BBa_K1520520
type: ComponentDefinition
persistent identity: BBa K1520520
display id: BBa_K1520520
version: 1
was generated by: iGEM to SBOL conversion
description: PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter-Plac-tetR-Ter
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:49Z
mutable provenance: PgolTS-golS-PgolB, tetR, PtetO, rfp, Plac, Ter
owned by: james
owned by: myers
mutable description: PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter-Plac-tetR-Ter It is part of our test of suicide circuit by gold ions.
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: yue wu
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1520015
component: BBa_K1520014
component: BBa_K1520518
sequence annotation: BBa_K1520518
sequence annotation: BBa_K1520014
sequence annotation: BBa_K1520015
sequence: https://synbiohub.org/public/igem/BBa_K1520520_sequence/1

BBa_K1520015
type: Component
persistent identity: component2386991
display id: component2386991
version: 1
top level: BBa_K1520520
definition: BBa_K1520015
access: public

BBa_K1520014
type: Component
persistent identity: component2386979
display id: component2386979
version: 1
top level: BBa_K1520520
definition: BBa_K1520014
access: public

BBa_K1520518
type: Component
persistent identity: component2386971
display id: component2386971
version: 1
top level: BBa_K1520520
definition: BBa_K1520518
access: public

BBa_K1520518
type: SequenceAnnotation
persistent identity: annotation2386971
display id: annotation2386971
version: 1
top level: BBa_K1520520
location: https://synbiohub.org/public/igem/BBa_K1520520/annotation2386971/range2386971/1
component: BBa_K1520518

https://synbiohub.org/public/igem/BBa_K1520520/annotation2386971/range2386971/1
type: Range
persistent identity: range2386971
display id: range2386971
version: 1
top level: BBa_K1520520
direction: direction/reverse
start: 1
end: 1601
orientation: inline

BBa_K1520014
type: SequenceAnnotation
persistent identity: annotation2386979
display id: annotation2386979
version: 1
top level: BBa_K1520520
location: https://synbiohub.org/public/igem/BBa_K1520520/annotation2386979/range2386979/1
component: BBa_K1520014

https://synbiohub.org/public/igem/BBa_K1520520/annotation2386979/range2386979/1
type: Range
persistent identity: range2386979
display id: range2386979
version: 1
top level: BBa_K1520520
direction: direction/reverse
start: 1610
end: 2540
orientation: inline

BBa_K1520015
type: SequenceAnnotation
persistent identity: annotation2386991
display id: annotation2386991
version: 1
top level: BBa_K1520520
location: https://synbiohub.org/public/igem/BBa_K1520520/annotation2386991/range2386991/1
component: BBa_K1520015

https://synbiohub.org/public/igem/BBa_K1520520/annotation2386991/range2386991/1
type: Range
persistent identity: range2386991
display id: range2386991
version: 1
top level: BBa_K1520520
direction: direction/reverse
start: 2549
end: 3596
orientation: inline

BBa_K1520014
type: ComponentDefinition
persistent identity: BBa K1520014
display id: BBa_K1520014
version: 1
was generated by: iGEM to SBOL conversion
description: Ptet-rbs-rfp-Ter
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
mutable provenance: Ptet, rfp, Ter
owned by: james
owned by: myers
mutable description: Ptet-rbs-rfp-Ter. It produces rfp when there is less tetR.
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: yue wu
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1520029
component: BBa_K1520500
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_K1520500
sequence annotation: BBa_K1520029
sequence: https://synbiohub.org/public/igem/BBa_K1520014_sequence/1

BBa_K1520029
type: Component
persistent identity: component2384998
display id: component2384998
version: 1
top level: BBa_K1520014
definition: BBa_K1520029
access: public

BBa_K1520500
type: Component
persistent identity: component2384997
display id: component2384997
version: 1
top level: BBa_K1520014
definition: BBa_K1520500
access: public

