LOCUS BBa_K1520515 3611 bp DNA linear 28-Nov-2024 DEFINITION MC7-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-T er. ACCESSION BBa_K1520515 VERSION BBa_K1520515.1 FEATURES Location/Qualifiers promoter 1..133 /label=BBa_K1520001 RBS 142..159 /label=rbs CDS 160..802 /label=luxI misc_feature 142..802 /label=BBa_K1520504 terminator 811..939 /label=BBa_K1520029 promoter 948..982 /label=BBa_J23100 RBS 991..1008 /label=rbs CDS 1009..1789 /label=luxR misc_feature 991..1789 /label=BBa_K1520501 terminator 1798..1926 /label=BBa_K1520029 misc_feature 948..1926 /label=BBa_K1520003 misc_feature 1935..1954 /label=LuxR/HSL promoter 1954..1959 /label=-35 promoter 1976..1981 /label=-10 misc_feature 1987..1987 /label=start misc_feature 1935..1989 /label=BBa_R0062 promoter 1935..1989 /label=BBa_R0062 misc_feature 1996..2061 /label=singal peptide CDS 2062..2535 /label=OMPA misc_feature 2536..2544 /label=linker CDS 2545..2736 /label=golB misc_feature 2737..2772 /label=flag CDS 1996..2805 /label=BBa_K1520507 RBS 2814..2831 /label=rbs CDS 2832..3474 /label=luxI misc_feature 2814..3474 /label=BBa_K1520504 terminator 3483..3611 /label=BBa_K1520029 ORIGIN 1 aaacaaacaa agctgtcacc ggatgtgctt tccggtctga tgagtccgtg tccggtggac 61 agaaaagggg aatagtggaa gacgtgtatc gcgggacggg acgaggacga aacagcaaaa 121 agaaaaataa aaatactaga gaaagaggag aaattaagca tgactataat gataaaaaaa 181 tcggattttt tggcaattcc atcggaggag tataaaggta ttctaagtct tcgttatcaa 241 gtgtttaagc aaagacttga gtgggactta gttgtagaaa ataaccttga atcagatgag 301 tatgataact caaatgcaga atatatttat gcttgtgatg atactgaaaa tgtaagtgga 361 tgctggcgtt tattacctac aacaggtgat tatatgctga aaagtgtttt tcctgaattg 421 cttggtcaac agagtgctcc caaagatcct aatatagtcg aattaagtcg ttttgctgta 481 ggtaaaaata gctcaaagat aaataactct gctagtgaaa ttacaatgaa actatttgaa 541 gctatatata aacacgctgt tagtcaaggt attacagaat atgtaacagt aacatcaaca 601 gcaatagagc gatttttaaa gcgtattaaa gttccttgtc atcgtattgg agacaaagaa 661 attcatgtat taggtgatac taaatcggtt gtattgtcta tgcctattaa tgaacagttt 721 aaaaaagcag tcttaaatgc tgcaaacgac gaaaactacg ctttagtagc ttaataactc 781 tgatagtgct agtgtagatc tctactagag ccaggcatca aataaaacga aaggctcagt 841 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 901 acactggctc accttcgggt gggcctttct gcgtttatat actagagttg acggctagct 961 cagtcctagg tacagtgcta gctactagag aaagaggaga aattaagcat gaaaaacata 1021 aatgccgacg acacatacag aataattaat aaaattaaag cttgtagaag caataatgat 1081 attaatcaat gcttatctga tatgactaaa atggtacatt gtgaatatta tttactcgcg 1141 atcatttatc ctcattctat ggttaaatct gatatttcaa tcctagataa ttaccctaaa 1201 aaatggaggc aatattatga tgacgctaat ttaataaaat atgatcctat agtagattat 1261 tctaactcca atcattcacc aattaattgg aatatatttg aaaacaatgc tgtaaataaa 1321 aaatctccaa atgtaattaa agaagcgaaa acatcaggtc ttatcactgg gtttagtttc 1381 cctattcata cggctaacaa tggcttcgga atgcttagtt ttgcacattc agaaaaagac 1441 aactatatag atagtttatt tttacatgcg tgtatgaaca taccattaat tgttccttct 1501 ctagttgata attatcgaaa aataaatata gcaaataata aatcaaacaa cgatttaacc 1561 aaaagagaaa aagaatgttt agcgtgggca tgcgaaggaa aaagctcttg ggatatttca 1621 aaaatattag gttgcagtga gcgtactgtc actttccatt taaccaatgc gcaaatgaaa 1681 ctcaatacaa caaaccgctg ccaaagtatt tctaaagcaa ttttaacagg agcaattgat 1741 tgcccatact ttaaaaatta ataacactga tagtgctagt gtagatcact actagagcca 1801 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 1861 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 1921 tttatatact agagacctgt aggatcgtac aggtttacgc aagaaaatgg tttgttatag 1981 tcgaataaat actagatgaa aaagacagct atcgcgattg cagtggcact ggctggtttc 2041 gctaccgtag cgcaggccgc ttacccatac gacgtaccag attacgctgt tgctccgaaa 2101 gataacacct ggtacactgg tgctaaactg ggctggtccc agtaccatga cactggtttc 2161 atcaacaaca atggcccgac ccatgaaaac caactgggcg ctggtgcttt tggtggttac 2221 caggttaacc cgtatgttgg ctttgaaatg ggttacgact ggttaggtcg tatgccgtac 2281 aaaggcagcg ttgaaaacgg tgcatacaaa gctcagggcg ttcaactgac cgctaaactg 2341 ggttacccaa tcactgacga cctggacatc tacactcgtc tgggtggcat ggtatggcgt 2401 gcagacacta aatccaacgt ttatggtaaa aaccacgaca ccggcgtttc tccggtcttc 2461 gctggcggtg ttgagtacgc gatcactcct gaaatcgcta cccgtctgga ataccagtgg 2521 accaacaaca tcggtgacgc acacaccatc ggcactcgtc cggacaacgg agtcgacatg 2581 cagttccata ttgatgacat gacctgcggc ggctgcgcca gtacggtaaa aaagacgatt 2641 ctgactctcg atgctaatgc gacggtgaga actgacccgg cgacgcgtct ggttgacgtt 2701 gaaacgtcgc tatccgcgga gcagattgcc gccgccctgc aaaaggccgg tttcccgccg 2761 cgcgagaggc tcgaggatta caaggatgac gacgataaga tctgatacta gagaaagagg 2821 agaaattaag catgactata atgataaaaa aatcggattt tttggcaatt ccatcggagg 2881 agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt gagtgggact 2941 tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca gaatatattt 3001 atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct acaacaggtg 3061 attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct cccaaagatc 3121 ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag ataaataact 3181 ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct gttagtcaag 3241 gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta aagcgtatta 3301 aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat actaaatcgg 3361 ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat gctgcaaacg 3421 acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag 3481 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 3541 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 3601 ctgcgtttat a //