LOCUS BBa_K1520516 3610 bp DNA linear 22-Nov-2024 DEFINITION MC31-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI- Ter ACCESSION BBa_K1520516 VERSION BBa_K1520516.1 FEATURES Location/Qualifiers promoter 1..132 /label=BBa_K1520002 RBS 141..158 /label=rbs CDS 159..801 /label=luxI misc_feature 141..801 /label=BBa_K1520504 terminator 810..938 /label=BBa_K1520029 promoter 947..981 /label=BBa_J23100 RBS 990..1007 /label=rbs CDS 1008..1788 /label=luxR misc_feature 990..1788 /label=BBa_K1520501 terminator 1797..1925 /label=BBa_K1520029 misc_feature 947..1925 /label=BBa_K1520003 misc_feature 1934..1953 /label=LuxR/HSL promoter 1953..1958 /label=-35 promoter 1975..1980 /label=-10 misc_feature 1986..1986 /label=start misc_feature 1934..1988 /label=BBa_R0062 promoter 1934..1988 /label=BBa_R0062 misc_feature 1995..2060 /label=singal peptide CDS 2061..2534 /label=OMPA misc_feature 2535..2543 /label=linker CDS 2544..2735 /label=golB misc_feature 2736..2771 /label=flag CDS 1995..2804 /label=BBa_K1520507 RBS 2813..2830 /label=rbs CDS 2831..3473 /label=luxI misc_feature 2813..3473 /label=BBa_K1520504 terminator 3482..3610 /label=BBa_K1520029 ORIGIN 1 aaacaaacaa agctgtcacc ggatgtgctt tccggtctga tgagtccgtg tccgggagtg 61 cgtgcacctg cacctggggg ggtgttcgct tgggacggga cgaggacgaa acagcaaaaa 121 gaaaaataaa aatactagag aaagaggaga aattaagcat gactataatg ataaaaaaat 181 cggatttttt ggcaattcca tcggaggagt ataaaggtat tctaagtctt cgttatcaag 241 tgtttaagca aagacttgag tgggacttag ttgtagaaaa taaccttgaa tcagatgagt 301 atgataactc aaatgcagaa tatatttatg cttgtgatga tactgaaaat gtaagtggat 361 gctggcgttt attacctaca acaggtgatt atatgctgaa aagtgttttt cctgaattgc 421 ttggtcaaca gagtgctccc aaagatccta atatagtcga attaagtcgt tttgctgtag 481 gtaaaaatag ctcaaagata aataactctg ctagtgaaat tacaatgaaa ctatttgaag 541 ctatatataa acacgctgtt agtcaaggta ttacagaata tgtaacagta acatcaacag 601 caatagagcg atttttaaag cgtattaaag ttccttgtca tcgtattgga gacaaagaaa 661 ttcatgtatt aggtgatact aaatcggttg tattgtctat gcctattaat gaacagttta 721 aaaaagcagt cttaaatgct gcaaacgacg aaaactacgc tttagtagct taataactct 781 gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa aggctcagtc 841 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 901 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagttga cggctagctc 961 agtcctaggt acagtgctag ctactagaga aagaggagaa attaagcatg aaaaacataa 1021 atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc aataatgata 1081 ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat ttactcgcga 1141 tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat taccctaaaa 1201 aatggaggca atattatgat gacgctaatt taataaaata tgatcctata gtagattatt 1261 ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct gtaaataaaa 1321 aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg tttagtttcc 1381 ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca gaaaaagaca 1441 actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt gttccttctc 1501 tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac gatttaacca 1561 aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg gatatttcaa 1621 aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg caaatgaaac 1681 tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga gcaattgatt 1741 gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta ctagagccag 1801 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 1861 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 1921 ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt ttgttatagt 1981 cgaataaata ctagatgaaa aagacagcta tcgcgattgc agtggcactg gctggtttcg 2041 ctaccgtagc gcaggccgct tacccatacg acgtaccaga ttacgctgtt gctccgaaag 2101 ataacacctg gtacactggt gctaaactgg gctggtccca gtaccatgac actggtttca 2161 tcaacaacaa tggcccgacc catgaaaacc aactgggcgc tggtgctttt ggtggttacc 2221 aggttaaccc gtatgttggc tttgaaatgg gttacgactg gttaggtcgt atgccgtaca 2281 aaggcagcgt tgaaaacggt gcatacaaag ctcagggcgt tcaactgacc gctaaactgg 2341 gttacccaat cactgacgac ctggacatct acactcgtct gggtggcatg gtatggcgtg 2401 cagacactaa atccaacgtt tatggtaaaa accacgacac cggcgtttct ccggtcttcg 2461 ctggcggtgt tgagtacgcg atcactcctg aaatcgctac ccgtctggaa taccagtgga 2521 ccaacaacat cggtgacgca cacaccatcg gcactcgtcc ggacaacgga gtcgacatgc 2581 agttccatat tgatgacatg acctgcggcg gctgcgccag tacggtaaaa aagacgattc 2641 tgactctcga tgctaatgcg acggtgagaa ctgacccggc gacgcgtctg gttgacgttg 2701 aaacgtcgct atccgcggag cagattgccg ccgccctgca aaaggccggt ttcccgccgc 2761 gcgagaggct cgaggattac aaggatgacg acgataagat ctgatactag agaaagagga 2821 gaaattaagc atgactataa tgataaaaaa atcggatttt ttggcaattc catcggagga 2881 gtataaaggt attctaagtc ttcgttatca agtgtttaag caaagacttg agtgggactt 2941 agttgtagaa aataaccttg aatcagatga gtatgataac tcaaatgcag aatatattta 3001 tgcttgtgat gatactgaaa atgtaagtgg atgctggcgt ttattaccta caacaggtga 3061 ttatatgctg aaaagtgttt ttcctgaatt gcttggtcaa cagagtgctc ccaaagatcc 3121 taatatagtc gaattaagtc gttttgctgt aggtaaaaat agctcaaaga taaataactc 3181 tgctagtgaa attacaatga aactatttga agctatatat aaacacgctg ttagtcaagg 3241 tattacagaa tatgtaacag taacatcaac agcaatagag cgatttttaa agcgtattaa 3301 agttccttgt catcgtattg gagacaaaga aattcatgta ttaggtgata ctaaatcggt 3361 tgtattgtct atgcctatta atgaacagtt taaaaaagca gtcttaaatg ctgcaaacga 3421 cgaaaactac gctttagtag cttaataact ctgatagtgc tagtgtagat ctctactaga 3481 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 3541 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 3601 tgcgtttata //