BBa_R0040
type: Component
persistent identity: component2384992
display id: component2384992
version: 1
top level: BBa_K1520014
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2384992
display id: annotation2384992
version: 1
top level: BBa_K1520014
location: https://synbiohub.org/public/igem/BBa_K1520014/annotation2384992/range2384992/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1520014/annotation2384992/range2384992/1
type: Range
persistent identity: range2384992
display id: range2384992
version: 1
top level: BBa_K1520014
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_K1520500
type: SequenceAnnotation
persistent identity: annotation2384997
display id: annotation2384997
version: 1
top level: BBa_K1520014
location: https://synbiohub.org/public/igem/BBa_K1520014/annotation2384997/range2384997/1
component: BBa_K1520500

https://synbiohub.org/public/igem/BBa_K1520014/annotation2384997/range2384997/1
type: Range
persistent identity: range2384997
display id: range2384997
version: 1
top level: BBa_K1520014
direction: direction/reverse
start: 63
end: 794
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384998
display id: annotation2384998
version: 1
top level: BBa_K1520014
location: https://synbiohub.org/public/igem/BBa_K1520014/annotation2384998/range2384998/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520014/annotation2384998/range2384998/1
type: Range
persistent identity: range2384998
display id: range2384998
version: 1
top level: BBa_K1520014
direction: direction/reverse
start: 803
end: 931
orientation: inline

BBa_K1520015
type: ComponentDefinition
persistent identity: BBa K1520015
display id: BBa_K1520015
version: 1
was generated by: iGEM to SBOL conversion
description: Plac-rbs-tetR-Ter
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
mutable provenance: Plac, tetR, Ter
owned by: james
owned by: myers
mutable description: Plac-rbs-tetR-Ter. It produces tetR when there is enough IPTG or less lacI.
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: yue wu
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520029
component: BBa_K1520505
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_K1520505
sequence annotation: BBa_K1520029
sequence: https://synbiohub.org/public/igem/BBa_K1520015_sequence/1

BBa_K1520029
type: Component
persistent identity: component2385009
display id: component2385009
version: 1
top level: BBa_K1520015
definition: BBa_K1520029
access: public

BBa_K1520505
type: Component
persistent identity: component2385008
display id: component2385008
version: 1
top level: BBa_K1520015
definition: BBa_K1520505
access: public

BBa_R0010
type: Component
persistent identity: component2384999
display id: component2384999
version: 1
top level: BBa_K1520015
definition: LacI
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384999
display id: annotation2384999
version: 1
top level: BBa_K1520015
location: https://synbiohub.org/public/igem/BBa_K1520015/annotation2384999/range2384999/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1520015/annotation2384999/range2384999/1
type: Range
persistent identity: range2384999
display id: range2384999
version: 1
top level: BBa_K1520015
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_K1520505
type: SequenceAnnotation
persistent identity: annotation2385008
display id: annotation2385008
version: 1
top level: BBa_K1520015
location: https://synbiohub.org/public/igem/BBa_K1520015/annotation2385008/range2385008/1
component: BBa_K1520505

https://synbiohub.org/public/igem/BBa_K1520015/annotation2385008/range2385008/1
type: Range
persistent identity: range2385008
display id: range2385008
version: 1
top level: BBa_K1520015
direction: direction/reverse
start: 209
end: 911
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385009
display id: annotation2385009
version: 1
top level: BBa_K1520015
location: https://synbiohub.org/public/igem/BBa_K1520015/annotation2385009/range2385009/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520015/annotation2385009/range2385009/1
type: Range
persistent identity: range2385009
display id: range2385009
version: 1
top level: BBa_K1520015
direction: direction/reverse
start: 920
end: 1048
orientation: inline

