BBa_K1526001
1
BBa_K1526001
Gsh1*: Mutated Glutamate-Cysteine Ligase(Gsh1) from Saccharomyces cerevisiae
2014-10-04T11:00:00Z
2015-05-08T01:10:50Z
http://nar.oxfordjournals.org/content/14/11/4393.full.pdf
Glutamate-Cysteine Ligase(previously known as Gamma glutamylcysteine synthetase) catalyzes the first step in glutathione (GSH) biosynthesis;
L-glutamate + L-cysteine + ATP -> gamma-glutamyl cysteine + ADP + Pi
Expression is induced by oxidants, cadmium, and mercury. Protein abundance increases in response to DNA replication stress. (http://biocyc.org/YEAST/NEW-IMAGE?type=GENE-IN-MAP-IN-PWY&object=YJL101C)
Gsh1* can be used to overproduce cysteine to make gamma-glutamyl cysteine, which is the monomer that forms phytochelatins (n=10-20). In order to do this, glutamate-cysteine ligase is needed to catalyse the reaction. We can overexpress GSH1* using the pYodA(BBa K896008) promoter.
false
false
_1908_
0
21661
9
In stock
false
The original gene(Gsh1/GshA) was mutated to obtain Gsh1*.
false
Ruth Haley
annotation2427852
1
Stop
range2427852
1
1579
1581
annotation2427851
1
Gsh1*
range2427851
1
25
1581
annotation2427850
1
Start Codon
range2427850
1
25
27
annotation2427849
1
BBa_B0034
range2427849
1
7
18
BBa_K1526001_sequence
1
tactagaaagaggagaaatactagatgatccctgatgtgtcacaagccctggcctggctggaaaaacatccgcaggctctgaaaggcattcagcgtggacttgaacgcgaaactctccgcgtaaatgcggacggtacgctggcgacgaccggccatccggaagccttaggctctgcgcttacgcacaaatggatcacgaccgatttcgctgaggccttgctcgaatttattacaccggtggatggcgacattgaacatatgctgacattcatgcgcgacttgcatcggtatacagcgcgtaacatgggagacgaacggatgctcccgctgagcatgccgtgctatatcgcggaaggtcaggacattgaactggcccaatatggtaccagcaacacgggccgctttaagaccctgtatcgcgaagggctgaaaaaccgttacggtgcactgatgcagacaatttccggagtgcactataacttctcactgccaatggcgttctggcaggcgaaatgtggcgacatctctggcgctgacgcgaaagaaaaaatctccgcgggttatttccgcgtgattcgcaactactaccgtttcggctgggttattccgtatctttttggggccagcccggctatttgttccagttttctccaaggcaaaccaacttccctgccgtttgaaaaaacggaatgcggtatgtactacctcccgtacgcaaccagcttacgtctgagcgatctgggctataccaacaaatctcaatcaaatctgggcattacgtttaacgatctttacgaatatgtggcagggctgaaacaggcgattaaaaccccctcggaagaatatgccaaaattggaatcgagaaggacgggaagcgcctacagattaatagtaacgtcctacagatcgaaaacgagctgtacgccccgatccgtcctaaacgtgtttcccgctcgggcgaaagtccgtcagatgccttacttcgtggcggcattgagtatatcgaggttcgctccctggacatcaaccctttcagtcccattggggtggatgaacagcaggtgcgcttcctggacttgtttatggtctggtgtgcgttggcggacgcgcctgaaatgtcttcatcggagctggcgtgtacacgcgtgaactggaatcgcgtgatcctggaaggccgcaaacctggcctgaccctgggtattgggtgcgagacagcccaattcccactccctcaagtgggtaaagacctgttccgtgatttaaaacgcgtcgcgcaaaccctcgactctattaatggtggtgaggcgtatcagaaggtgtgcgacgaacttgtcgcttgttttgataacccggatcttacctttagtgcccgtattttgcgtagcatgatcgataccggaatcggtggcacaggtaaagcgtttgcagaagcatatcggaatttgctgcgcgaggagccgctggaaatcctgcgcgaggaagattttgtagcggaacgtgaagggagcgagcgccgtcagcaggagatggaagctgctgatacggaaccattcgccgtctggttagaaaaacacgcttga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z