LOCUS       BBa_K1526006            2809 bp    DNA     linear       22-Dec-2024
DEFINITION  LacRS + SpPCS
ACCESSION   BBa_K1526006
VERSION     BBa_K1526006.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     RBS             44..54
                     /label=RBS-4\Weaker
     RBS             50..53
                     /label=RBS
     misc_feature    44..54
                     /label=BBa_B0033
     RBS             44..54
                     /label=BBa_B0033
     misc_feature    1150..1188
                     /label=LVA
     CDS             61..1188
                     /label=lacI-LVA
     misc_feature    61..1188
                     /label=BBa_C0012
     misc_feature    1189..1213
                     /label=Help:Barcodes
     CDS             61..1188
                     /label=BBa_C0012
     stem_loop       1233..1276
                     /label=stem_loop
     misc_feature    1222..1301
                     /label=BBa_B0010
     terminator      1222..1301
                     /label=BBa_B0010
     stem_loop       1317..1336
                     /label=T7 TE
     misc_feature    1343..1343
                     /label=stop
     polyA_site      1337..1350
                     /label=polya
     misc_feature    1310..1350
                     /label=BBa_B0012
     terminator      1310..1350
                     /label=BBa_B0012
     misc_feature    44..1350
                     /label=BBa_P0312
     CDS             1359..1446
                     /label=end of LacI coding region (inactive)
     misc_feature    1359..1558
                     /label=BBa_R0010
     misc_feature    1447..1484
                     /label=CAP binding site
     promoter        1495..1500
                     /label=-35
     promoter        1519..1524
                     /label=-10
     misc_feature    1524..1558
                     /label=LacI binding site
     misc_feature    1531..1531
                     /label=start
     promoter        1359..1558
                     /label=BBa_R0010
     promoter        1..1558
                     /label=BBa_K1526009
     RBS             1571..1582
                     /label=BBa_B0034
     misc_feature    1589..1591
                     /label=Start
     misc_feature    2831..2833
                     /label=Stop
     CDS             1589..2833
                     /label=SpPCS
     misc_feature    1565..2809
                     /label=BBa_K1526000
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagtcacaca ggactactag
       61 atggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat
      121 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa
      181 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg
      241 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg
      301 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg
      361 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa
      421 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa
      481 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac
      541 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg
      601 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt
      661 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa
      721 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc
      781 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt
      841 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa
      901 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg
      961 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag
     1021 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc
     1081 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     1141 agcgggcagg ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc
     1201 tagtgtagat ctctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1261 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     1321 caccttcggg tgggcctttc tgcgtttata tactagagca atacgcaaac cgcctctccc
     1381 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     1441 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     1501 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacata
     1561 ctagatgaat attgtaaaac gtgcggtccc ggaactgctg cgcggtatga ccaacgccac
     1621 tccgaatatt gggctgatca aaaataaagt ggtgagtttt gaggccgttg gtcagctcaa
     1681 aaaaagcttt tataaacgtc agcttcccaa acaatgtttg gcttttgatt cttcactggg
     1741 aaaggatgtt ttcctgcgtg cacttcaaga aggccgcatg gaaaattact ttagcctggc
     1801 gcaacaaatg gttacgcaga atgaaccggc gttctgtggc ctgggaacgt tgtgcatgat
     1861 tttaaacagt cttaaagtgg atcctggtcg tctgtggaaa ggcagctggc gttggtatga
     1921 ccagtatatg ctggattgct gccgcagctt atctgatatc gaaaaagatg gggtcaccct
     1981 ggaagagttc agctgtctgg ctaattgcaa tggcttgcgt acaattacaa aatgcgttaa
     2041 agacgtaagt tttgatgagt ttcgtaaaga tgtcattagc tgtagcacca ttgaaaataa
     2101 aatcatggcg attagcttct gccgtaaagt tctgggccag acgggagatg gccattttag
     2161 tccggtgggc ggtttcagcg aatcagataa caaaatcctg attctggatg tggcgcgctt
     2221 taaatacccc tgctattggg tggatctgaa gctgatgtac gaatccatgt tcccgatcga
     2281 taaagcttca ggccagccac gtggatacgt gctgctcgaa ccgatgcata tccccttggg
     2341 cgtgctgacg gtgggattga ataaatattc gtggcgcaat gtgtcgaaac atatcctaca
     2401 gcaagcggcc accgttaaga atgctgacaa tctcgcagaa atcctgttat cgatcaacca
     2461 gtcttccatt ccgctgatcc aggagcgtag caacagcagt aagtcgggcg acttcgaaca
     2521 ttttaaggaa tgcattcgca gtaccaaaac ctatcatctg ttcttaaaac ataccaacac
     2581 gaacgtggaa tacatcacta tggccttctg ggccattttt agtttaccga tgattcaaaa
     2641 agcattaccc aaaggtgttc tggaagaaat ccaatcctta ttgaaagaag tggagatcag
     2701 cgagatcaac acacaactca cggccctgaa aaagcagttg gattcactga cccattgttg
     2761 caaaactgac accggctgtt gcagctcatc ttgttgcaaa aacacttga
//