BBa_K1520518
type: ComponentDefinition
persistent identity: BBa K1520518
display id: BBa_K1520518
version: 1
was generated by: iGEM to SBOL conversion
description: PgolTS-golS-PgolB-tetR-Ter
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:49Z
mutable provenance: PgolTS-golS-PgolB, tetR, Ter
owned by: james
owned by: myers
mutable description: PgolTS-golS-PgolB-tetR-Ter If there exist gold ions, tetR will be produced.
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: yue wu
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1520506
component: BBa_K1520029
component: BBa_K1520505
sequence annotation: BBa_K1520506
sequence annotation: BBa_K1520505
sequence annotation: BBa_K1520029
sequence: https://synbiohub.org/public/igem/BBa_K1520518_sequence/1

BBa_K1520506
type: Component
persistent identity: component2385739
display id: component2385739
version: 1
top level: BBa_K1520518
definition: BBa_K1520506
access: public

BBa_K1520029
type: Component
persistent identity: component2385743
display id: component2385743
version: 1
top level: BBa_K1520518
definition: BBa_K1520029
access: public

BBa_K1520505
type: Component
persistent identity: component2385742
display id: component2385742
version: 1
top level: BBa_K1520518
definition: BBa_K1520505
access: public

BBa_K1520506
type: SequenceAnnotation
persistent identity: annotation2385739
display id: annotation2385739
version: 1
top level: BBa_K1520518
location: https://synbiohub.org/public/igem/BBa_K1520518/annotation2385739/range2385739/1
component: BBa_K1520506

https://synbiohub.org/public/igem/BBa_K1520518/annotation2385739/range2385739/1
type: Range
persistent identity: range2385739
display id: range2385739
version: 1
top level: BBa_K1520518
direction: direction/reverse
start: 1
end: 753
orientation: inline

BBa_K1520505
type: SequenceAnnotation
persistent identity: annotation2385742
display id: annotation2385742
version: 1
top level: BBa_K1520518
location: https://synbiohub.org/public/igem/BBa_K1520518/annotation2385742/range2385742/1
component: BBa_K1520505

https://synbiohub.org/public/igem/BBa_K1520518/annotation2385742/range2385742/1
type: Range
persistent identity: range2385742
display id: range2385742
version: 1
top level: BBa_K1520518
direction: direction/reverse
start: 762
end: 1464
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385743
display id: annotation2385743
version: 1
top level: BBa_K1520518
location: https://synbiohub.org/public/igem/BBa_K1520518/annotation2385743/range2385743/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520518/annotation2385743/range2385743/1
type: Range
persistent identity: range2385743
display id: range2385743
version: 1
top level: BBa_K1520518
direction: direction/reverse
start: 1473
end: 1601
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_K1520029
type: ComponentDefinition
persistent identity: BBa K1520029
display id: BBa_K1520029
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
mutable provenance: It's designed.
owned by: james
owned by: myers
mutable description: It's a terminator with two hairpin.
discontinued: false
dominant: false
experience: experience/None
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: None
star: false
creator: Peiqing Sun
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_K1520029_sequence/1

BBa_K1520500
type: ComponentDefinition
persistent identity: BBa K1520500
display id: BBa_K1520500
version: 1
was generated by: iGEM to SBOL conversion
description: red fluorescent protein
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
mutable provenance: NONE
owned by: james
owned by: myers
mutable description: red fluorescent protein
discontinued: false
dominant: false
experience: experience/None
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: NONE
star: false
creator: Peiqing Sun
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence: https://synbiohub.org/public/igem/BBa_K1520500_sequence/1

