LOCUS       BBa_K1524100             497 bp    DNA     linear       25-Nov-2024
DEFINITION  H-stem expression vector
ACCESSION   BBa_K1524100
VERSION     BBa_K1524100.1  
FEATURES             Location/Qualifiers
     promoter        1..200
                     /label=Plac (R0010)
     misc_feature    207..242
                     /label=stem up
     misc_feature    243..248
                     /label=NcoI
     misc_feature    249..299
                     /label=dummy anti-sense (for mRFP)
     misc_feature    300..305
                     /label=XhoI
     misc_feature    306..341
                     /label=stem dn
     stem_loop       348..476
                     /label=double terminator (B0015)
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca actagcgccg aggaaggtag cttgtgaggt gtgtggttga
      241 gaccatggtt tgataacgtc ttcggaggaa gccatctagt atttctcctc tttctctagc
      301 tcgagtctca accacacacc tcacaagcta ccttcctcgg cgctagccca ggcatcaaat
      361 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      421 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact
      481 agtagcggcc gctgcag
//