LOCUS       BBa_K1526008            2629 bp    DNA     linear       26-Jan-2026
DEFINITION  LacRS + CysP
ACCESSION   BBa_K1526008
VERSION     BBa_K1526008.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     RBS             44..54
                     /label=RBS-4\Weaker
     RBS             50..53
                     /label=RBS
     misc_feature    44..54
                     /label=BBa_B0033
     RBS             44..54
                     /label=BBa_B0033
     misc_feature    1150..1188
                     /label=LVA
     CDS             61..1188
                     /label=lacI-LVA
     misc_feature    61..1188
                     /label=BBa_C0012
     misc_feature    1189..1213
                     /label=Help:Barcodes
     CDS             61..1188
                     /label=BBa_C0012
     stem_loop       1233..1276
                     /label=stem_loop
     misc_feature    1222..1301
                     /label=BBa_B0010
     terminator      1222..1301
                     /label=BBa_B0010
     stem_loop       1317..1336
                     /label=T7 TE
     misc_feature    1343..1343
                     /label=stop
     polyA_site      1337..1350
                     /label=polya
     misc_feature    1310..1350
                     /label=BBa_B0012
     terminator      1310..1350
                     /label=BBa_B0012
     misc_feature    44..1350
                     /label=BBa_P0312
     CDS             1359..1446
                     /label=end of LacI coding region (inactive)
     misc_feature    1359..1558
                     /label=BBa_R0010
     misc_feature    1447..1484
                     /label=CAP binding site
     promoter        1495..1500
                     /label=-35
     promoter        1519..1524
                     /label=-10
     misc_feature    1524..1558
                     /label=LacI binding site
     misc_feature    1531..1531
                     /label=start
     promoter        1359..1558
                     /label=BBa_R0010
     promoter        1..1558
                     /label=BBa_K1526009
     RBS             1571..1582
                     /label=BBa_B0034
     misc_feature    1589..1591
                     /label=START
     CDS             1589..2653
                     /label=CysP
     misc_feature    2651..2653
                     /label=STOP
     misc_feature    1565..2629
                     /label=BBa_K1526003
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagtcacaca ggactactag
       61 atggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat
      121 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa
      181 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg
      241 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg
      301 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg
      361 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa
      421 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa
      481 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac
      541 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg
      601 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt
      661 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa
      721 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc
      781 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt
      841 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa
      901 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg
      961 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag
     1021 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc
     1081 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     1141 agcgggcagg ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc
     1201 tagtgtagat ctctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1261 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     1321 caccttcggg tgggcctttc tgcgtttata tactagagca atacgcaaac cgcctctccc
     1381 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     1441 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     1501 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacata
     1561 ctagatggaa ttagccgcta ttttatttag cttgtttttc gcgatgaata ttggtgcaag
     1621 cggcgctgcg gcttcgatgg gggttgctta cggctccgga gccatcaaaa agaaaactta
     1681 cgctttgatc ctatgcgcag tcggtgtctt tgccggggcg gtcattggcg gaggcgaggt
     1741 tgtaaaaaca atcagctccg gcattattcc tgagcagacg attacactga cgattgtttg
     1801 catcattatt ggagctgctg ccctgtcgct ctttactgct aatctgctcg gcattccttt
     1861 gtcgacaagt gaagtaacag tcggcgcggt tgtcggcgtg ggagtggctt ataaagtatt
     1921 gtttgtgaac aatcttctga tcattgtgtc attttgggtt tttgtcccgc tgttcgcttt
     1981 cggcttcacc tatttcgtat ctaagctgtt tcgttatttt aagattgaag taaaatcttc
     2041 taaaaagcaa aaaatcctcg gcattgtttt gttagttgcc ggatttttcg aagcattctc
     2101 tgccggcatg aataacgtgg cgaatgctgt tggcccgtta gtagctgccg gtgtgcttga
     2161 tgtcggaaaa ggaaccttgt atggaggagc ttttgttgct cttggcgcac tgttattagg
     2221 ccggcgagtg ctagaaacaa acgggaagaa aattacgagg ttctccaaag gggaaggcat
     2281 tctcttatcc ggtacaggag cgggactggt gatcatcagt tctgtatttg ggatgccggt
     2341 gcccttggcg caagtcacgt catcgtctat tatcggaata ggcatggcga aaaacggtcc
     2401 gaacgtgttc cataaacaag ttgttcaaac gatgctgaag gtatggatcg tatcgccgtt
     2461 tttatcttta tcgatttctt atctgcttgt atccttgttt ttaaaagcag attattattc
     2521 gatctttatc atggtaagtg tgcttttagc ggcgggcggt gccatcagcc tgacaaaggc
     2581 cattcgtaaa gagagacgct cagtccacga acaaggcggg ggaatctga
//