BBa_K1520505
type: ComponentDefinition
persistent identity: BBa K1520505
display id: BBa_K1520505
version: 1
was generated by: iGEM to SBOL conversion
description: Rbs-tetR(scarless)
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
mutable provenance: It is an improvement of tetR(BBa_C0040) by adding a rbs.
owned by: james
owned by: myers
mutable description: Rbs-tetR(scarless) It is an improvement of tetR(BBa_C0040) by adding a rbs.
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: yue wu
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: rbs
sequence annotation: tetR
sequence: https://synbiohub.org/public/igem/BBa_K1520505_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2384877
display id: annotation2384877
version: 1
top level: BBa_K1520505
location: https://synbiohub.org/public/igem/BBa_K1520505/annotation2384877/range2384877/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1520505/annotation2384877/range2384877/1
type: Range
persistent identity: range2384877
display id: range2384877
version: 1
top level: BBa_K1520505
direction: direction/forward
start: 1
end: 18
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation2384878
display id: annotation2384878
version: 1
top level: BBa_K1520505
location: https://synbiohub.org/public/igem/BBa_K1520505/annotation2384878/range2384878/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1520505/annotation2384878/range2384878/1
type: Range
persistent identity: range2384878
display id: range2384878
version: 1
top level: BBa_K1520505
direction: direction/forward
start: 19
end: 703
orientation: inline

BBa_K1520506
type: ComponentDefinition
persistent identity: BBa K1520506
display id: BBa_K1520506
version: 1
was generated by: iGEM to SBOL conversion
description: PgolTS-golS-PgolB
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:49Z
mutable provenance: PgolTS, golS, PgolB
owned by: james
owned by: myers
mutable description: It is a gold ion detector. Gold ions will cause the CDS after PgolB to be expressed. PgolTS works like a constant promoter, producing gols which is inhibitor of Pgols. Once combining gold ions, gols will stop inhibitor PgolB. So, when there are gold ions, the CDS after this part will be expressed.
discontinued: false
dominant: false
experience: experience/None
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Wei wei
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: PgolTS
sequence annotation: golS
sequence annotation: PgolB
sequence: https://synbiohub.org/public/igem/BBa_K1520506_sequence/1

PgolTS
type: SequenceAnnotation
persistent identity: annotation2385507
display id: annotation2385507
version: 1
top level: BBa_K1520506
location: https://synbiohub.org/public/igem/BBa_K1520506/annotation2385507/range2385507/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K1520506/annotation2385507/range2385507/1
type: Range
persistent identity: range2385507
display id: range2385507
version: 1
top level: BBa_K1520506
direction: direction/forward
start: 1
end: 200
orientation: inline

golS
type: SequenceAnnotation
persistent identity: annotation2385508
display id: annotation2385508
version: 1
top level: BBa_K1520506
location: https://synbiohub.org/public/igem/BBa_K1520506/annotation2385508/range2385508/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1520506/annotation2385508/range2385508/1
type: Range
persistent identity: range2385508
display id: range2385508
version: 1
top level: BBa_K1520506
direction: direction/forward
start: 212
end: 553
orientation: inline

PgolB
type: SequenceAnnotation
persistent identity: annotation2385509
display id: annotation2385509
version: 1
top level: BBa_K1520506
location: https://synbiohub.org/public/igem/BBa_K1520506/annotation2385509/range2385509/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K1520506/annotation2385509/range2385509/1
type: Range
persistent identity: range2385509
display id: range2385509
version: 1
top level: BBa_K1520506
direction: direction/forward
start: 554
end: 753
orientation: inline

