##gff-version 3
##sequence-region BBa_K1520514 1 4197
BBa_K1520514	.	engineered_region	852	4197	.	+	0	ID=BBa_K1520026;Name=BBa_K1520026
BBa_K1520514	.	engineered_region	852	2838	.	+	0	ID=BBa_K1520025;Name=BBa_K1520025;Parent=BBa_K1520026
BBa_K1520514	.	engineered_region	852	1782	.	+	0	ID=BBa_K1520014;Name=BBa_K1520014;Parent=BBa_K1520025
BBa_K1520514	.	promoter	852	905	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K1520014
BBa_K1520514	.	promoter	871	876	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1520514	.	non_covalent_binding_site	877	895	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1520514	.	engineered_region	852	905	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1520514	.	non_covalent_binding_site	852	870	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1520514	.	promoter	894	899	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1520514	.	engineered_region	914	1645	.	+	0	ID=BBa_K1520500;Name=BBa_K1520500;Parent=BBa_K1520014
BBa_K1520514	.	terminator	1654	1782	.	+	0	ID=BBa_K1520029;Name=BBa_K1520029;Parent=BBa_K1520014
BBa_K1520514	.	engineered_region	1791	2838	.	+	0	ID=BBa_K1520015;Name=BBa_K1520015;Parent=BBa_K1520025
BBa_K1520514	.	engineered_region	1999	2701	.	+	0	ID=BBa_K1520505;Name=BBa_K1520505;Parent=BBa_K1520015
BBa_K1520514	.	CDS	2017	2701	.	+	0	ID=annotation2384878;Name=tetR;Parent=BBa_K1520505
BBa_K1520514	.	ribosome_entry_site	1999	2016	.	+	0	ID=annotation2384877;Name=rbs;Parent=BBa_K1520505
BBa_K1520514	.	terminator	2710	2838	.	+	0	ID=BBa_K1520029;Name=BBa_K1520029;Parent=BBa_K1520015
BBa_K1520514	.	promoter	1791	1990	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K1520015
BBa_K1520514	.	non_covalent_binding_site	1956	1990	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1520514	.	non_covalent_binding_site	1879	1916	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1520514	.	engineered_region	1791	1990	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1520514	.	promoter	1927	1932	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1520514	.	CDS	1791	1878	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1520514	.	start_codon	1963	1963	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1520514	.	promoter	1951	1956	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1520514	.	engineered_region	2847	4197	.	+	0	ID=BBa_K1520008;Name=BBa_K1520008;Parent=BBa_K1520026
BBa_K1520514	.	engineered_region	2890	4060	.	+	0	ID=BBa_K1520502;Name=BBa_K1520502;Parent=BBa_K1520008
BBa_K1520514	.	ribosome_entry_site	2890	2907	.	+	0	ID=annotation2384863;Name=rbs;Parent=BBa_K1520502
BBa_K1520514	.	CDS	2908	4060	.	+	0	ID=annotation2384864;Name=lacI;Parent=BBa_K1520502
BBa_K1520514	.	terminator	4069	4197	.	+	0	ID=BBa_K1520029;Name=BBa_K1520029;Parent=BBa_K1520008
BBa_K1520514	.	promoter	2847	2881	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K1520008
BBa_K1520514	.	promoter	1	132	.	+	0	ID=BBa_K1520002;Name=BBa_K1520002
BBa_K1520514	.	engineered_region	141	843	.	+	0	ID=BBa_K1520505;Name=BBa_K1520505
BBa_K1520514	.	CDS	159	843	.	+	0	ID=annotation2384878;Name=tetR;Parent=BBa_K1520505
BBa_K1520514	.	ribosome_entry_site	141	158	.	+	0	ID=annotation2384877;Name=rbs;Parent=BBa_K1520505
>BBa_K1520514
aaacaaacaaagctgtcaccggatgtgctttccggtctgatgagtccgtgtccgggagtgcgtgcacctgcacctgggg
gggtgttcgcttgggacgggacgaggacgaaacagcaaaaagaaaaataaaaatactagagaaagaggagaaattaagc
atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaa
cccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcga
cgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagatttttta
cgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggccta
cagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattata
tgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagg
gaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccag
ccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacga
cgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtccctatcagtgatagag
attgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaattaagcatggtgagcaagggcga
ggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttc
gagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccc
tgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccc
cgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgacc
gtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacg
gccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaaggg
cgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaag
cccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtgg
aacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgc
gttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgt
gagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgga
taacaatttcacacatactagagaaagaggagaaattaagcatgtccagattagataaaagtaaagtgattaacagcgc
attagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcct
acattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactc
acttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaag
tcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcc
tttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcg
tattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacg
acaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggatta
gaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagt
gctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatc
tgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactag
agttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaattaagcatggtgaatgtgaaac
cagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagcca
cgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaa
ctggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcgg
cgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaa
agcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccatt
gctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattt
tctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcggg
cccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgata
gcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttccca
ctgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgc
ggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggat
tttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgt
tgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattc
attaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagct
taataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactg
ggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttc
tgcgtttata