BBa_R0062 1 lux pR Promoter (luxR & HSL regulated -- lux pR) 2003-01-31T12:00:00Z 2015-05-08T01:14:15Z <em>V. fischeri</em> Released HQ 2013 Promoter activated by LuxR in concert with HSL</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription. false true _1_ 0 24 7 In stock false <P> <P>This promoter is based on the <em>Vibrio fischeri </em>quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <p><img src="<bb_file>Image1.gif</bb_file>" width="614" height="362"><P> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr annotation2048 1 start range2048 1 53 53 annotation2047 1 -10 range2047 1 42 47 annotation2046 1 -35 range2046 1 20 25 annotation7070 1 BBa_R0062 range7070 1 1 55 annotation2045 1 LuxR/HSL range2045 1 1 20 BBa_K1520507 1 BBa_K1520507 OMPA-golB 2014-09-22T11:00:00Z 2015-05-08T01:10:49Z OMPA, golB OMPA-golB A protein to catch gold ions. OMPA present golB outside the membrane and golB will catch gold ions. false false _1902_ 0 21318 9 It's complicated false None false Wei wei annotation2386995 1 golB range2386995 1 550 741 annotation2386996 1 flag range2386996 1 742 777 annotation2386992 1 singal peptide range2386992 1 1 66 annotation2386994 1 linker range2386994 1 541 549 annotation2386993 1 OMPA range2386993 1 67 540 BBa_K1520501 1 BBa_K1520501 Rbs-luxR(scarless) 2014-09-21T11:00:00Z 2015-05-08T01:10:49Z None Rbs-luxR(scarless) false false _1902_ 0 21318 9 Not in stock false None false yue wu annotation2384862 1 luxR range2384862 1 19 799 annotation2384861 1 rbs range2384861 1 1 18 BBa_K1520003 1 BBa_K1520003 Pcons2-rbs-luxR-Ter 2014-09-21T11:00:00Z 2015-05-08T01:10:48Z Pcons2, luxR, Ter Pcons2-rbs-luxR-Ter. It achieves a constant expression of luxR, the AHL combining protein Pcons2 is BBa_J23100 false false _1902_ 0 21318 9 It's complicated false NONE false yue wu component2384884 1 BBa_J23100 component2384887 1 BBa_K1520501 component2384888 1 BBa_K1520029 annotation2384887 1 BBa_K1520501 range2384887 1 44 842 annotation2384888 1 BBa_K1520029 range2384888 1 851 979 annotation2384884 1 BBa_J23100 range2384884 1 1 35 BBa_K1520516 1 BBa_K1520516 MC31-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter 2014-09-22T11:00:00Z 2015-05-08T01:10:49Z MC31, luxI, Pcons2, luxR, Prlux, OMPA-golB, Ter MC31-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter It is a test of our positive feedback circuit for microcystin. false false _1902_ 0 21318 9 Not in stock false None false yue wu component2385714 1 BBa_K1520507 component2385692 1 BBa_K1520002 component2385702 1 BBa_K1520003 component2385696 1 BBa_K1520029 component2385717 1 BBa_K1520504 component2385704 1 BBa_R0062 component2385718 1 BBa_K1520029 component2385695 1 BBa_K1520504 annotation2385704 1 BBa_R0062 range2385704 1 1934 1988 annotation2385695 1 BBa_K1520504 range2385695 1 141 801 annotation2385714 1 BBa_K1520507 range2385714 1 1995 2804 annotation2385702 1 BBa_K1520003 range2385702 1 947 1925 annotation2385696 1 BBa_K1520029 range2385696 1 810 938 annotation2385717 1 BBa_K1520504 range2385717 1 2813 3473 annotation2385692 1 BBa_K1520002 range2385692 1 1 132 annotation2385718 1 BBa_K1520029 range2385718 1 3482 3610 BBa_K1520002 1 BBa_K1520002 Riboswitch MC31 2014-09-21T11:00:00Z 2015-05-08T01:10:48Z The aptamers are from results of paper, In vitro selection of specific aptamers against microcysitin-LR. The whole riboswitches are designed. It is a riboswitch designed by our team. We design a lot of riboswitch for microcystin, and then choose two excellent ones, MC7 and MC31, by modeling. They are riboswitches based on the stability of mRNA. The structure of them include a hammerhead ribozyme and a microcystin aptamer. Users can connect them down the promoter to use them. false false _1902_ 0 21318 9 Not in stock false We tested a lot of riboswitches by model and experiment to choose better one. false Xiaoman Xie BBa_K1520029 1 BBa_K1520029 Terminator 2014-09-21T11:00:00Z 2015-05-08T01:10:49Z It's designed. It's a terminator with two hairpin. false false _1902_ 0 21318 9 In stock false None false Peiqing Sun BBa_K1520504 1 BBa_K1520504 Rbs-luxI(scarless) 2014-09-21T11:00:00Z 2015-05-08T01:10:49Z It is an improvement of luxI(BBa_C0061) by adding a rbs. Rbs-luxI(scarless) It is an improvement of luxI(BBa_C0061) by adding a rbs. false false _1902_ 0 21318 9 Not in stock false None false yue wu annotation2384869 1 rbs range2384869 1 1 18 annotation2384870 1 luxI range2384870 1 19 661 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_K1520504_sequence 1 aaagaggagaaattaagcatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc BBa_R0062_sequence 1 acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa BBa_K1520507_sequence 1 atgaaaaagacagctatcgcgattgcagtggcactggctggtttcgctaccgtagcgcaggccgctccgaaagataacacctggtacactggtgctaaactgggctggtcccagtaccatgacactggtttcatcaacaacaatggcccgacccatgaaaaccaactgggcgctggtgcttttggtggttaccaggttaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggagtcgacatgcagttccatattgatgacatgacctgcggcggctgcgccagtacggtaaaaaagacgattctgactctcgatgctaatgcgacggtgagaactgacccggcgacgcgtctggttgacgttgaaacgtcgctatccgcggagcagattgccgccgccctgcaaaaggccggtttcccgccgcgcgagaggctcgaggattacaaggatgacgacgataagatctga BBa_K1520002_sequence 1 aaacaaacaaagctgtcaccggatgtgctttccggtctgatgagtccgtgtccgggagtgcgtgcacctgcacctgggggggtgttcgcttgggacgggacgaggacgaaacagcaaaaagaaaaataaaaa BBa_K1520501_sequence 1 aaagaggagaaattaagcatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac BBa_K1520003_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaattaagcatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1520029_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1520516_sequence 1 aaacaaacaaagctgtcaccggatgtgctttccggtctgatgagtccgtgtccgggagtgcgtgcacctgcacctgggggggtgttcgcttgggacgggacgaggacgaaacagcaaaaagaaaaataaaaatactagagaaagaggagaaattaagcatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaattaagcatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagatgaaaaagacagctatcgcgattgcagtggcactggctggtttcgctaccgtagcgcaggccgcttacccatacgacgtaccagattacgctgttgctccgaaagataacacctggtacactggtgctaaactgggctggtcccagtaccatgacactggtttcatcaacaacaatggcccgacccatgaaaaccaactgggcgctggtgcttttggtggttaccaggttaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggagtcgacatgcagttccatattgatgacatgacctgcggcggctgcgccagtacggtaaaaaagacgattctgactctcgatgctaatgcgacggtgagaactgacccggcgacgcgtctggttgacgttgaaacgtcgctatccgcggagcagattgccgccgccctgcaaaaggccggtttcccgccgcgcgagaggctcgaggattacaaggatgacgacgataagatctgatactagagaaagaggagaaattaagcatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z