MC7, luxI, Pcons2, luxR, Prlux, OMPA-golB, Ter
MC7-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter. It is a test of our positive feedback circuit for microcystin.
Promoter (luxR & HSL regulated -- lux pR)
Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
The aptamers are from results of paper, In vitro selection of specific aptamers against microcysitin-LR. The whole riboswitches are designed.
It is a riboswitch designed by our team. We design a lot of riboswitch for microcystin, and then choose two excellent ones, MC7 and MC31, by modeling. They are riboswitches based on the stability of mRNA. The structure of them include a hammerhead ribozyme and a microcystin aptamer. Users can connect them down the promoter to use them.
We tested a lot of riboswitches by model and experiment to choose better one.
Pcons2-rbs-luxR-Ter. It achieves a constant expression of luxR, the AHL combining protein Pcons2 is BBa_J23100
It is an improvement of luxI(BBa_C0061) by adding a rbs.
Rbs-luxI(scarless) It is an improvement of luxI(BBa_C0061) by adding a rbs.
OMPA-golB A protein to catch gold ions. OMPA present golB outside the membrane and golB will catch gold ions.
Conversion of the iGEM parts registry to SBOL2.1