LOCUS       BBa_K1526005            3147 bp    DNA     linear       23-Nov-2024
DEFINITION  LacRS + Gsh1*
ACCESSION   BBa_K1526005
VERSION     BBa_K1526005.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     RBS             44..54
                     /label=RBS-4\Weaker
     RBS             50..53
                     /label=RBS
     misc_feature    44..54
                     /label=BBa_B0033
     RBS             44..54
                     /label=BBa_B0033
     misc_feature    1150..1188
                     /label=LVA
     CDS             61..1188
                     /label=lacI-LVA
     misc_feature    61..1188
                     /label=BBa_C0012
     misc_feature    1189..1213
                     /label=Help:Barcodes
     CDS             61..1188
                     /label=BBa_C0012
     stem_loop       1233..1276
                     /label=stem_loop
     misc_feature    1222..1301
                     /label=BBa_B0010
     terminator      1222..1301
                     /label=BBa_B0010
     stem_loop       1317..1336
                     /label=T7 TE
     misc_feature    1343..1343
                     /label=stop
     polyA_site      1337..1350
                     /label=polya
     misc_feature    1310..1350
                     /label=BBa_B0012
     terminator      1310..1350
                     /label=BBa_B0012
     misc_feature    44..1350
                     /label=BBa_P0312
     CDS             1359..1446
                     /label=end of LacI coding region (inactive)
     misc_feature    1359..1558
                     /label=BBa_R0010
     misc_feature    1447..1484
                     /label=CAP binding site
     promoter        1495..1500
                     /label=-35
     promoter        1519..1524
                     /label=-10
     misc_feature    1524..1558
                     /label=LacI binding site
     misc_feature    1531..1531
                     /label=start
     promoter        1359..1558
                     /label=BBa_R0010
     promoter        1..1558
                     /label=BBa_K1526009
     RBS             1573..1584
                     /label=BBa_B0034
     misc_feature    1591..1593
                     /label=Start Codon
     CDS             1591..3147
                     /label=Gsh1*
     misc_feature    3145..3147
                     /label=Stop
     misc_feature    1567..3147
                     /label=BBa_K1526001
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagtcacaca ggactactag
       61 atggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat
      121 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa
      181 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg
      241 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg
      301 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg
      361 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa
      421 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa
      481 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac
      541 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg
      601 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt
      661 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa
      721 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc
      781 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt
      841 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa
      901 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg
      961 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag
     1021 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc
     1081 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     1141 agcgggcagg ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc
     1201 tagtgtagat ctctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1261 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     1321 caccttcggg tgggcctttc tgcgtttata tactagagca atacgcaaac cgcctctccc
     1381 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     1441 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     1501 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacata
     1561 ctagagtact agaaagagga gaaatactag atgatccctg atgtgtcaca agccctggcc
     1621 tggctggaaa aacatccgca ggctctgaaa ggcattcagc gtggacttga acgcgaaact
     1681 ctccgcgtaa atgcggacgg tacgctggcg acgaccggcc atccggaagc cttaggctct
     1741 gcgcttacgc acaaatggat cacgaccgat ttcgctgagg ccttgctcga atttattaca
     1801 ccggtggatg gcgacattga acatatgctg acattcatgc gcgacttgca tcggtataca
     1861 gcgcgtaaca tgggagacga acggatgctc ccgctgagca tgccgtgcta tatcgcggaa
     1921 ggtcaggaca ttgaactggc ccaatatggt accagcaaca cgggccgctt taagaccctg
     1981 tatcgcgaag ggctgaaaaa ccgttacggt gcactgatgc agacaatttc cggagtgcac
     2041 tataacttct cactgccaat ggcgttctgg caggcgaaat gtggcgacat ctctggcgct
     2101 gacgcgaaag aaaaaatctc cgcgggttat ttccgcgtga ttcgcaacta ctaccgtttc
     2161 ggctgggtta ttccgtatct ttttggggcc agcccggcta tttgttccag ttttctccaa
     2221 ggcaaaccaa cttccctgcc gtttgaaaaa acggaatgcg gtatgtacta cctcccgtac
     2281 gcaaccagct tacgtctgag cgatctgggc tataccaaca aatctcaatc aaatctgggc
     2341 attacgttta acgatcttta cgaatatgtg gcagggctga aacaggcgat taaaaccccc
     2401 tcggaagaat atgccaaaat tggaatcgag aaggacggga agcgcctaca gattaatagt
     2461 aacgtcctac agatcgaaaa cgagctgtac gccccgatcc gtcctaaacg tgtttcccgc
     2521 tcgggcgaaa gtccgtcaga tgccttactt cgtggcggca ttgagtatat cgaggttcgc
     2581 tccctggaca tcaacccttt cagtcccatt ggggtggatg aacagcaggt gcgcttcctg
     2641 gacttgttta tggtctggtg tgcgttggcg gacgcgcctg aaatgtcttc atcggagctg
     2701 gcgtgtacac gcgtgaactg gaatcgcgtg atcctggaag gccgcaaacc tggcctgacc
     2761 ctgggtattg ggtgcgagac agcccaattc ccactccctc aagtgggtaa agacctgttc
     2821 cgtgatttaa aacgcgtcgc gcaaaccctc gactctatta atggtggtga ggcgtatcag
     2881 aaggtgtgcg acgaacttgt cgcttgtttt gataacccgg atcttacctt tagtgcccgt
     2941 attttgcgta gcatgatcga taccggaatc ggtggcacag gtaaagcgtt tgcagaagca
     3001 tatcggaatt tgctgcgcga ggagccgctg gaaatcctgc gcgaggaaga ttttgtagcg
     3061 gaacgtgaag ggagcgagcg ccgtcagcag gagatggaag ctgctgatac ggaaccattc
     3121 gccgtctggt tagaaaaaca cgcttga
//