##gff-version 3
##sequence-region BBa_K1520519 1 2540
BBa_K1520519	.	engineered_region	1	1601	.	+	0	ID=BBa_K1520518;Name=BBa_K1520518
BBa_K1520519	.	engineered_region	762	1464	.	+	0	ID=BBa_K1520505;Name=BBa_K1520505;Parent=BBa_K1520518
BBa_K1520519	.	CDS	780	1464	.	+	0	ID=annotation2384878;Name=tetR;Parent=BBa_K1520505
BBa_K1520519	.	ribosome_entry_site	762	779	.	+	0	ID=annotation2384877;Name=rbs;Parent=BBa_K1520505
BBa_K1520519	.	promoter	1	753	.	+	0	ID=BBa_K1520506;Name=BBa_K1520506;Parent=BBa_K1520518
BBa_K1520519	.	promoter	554	753	.	+	0	ID=annotation2385509;Name=PgolB;Parent=BBa_K1520506
BBa_K1520519	.	CDS	212	553	.	+	0	ID=annotation2385508;Name=golS;Parent=BBa_K1520506
BBa_K1520519	.	promoter	1	200	.	+	0	ID=annotation2385507;Name=PgolTS;Parent=BBa_K1520506
BBa_K1520519	.	terminator	1473	1601	.	+	0	ID=BBa_K1520029;Name=BBa_K1520029;Parent=BBa_K1520518
BBa_K1520519	.	engineered_region	1610	2540	.	+	0	ID=BBa_K1520014;Name=BBa_K1520014
BBa_K1520519	.	promoter	1610	1663	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K1520014
BBa_K1520519	.	promoter	1629	1634	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1520519	.	non_covalent_binding_site	1635	1653	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1520519	.	engineered_region	1610	1663	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1520519	.	non_covalent_binding_site	1610	1628	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1520519	.	promoter	1652	1657	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1520519	.	engineered_region	1672	2403	.	+	0	ID=BBa_K1520500;Name=BBa_K1520500;Parent=BBa_K1520014
BBa_K1520519	.	terminator	2412	2540	.	+	0	ID=BBa_K1520029;Name=BBa_K1520029;Parent=BBa_K1520014
>BBa_K1520519
acaacgaaggaaaggtcaagcgttcctgacgggtttttacggggcgtgggtcggcatcgtggcgtaaatgtctcgcatc
atcctcttttatgagccatctcacattctcgccgaaccgtgcagcctgaatacgcttgaccttcccacaatggcaagct
ttaggctttctgataccgaatagtcaggatggggaagtcgtcggaggagcgtcatgaacatcggtaaagcagctaaagc
atcgaaagtctcggccaaaatgattcgctactatgaacagattggtctgattcccgcggcaagtcggacggattccggc
tatcgggcctatacccaggctgatgttaatcaattgcattttatacgccgcgcgcgcgacctcggtttttcagttgctg
aaatcagcgacttactgaatctttggaataaccagtcgcggcaaagcgctgacgtcaaacgcctggcgcagacgcacat
tgatgaactggacagacgtatccagaacatgcagcacatggcgcaaaccctcaaagcgctgattcactgctgcgccggc
gacgcgctgccagattgccccattctgcatacgcttggacaacctgacgatagcgagccggaggcgcgtaccggagcgg
tattgcgacgtcctcgtcgccacggactggcaaagcgtctgtaagtcctgagattacgcttgaccttccaacactggca
aggtccagactggcaacagttcccacacaaaaggagttcacttactagagaaagaggagaaattaagcatgtccagatt
agataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactc
gcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagcca
ttgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgc
taaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacag
tatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcg
ctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctac
tactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattc
ggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacg
ctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactga
gcactactagagaaagaggagaaattaagcatggtgagcaagggcgaggaggataacatggccatcatcaaggagttca
tgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgccccta
cgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcag
ttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggct
tcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcga
gttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgg
gaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacg
gcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaa
catcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactcc
accggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagac
tgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctt
tctgcgtttata