BBa_K1526001 1 BBa_K1526001 Gsh1*: Mutated Glutamate-Cysteine Ligase(Gsh1) from Saccharomyces cerevisiae 2014-10-04T11:00:00Z 2015-05-08T01:10:50Z http://nar.oxfordjournals.org/content/14/11/4393.full.pdf Glutamate-Cysteine Ligase(previously known as Gamma glutamylcysteine synthetase) catalyzes the first step in glutathione (GSH) biosynthesis; L-glutamate + L-cysteine + ATP -> gamma-glutamyl cysteine + ADP + Pi Expression is induced by oxidants, cadmium, and mercury. Protein abundance increases in response to DNA replication stress. (http://biocyc.org/YEAST/NEW-IMAGE?type=GENE-IN-MAP-IN-PWY&object=YJL101C) Gsh1* can be used to overproduce cysteine to make gamma-glutamyl cysteine, which is the monomer that forms phytochelatins (n=10-20). In order to do this, glutamate-cysteine ligase is needed to catalyse the reaction. We can overexpress GSH1* using the pYodA(BBa K896008) promoter. false false _1908_ 0 21661 9 In stock false The original gene(Gsh1/GshA) was mutated to obtain Gsh1*. false Ruth Haley annotation2427852 1 Stop range2427852 1 1579 1581 annotation2427849 1 BBa_B0034 range2427849 1 7 18 annotation2427850 1 Start Codon range2427850 1 25 27 annotation2427851 1 Gsh1* range2427851 1 25 1581 BBa_K1526001_sequence 1 tactagaaagaggagaaatactagatgatccctgatgtgtcacaagccctggcctggctggaaaaacatccgcaggctctgaaaggcattcagcgtggacttgaacgcgaaactctccgcgtaaatgcggacggtacgctggcgacgaccggccatccggaagccttaggctctgcgcttacgcacaaatggatcacgaccgatttcgctgaggccttgctcgaatttattacaccggtggatggcgacattgaacatatgctgacattcatgcgcgacttgcatcggtatacagcgcgtaacatgggagacgaacggatgctcccgctgagcatgccgtgctatatcgcggaaggtcaggacattgaactggcccaatatggtaccagcaacacgggccgctttaagaccctgtatcgcgaagggctgaaaaaccgttacggtgcactgatgcagacaatttccggagtgcactataacttctcactgccaatggcgttctggcaggcgaaatgtggcgacatctctggcgctgacgcgaaagaaaaaatctccgcgggttatttccgcgtgattcgcaactactaccgtttcggctgggttattccgtatctttttggggccagcccggctatttgttccagttttctccaaggcaaaccaacttccctgccgtttgaaaaaacggaatgcggtatgtactacctcccgtacgcaaccagcttacgtctgagcgatctgggctataccaacaaatctcaatcaaatctgggcattacgtttaacgatctttacgaatatgtggcagggctgaaacaggcgattaaaaccccctcggaagaatatgccaaaattggaatcgagaaggacgggaagcgcctacagattaatagtaacgtcctacagatcgaaaacgagctgtacgccccgatccgtcctaaacgtgtttcccgctcgggcgaaagtccgtcagatgccttacttcgtggcggcattgagtatatcgaggttcgctccctggacatcaaccctttcagtcccattggggtggatgaacagcaggtgcgcttcctggacttgtttatggtctggtgtgcgttggcggacgcgcctgaaatgtcttcatcggagctggcgtgtacacgcgtgaactggaatcgcgtgatcctggaaggccgcaaacctggcctgaccctgggtattgggtgcgagacagcccaattcccactccctcaagtgggtaaagacctgttccgtgatttaaaacgcgtcgcgcaaaccctcgactctattaatggtggtgaggcgtatcagaaggtgtgcgacgaacttgtcgcttgttttgataacccggatcttacctttagtgcccgtattttgcgtagcatgatcgataccggaatcggtggcacaggtaaagcgtttgcagaagcatatcggaatttgctgcgcgaggagccgctggaaatcctgcgcgaggaagattttgtagcggaacgtgaagggagcgagcgccgtcagcaggagatggaagctgctgatacggaaccattcgccgtctggttagaaaaacacgcttga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z