##gff-version 3
##sequence-region BBa_K1520516 1 3610
BBa_K1520516	.	promoter	1	132	.	+	0	ID=BBa_K1520002;Name=BBa_K1520002
BBa_K1520516	.	terminator	3482	3610	.	+	0	ID=BBa_K1520029;Name=BBa_K1520029
BBa_K1520516	.	promoter	1934	1988	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K1520516	.	promoter	1975	1980	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1520516	.	engineered_region	1934	1988	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1520516	.	promoter	1953	1958	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1520516	.	start_codon	1986	1986	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1520516	.	non_covalent_binding_site	1934	1953	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1520516	.	terminator	810	938	.	+	0	ID=BBa_K1520029;Name=BBa_K1520029
BBa_K1520516	.	engineered_region	141	801	.	+	0	ID=BBa_K1520504;Name=BBa_K1520504
BBa_K1520516	.	ribosome_entry_site	141	158	.	+	0	ID=annotation2384869;Name=rbs;Parent=BBa_K1520504
BBa_K1520516	.	CDS	159	801	.	+	0	ID=annotation2384870;Name=luxI;Parent=BBa_K1520504
BBa_K1520516	.	engineered_region	2813	3473	.	+	0	ID=BBa_K1520504;Name=BBa_K1520504
BBa_K1520516	.	ribosome_entry_site	2813	2830	.	+	0	ID=annotation2384869;Name=rbs;Parent=BBa_K1520504
BBa_K1520516	.	CDS	2831	3473	.	+	0	ID=annotation2384870;Name=luxI;Parent=BBa_K1520504
BBa_K1520516	.	CDS	1995	2804	.	+	0	ID=BBa_K1520507;Name=BBa_K1520507
BBa_K1520516	.	CDS	2544	2735	.	+	0	ID=annotation2386995;Name=golB;Parent=BBa_K1520507
BBa_K1520516	.	sequence_feature	1995	2060	.	+	0	ID=annotation2386992;Name=singal peptide;Parent=BBa_K1520507
BBa_K1520516	.	null	2535	2543	.	+	0	ID=annotation2386994;Name=linker;Parent=BBa_K1520507
BBa_K1520516	.	tag	2736	2771	.	+	0	ID=annotation2386996;Name=flag;Parent=BBa_K1520507
BBa_K1520516	.	CDS	2061	2534	.	+	0	ID=annotation2386993;Name=OMPA;Parent=BBa_K1520507
BBa_K1520516	.	engineered_region	947	1925	.	+	0	ID=BBa_K1520003;Name=BBa_K1520003
BBa_K1520516	.	terminator	1797	1925	.	+	0	ID=BBa_K1520029;Name=BBa_K1520029;Parent=BBa_K1520003
BBa_K1520516	.	promoter	947	981	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K1520003
BBa_K1520516	.	engineered_region	990	1788	.	+	0	ID=BBa_K1520501;Name=BBa_K1520501;Parent=BBa_K1520003
BBa_K1520516	.	CDS	1008	1788	.	+	0	ID=annotation2384862;Name=luxR;Parent=BBa_K1520501
BBa_K1520516	.	ribosome_entry_site	990	1007	.	+	0	ID=annotation2384861;Name=rbs;Parent=BBa_K1520501
>BBa_K1520516
aaacaaacaaagctgtcaccggatgtgctttccggtctgatgagtccgtgtccgggagtgcgtgcacctgcacctgggg
gggtgttcgcttgggacgggacgaggacgaaacagcaaaaagaaaaataaaaatactagagaaagaggagaaattaagc
atgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatc
aagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgc
agaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatg
ctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttg
ctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaaca
cgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagtt
ccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatg
aacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgcta
gtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtt
gacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaattaagcatgaaaaacataaatgccga
cgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgact
aaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctag
ataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaa
ctccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaa
gcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttg
cacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctct
agttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgttta
gcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatt
taaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattga
ttgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgtt
atagtcgaataaatactagatgaaaaagacagctatcgcgattgcagtggcactggctggtttcgctaccgtagcgcag
gccgcttacccatacgacgtaccagattacgctgttgctccgaaagataacacctggtacactggtgctaaactgggct
ggtcccagtaccatgacactggtttcatcaacaacaatggcccgacccatgaaaaccaactgggcgctggtgcttttgg
tggttaccaggttaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgtt
gaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatct
acactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttc
tccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaac
atcggtgacgcacacaccatcggcactcgtccggacaacggagtcgacatgcagttccatattgatgacatgacctgcg
gcggctgcgccagtacggtaaaaaagacgattctgactctcgatgctaatgcgacggtgagaactgacccggcgacgcg
tctggttgacgttgaaacgtcgctatccgcggagcagattgccgccgccctgcaaaaggccggtttcccgccgcgcgag
aggctcgaggattacaaggatgacgacgataagatctgatactagagaaagaggagaaattaagcatgactataatgat
aaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaa
agacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatg
cttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttt
tcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaat
agctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaag
gtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtat
tggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaa
gcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctac
tagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaac
gctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata