##gff-version 3
##sequence-region BBa_K1521002 1 4719
BBa_K1521002	.	engineered_region	3413	4719	.	+	0	ID=BBa_P0312;Name=BBa_P0312
BBa_K1521002	.	terminator	4679	4719	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0312
BBa_K1521002	.	stop_codon	4712	4712	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1521002	.	polyA_site	4706	4719	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1521002	.	stem_loop	4686	4705	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1521002	.	engineered_region	4679	4719	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1521002	.	terminator	4591	4670	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0312
BBa_K1521002	.	engineered_region	4591	4670	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1521002	.	stem_loop	4602	4645	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1521002	.	CDS	3430	4557	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_P0312
BBa_K1521002	.	engineered_tag	4558	4582	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K1521002	.	engineered_region	3430	4557	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K1521002	.	tag	4519	4557	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K1521002	.	CDS	3430	4557	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K1521002	.	ribosome_entry_site	3413	3423	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_P0312
BBa_K1521002	.	ribosome_entry_site	3413	3423	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K1521002	.	engineered_region	3413	3423	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K1521002	.	ribosome_entry_site	3419	3422	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K1521002	.	promoter	1129	1365	.	+	0	ID=BBa_K823003;Name=BBa_K823003
BBa_K1521002	.	promoter	1129	1365	.	+	0	ID=annotation2190180;Name=promoter;Parent=BBa_K823003
BBa_K1521002	.	promoter	1	237	.	+	0	ID=BBa_K823003;Name=BBa_K823003
BBa_K1521002	.	promoter	1	237	.	+	0	ID=annotation2190180;Name=promoter;Parent=BBa_K823003
BBa_K1521002	.	engineered_region	1374	2273	.	+	0	ID=BBa_K081001;Name=BBa_K081001
BBa_K1521002	.	terminator	2070	2108	.	+	0	ID=BBa_B1006;Name=BBa_B1006;Parent=BBa_K081001
BBa_K1521002	.	polyA_site	2070	2078	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K1521002	.	polyA_site	2101	2108	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K1521002	.	stem_loop	2079	2100	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K1521002	.	engineered_region	2070	2108	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K1521002	.	CDS	1395	2061	.	+	0	ID=BBa_C0078;Name=BBa_C0078;Parent=BBa_K081001
BBa_K1521002	.	tag	1998	2030	.	+	0	ID=annotation306608;Name=LVA;Parent=BBa_C0078
BBa_K1521002	.	engineered_tag	2037	2061	.	+	0	ID=annotation2214003;Name=Help:Barcodes;Parent=BBa_C0078
BBa_K1521002	.	CDS	1395	1997	.	+	0	ID=annotation305970;Name=LasI;Parent=BBa_C0078
BBa_K1521002	.	promoter	2117	2273	.	+	0	ID=BBa_R0079;Name=BBa_R0079;Parent=BBa_K081001
BBa_K1521002	.	non_covalent_binding_site	2222	2241	.	+	0	ID=annotation300992;Name=OP1;Parent=BBa_R0079
BBa_K1521002	.	non_covalent_binding_site	2162	2179	.	+	0	ID=annotation300985;Name=OP2;Parent=BBa_R0079
BBa_K1521002	.	promoter	2233	2238	.	+	0	ID=annotation318495;Name=-35;Parent=BBa_R0079
BBa_K1521002	.	promoter	2256	2261	.	+	0	ID=annotation318496;Name=-10;Parent=BBa_R0079
BBa_K1521002	.	ribosome_entry_site	1374	1388	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081001
BBa_K1521002	.	ribosome_entry_site	1381	1385	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1521002	.	engineered_region	1374	1388	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1521002	.	ribosome_entry_site	1374	1388	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1521002	.	engineered_region	246	983	.	+	0	ID=BBa_K1521001;Name=BBa_K1521001
BBa_K1521002	.	ribosome_entry_site	246	257	.	+	0	ID=annotation2391929;Name=rbs;Parent=BBa_K1521001
BBa_K1521002	.	CDS	264	983	.	+	0	ID=annotation2391930;Name=cds;Parent=BBa_K1521001
BBa_K1521002	.	engineered_region	2282	3159	.	+	0	ID=BBa_E0840;Name=BBa_E0840
BBa_K1521002	.	ribosome_entry_site	2282	2296	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K1521002	.	ribosome_entry_site	2289	2293	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1521002	.	engineered_region	2282	2296	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1521002	.	ribosome_entry_site	2282	2296	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1521002	.	terminator	3119	3159	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K1521002	.	stop_codon	3152	3152	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1521002	.	polyA_site	3146	3159	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1521002	.	stem_loop	3126	3145	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1521002	.	engineered_region	3119	3159	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1521002	.	CDS	2303	3022	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K1521002	.	CDS	2303	3022	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1521002	.	terminator	3031	3110	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K1521002	.	engineered_region	3031	3110	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1521002	.	stem_loop	3042	3085	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1521002	.	promoter	3168	3404	.	+	0	ID=BBa_K823003;Name=BBa_K823003
BBa_K1521002	.	promoter	3168	3404	.	+	0	ID=annotation2190180;Name=promoter;Parent=BBa_K823003
BBa_K1521002	.	terminator	992	1120	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1521002	.	terminator	992	1071	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1521002	.	engineered_region	992	1071	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1521002	.	stem_loop	1003	1046	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1521002	.	terminator	1080	1120	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1521002	.	stop_codon	1113	1113	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1521002	.	polyA_site	1107	1120	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1521002	.	stem_loop	1087	1106	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1521002	.	engineered_region	1080	1120	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K1521002
ggagttctgagaattggtatgccttataagtccaattaacagttgaaaacctgcataggagagctatgcgggtttttta
ttttacataatgatacataatttaccgaaacttgcggaacataattgaggaatcatagaattttgtcaaaataatttta
ttgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgtagt
tactagagaaaggtggtgaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattgga
gtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagc
caggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgc
gggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaa
gcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggc
gaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccga
ccctgtggatgctcaaagactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggt
tctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaac
tgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggcca
ttatggccgttaatttgggtcttattactctctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagagggagttctgagaattggtatgccttataagtccaattaacagttgaaaacctgcata
ggagagctatgcgggttttttattttacataatgatacataatttaccgaaacttgcggaacataattgaggaatcata
gaattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggct
cgtgttgtacaataaatgtagttactagagattaaagaggagaaatactagatgatcgttcagatcggtcgtcgtgaag
agttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttc
cgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaa
gctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgc
acggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccct
gggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggtt
accgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaa
tcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggt
tgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgt
agatctctactagagaaaaaaaaaccccgcccctgacagggcggggtttttttttactagaggcccctcgctgagcgcg
tcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaa
atcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctactagagattaaagagg
agaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaa
tgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcact
actggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccag
atcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaaga
tgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtatt
gattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcag
acaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagacca
ttatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctt
tcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatgg
atgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatat
actagagggagttctgagaattggtatgccttataagtccaattaacagttgaaaacctgcataggagagctatgcggg
ttttttattttacataatgatacataatttaccgaaacttgcggaacataattgaggaatcatagaattttgtcaaaat
aattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataa
atgtagttactagagtcacacaggactactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgc
cggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaa
gcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcg
ttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgc
cagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgc
gtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccgg
cgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgt
ggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgt
ctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgt
ccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggc
gctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgatacc
gaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggacc
gcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccac
cctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccga
ctggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
tactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtg
aacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata