgtgctttccggtctgatgagtccgtgtccggtggacagaaaaggggaatagtgga
agacgtgtatcgcgggacgggacgaggacgaaacagcaaaaagaaaaataaaaatactagagaaagaggagaaattaag
catgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaaca
acccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcg
acgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagatttttt
acgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcct
acagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattat
atgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaag
ggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagcca
gccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacg
acgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtga
tagagatactgagcactactagagaaagaggagaaattaagcatggtgagcaagggcgaggaggataacatggccatca
tcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcga
gggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatc
ctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtcct
tccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctcctt
gcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaag
accatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctga
agctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgc
ctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgag
ggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggct
cagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacctt
cgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgc
agctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattagg
caccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatacta
gagaaagaggagaaattaagcatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgagg
tcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaa
aaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggg
gaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaag
tacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggttt
ttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcat
caagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttg
atcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtga
aagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtc
ctaggtacagtgctagctactagagaaagaggagaaattaagcatggtgaatgtgaaaccagtaacgttatacgatgtc
gcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcggg
aaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgtt
gctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgat
caactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttc
tcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcac
taatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacg
cgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcgg
cgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactg
gagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaac
gatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggat
acgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaac
cagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaa
agaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgac
aggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgcta
gtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata