##gff-version 3
##sequence-region BBa_K1523101 1 3726
BBa_K1523101	.	promoter	1	100	.	+	0	ID=BBa_K216005;Name=BBa_K216005
BBa_K1523101	.	non_covalent_binding_site	89	94	.	+	0	ID=annotation2027075;Name=promoter -10 region;Parent=BBa_K216005
BBa_K1523101	.	non_covalent_binding_site	50	65	.	+	0	ID=annotation2027072;Name=NarL-binding region;Parent=BBa_K216005
BBa_K1523101	.	non_covalent_binding_site	58	80	.	+	0	ID=annotation2027073;Name=NsrR-binding region;Parent=BBa_K216005
BBa_K1523101	.	non_covalent_binding_site	66	71	.	+	0	ID=annotation2027074;Name=promoter -35 region;Parent=BBa_K216005
BBa_K1523101	.	CDS	130	3726	.	+	0	ID=BBa_K1172303;Name=BBa_K1172303
BBa_K1523101	.	CDS	130	1275	.	+	0	ID=annotation2370310;Name=ribD;Parent=BBa_K1172303
BBa_K1523101	.	start_codon	3250	3252	.	+	0	ID=annotation2370720;Name=atg;Parent=BBa_K1172303
BBa_K1523101	.	start_codon	2006	2008	.	+	0	ID=annotation2370719;Name=atg;Parent=BBa_K1172303
BBa_K1523101	.	CDS	3250	3726	.	+	0	ID=annotation2370313;Name=ribE;Parent=BBa_K1172303
BBa_K1523101	.	CDS	1283	1939	.	+	0	ID=annotation2370311;Name=SO_3468;Parent=BBa_K1172303
BBa_K1523101	.	start_codon	130	132	.	+	0	ID=annotation2370717;Name=atg;Parent=BBa_K1172303
BBa_K1523101	.	point_mutation	1773	1778	.	+	0	ID=annotation2370715;Name=Pst1 C->T;Parent=BBa_K1172303
BBa_K1523101	.	stop_codon	1273	1275	.	+	0	ID=annotation2370721;Name=tga;Parent=BBa_K1172303
BBa_K1523101	.	stop_codon	1937	1939	.	+	0	ID=annotation2370722;Name=tag;Parent=BBa_K1172303
BBa_K1523101	.	point_mutation	1969	1974	.	+	0	ID=annotation2370716;Name=EcoR1 C->T;Parent=BBa_K1172303
BBa_K1523101	.	CDS	2006	3109	.	+	0	ID=annotation2370312;Name=ribBA;Parent=BBa_K1172303
BBa_K1523101	.	start_codon	1283	1285	.	+	0	ID=annotation2370718;Name=atg;Parent=BBa_K1172303
BBa_K1523101	.	stop_codon	3724	3726	.	+	0	ID=annotation2370724;Name=taa;Parent=BBa_K1172303
BBa_K1523101	.	point_mutation	363	368	.	+	0	ID=annotation2370714;Name=Pst1 C->T;Parent=BBa_K1172303
BBa_K1523101	.	stop_codon	3107	3109	.	+	0	ID=annotation2370723;Name=taa;Parent=BBa_K1172303
BBa_K1523101	.	ribosome_entry_site	109	123	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1523101	.	ribosome_entry_site	116	120	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1523101	.	engineered_region	109	123	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1523101	.	ribosome_entry_site	109	123	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
>BBa_K1523101
ttcccatctataatcctccctgattcttcgctgatatggtgctaaaaagtaaccaataaatggtatttaaaatgcaaat
tatcaggcgtaccctgaaacgtactagagattaaagaggagaaatactagatgaattggtcagtactcgataaccaaat
gatgagccgagctattcaattggctcgcaaaggattttataccactcgccccaatcccagtgtgggctgcgttatcgtt
aacgataaccagattgtcggtgaaggttatcatcaaaaagccggagagccccacgctgaagtgcatgcgctgcgcatgg
ctggcgagcttgcccgtggcgcgactgcttatgtcaccttagaaccctgtagccattatggtcgtacgccgccctgcgc
cttggcgctgatcaatattggtgtaaagcgggtggtggtggcggttgaagatcctaatccgcaggtcggcgggcgcggt
attcaaatgctgcgcgatgcgggtattcaagtggatgtgggcttgcatcgtgatgaggcctatgccttaaatcttggtt
ttatgaagcgcatggagtcgggtctaccttgggtgacggtaaagcttgccgcgagtcttgatggtaaaactgcgttatc
aaacggggtgtctaaatggatcactggccctgaagctcgccgcgatgtgcagcgcttaagattacgatcctgcgcgctg
atcacggggattgaaacggtactggccgatgatccatcgctcaatgtgcgctactcagagcttggcagtttaagagcgc
aattgagtgagtcgcaggttttacagccgctgcgagtggttttggacagtcgcgcccgcatgcctatcacggcagcatt
gtttgcgattgaatcacccattttattagtctcgacagtgagctattcgtcagactttatggtgcaattacccgcccat
gtgacttgcctccaattaccagccattgagggtcgaatctccttgtctgcactattaagctatttaggcaaaagctgta
atcaggtgcttatcgaagcgggcgcgactttagctggcgcctttatcggctcaggattagccgatgaattagtgcttta
tcaagcgatgaaaatccttggcggccaaggacgtaatctacttgaattacccgattatcaaatgatggccgatattccg
accctcaaattggtcgacgagcgtaaagtgggcgcggatacgcgcttcaccttgaggctcgagtccaatccatctttag
cgaataagtgagttaaccatgtttactgggattattgaggccgttggtacactgcgaaagcttgagcgtaaaggcgatg
atattcgcttaacggtcgccagtggcaaactggatttaaccgatgtgcgtttaggcgacagtatcgccaccaacggtgt
atgtttgaccgtggttcaacagttagccgatggctatgtggctgatgtgtcagctgaaaccgtcagccttacaggtttt
gcaaactataaagtgggtactaaggtcaatcttgaaaaggccgtcaccccaactactcgtttaggcggccatatggtga
gtggtcatgtggatgggattgctactgttgagcagcgtcttgctcgtggtcaagcaatcgaattttggttagcggctcc
cacggagcttgctcgctatattgcccataaaggttctatcacgattgatggcgtgagcctgacagtaaacgaagtcgat
ggacaccgttttcgattaacgattgtgccccatactgtaggtgaaaccacgttggtggatttaaaagcaggcgataagg
tcaatattgaagtggacttaattgcccgttatttagagcgtttaatgcgttttgacagcaaagaaacccaaggcggagg
ggtgaccatggaaatgttagcccgtgctggctttgtgcgttagtacactggcactcagttcacttaggtatagaattta
taacaacagtcacattataaaggtcctacaatggcgctgcacagtatagaagagatcatcgaggatattcgtcaaggca
aaatggttattttgatggatgacgaggacagagaaaacgaaggcgatctgattttggcggccgaaatggtgacgccaga
agcgattaactttatggcgaaatacggccgcgggttaatctgccaaacgatgaccaaggcccgttgccagcagttaaat
ctgcccttgatggtaaccaataacaatgcgcagttctcgaccaactttacggtatcgattgaagctgccgaaggcgtaa
ccacagggatttcggcccacgatcgcgctgtaaccgttaaagcggccgtagcaaaagatgctaaagcgtcggacttagt
tcagcctgggcatatcttcccgttaatggcgcaggatggtggcgtattaacccgcgcaggtcacactgaagcgggctgt
gatttagcccgtttagcagggcttgaaccatcgggggtgattgttgaaattttgaacgaagacggcactatggcccgtc
gcccagatttagagattttctctgaaaaacatggcattaaaatcggcactattgcggccctgattgaataccgtaatac
caaagaaacgaccgttgtgcgtgaagccaaatgcaaactgccaacccgtttcggtgagttcgatatggtgacctttagg
gatactatcgacaatcaattgcactttgccttagtgaaaggcgaggtgaagcctgactgtttagtacgggtacatttac
agaatacctttaacgatctacttcactctgagcgtgatcagcaacgtagctggccgctcgaaaaagcgatggcgcggat
ttctgccgaaggtggtgtattagtactgcttggcaatcaggaacattccagtgaaatcctcgccaaagtcaaagccttt
gaagcggaagatcaaggtcagaccccagtatcggctaaatggcaaggcacctcacgccgtgtaggtgtgggttcgcaaa
tcctcgccagtttaggcgtgacaaaaatgcgactcttaagctcgcccaaacgttatcactcactttcaggctttggcct
tgaagtgaccgaatgcgtagctgagtaacaggttaagcttgtaataggttgcactaaggtaaaattaaccatttctatc
atttgcataaggccgcgggcaattgactgtggtatcatgtcgccacttttcgcccagagctgggtgctttagctaaatt
aggtaagaaaatgaacgtagttcaaggtaatatcgaagcgaagaatgccaaagttgcgattgtaatttcgcgttttaac
agctttttagtcgaaagcctgcttgaaggtgcacttgacacgctgaaacgttttggccaagtcagtgatgaaaacatca
ctgttgtccgtgtacctggtgcggttgagttaccgctggctgcacgtcgtgttgccgcaagtggtaagtttgacggtat
tattgcacttggtgcggtaatccgtggtggtacccctcactttgattttgttgcaggcgaatgtaacaaaggtctggct
caaattgcattagagttcgatctgcccgttgctttcggtgtattgactacagataccattgaacaagctattgagcgtt
caggtactaaagcgggtaacaaaggcggcgaagctgctctaagcttgcttgaaatggtcaacgttctgcaacagcttga
acaacagttgtaa