PgolTS-golS-PgolB, tetR, PtetO, rfp, Plac, Ter
PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter-Plac-tetR-Ter It is part of our test of suicide circuit by gold ions.
Ptet-rbs-rfp-Ter. It produces rfp when there is less tetR.
Plac-rbs-tetR-Ter. It produces tetR when there is enough IPTG or less lacI.
PgolTS-golS-PgolB-tetR-Ter If there exist gold ions, tetR will be produced.
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
Sequence for pTet inverting regulator driven by the TetR protein.
BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
It is an improvement of tetR(BBa_C0040) by adding a rbs.
Rbs-tetR(scarless) It is an improvement of tetR(BBa_C0040) by adding a rbs.
It is a gold ion detector. Gold ions will cause the CDS after PgolB to be expressed. PgolTS works like a constant promoter, producing gols which is inhibitor of Pgols. Once combining gold ions, gols will stop inhibitor PgolB. So, when there are gold ions, the CDS after this part will be expressed.
Conversion of the iGEM parts registry to SBOL2.1