https://synbiohub.org/public/igem/BBa_K1520520_sequence/1
type: Sequence
persistent identity: BBa K1520520 sequence
display id: BBa_K1520520_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acaacgaaggaaaggtcaagcgttcctgacgggtttttacggggcgtgggtcggcatcgtggcgtaaatgtctcgcatcatcctcttttatgagccatctcacattctcgccgaaccgtgcagcctgaatacgcttgaccttcccacaatggcaagctttaggctttctgataccgaatagtcaggatggggaagtcgtcggaggagcgtcatgaacatcggtaaagcagctaaagcatcgaaagtctcggccaaaatgattcgctactatgaacagattggtctgattcccgcggcaagtcggacggattccggctatcgggcctatacccaggctgatgttaatcaattgcattttatacgccgcgcgcgcgacctcggtttttcagttgctgaaatcagcgacttactgaatctttggaataaccagtcgcggcaaagcgctgacgtcaaacgcctggcgcagacgcacattgatgaactggacagacgtatccagaacatgcagcacatggcgcaaaccctcaaagcgctgattcactgctgcgccggcgacgcgctgccagattgccccattctgcatacgcttggacaacctgacgatagcgagccggaggcgcgtaccggagcggtattgcgacgtcctcgtcgccacggactggcaaagcgtctgtaagtcctgagattacgcttgaccttccaacactggcaaggtccagactggcaacagttcccacacaaaaggagttcacttactagagaaagaggagaaattaagcatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaattaagcatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaactt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1520014_sequence/1
type: Sequence
persistent identity: BBa K1520014 sequence
display id: BBa_K1520014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaattaagcatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1520015_sequence/1
type: Sequence
persistent identity: BBa K1520015 sequence
display id: BBa_K1520015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaattaagcatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1520518_sequence/1
type: Sequence
persistent identity: BBa K1520518 sequence
display id: BBa_K1520518_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acaacgaaggaaaggtcaagcgttcctgacgggtttttacggggcgtgggtcggcatcgtggcgtaaatgtctcgcatcatcctcttttatgagccatctcacattctcgccgaaccgtgcagcctgaatacgcttgaccttcccacaatggcaagctttaggctttctgataccgaatagtcaggatggggaagtcgtcggaggagcgtcatgaacatcggtaaagcagctaaagcatcgaaagtctcggccaaaatgattcgctactatgaacagattggtctgattcccgcggcaagtcggacggattccggctatcgggcctatacccaggctgatgttaatcaattgcattttatacgccgcgcgcgcgacctcggtttttcagttgctgaaatcagcgacttactgaatctttggaataaccagtcgcggcaaagcgctgacgtcaaacgcctggcgcagacgcacattgatgaactggacagacgtatccagaacatgcagcacatggcgcaaaccctcaaagcgctgattcactgctgcgccggcgacgcgctgccagattgccccattctgcatacgcttggacaacctgacgatagcgagccggaggcgcgtaccggagcggtattgcgacgtcctcgtcgccacggactggcaaagcgtctgtaagtcctgagattacgcttgaccttccaacactggcaaggtccagactggcaacagttcccacacaaaaggagttcacttactagagaaagaggagaaattaagcatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1520029_sequence/1
type: Sequence
persistent identity: BBa K1520029 sequence
display id: BBa_K1520029_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1520500_sequence/1
type: Sequence
persistent identity: BBa K1520500 sequence
display id: BBa_K1520500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaattaagcatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1520505_sequence/1
type: Sequence
persistent identity: BBa K1520505 sequence
display id: BBa_K1520505_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaattaagcatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1520506_sequence/1
type: Sequence
persistent identity: BBa K1520506 sequence
display id: BBa_K1520506_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acaacgaaggaaaggtcaagcgttcctgacgggtttttacggggcgtgggtcggcatcgtggcgtaaatgtctcgcatcatcctcttttatgagccatctcacattctcgccgaaccgtgcagcctgaatacgcttgaccttcccacaatggcaagctttaggctttctgataccgaatagtcaggatggggaagtcgtcggaggagcgtcatgaacatcggtaaagcagctaaagcatcgaaagtctcggccaaaatgattcgctactatgaacagattggtctgattcccgcggcaagtcggacggattccggctatcgggcctatacccaggctgatgttaatcaattgcattttatacgccgcgcgcgcgacctcggtttttcagttgctgaaatcagcgacttactgaatctttggaataaccagtcgcggcaaagcgctgacgtcaaacgcctggcgcagacgcacattgatgaactggacagacgtatccagaacatgcagcacatggcgcaaaccctcaaagcgctgattcactgctgcgccggcgacgcgctgccagattgccccattctgcatacgcttggacaacctgacgatagcgagccggaggcgcgtaccggagcggtattgcgacgtcctcgtcgccacggactggcaaagcgtctgtaagtcctgagattacgcttgaccttccaacactggcaaggtccagactggcaacagttcccacacaaaaggagttcact
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z