##gff-version 3
##sequence-region BBa_K1526009 1 1558
BBa_K1526009	.	engineered_region	44	1350	.	+	0	ID=BBa_P0312;Name=BBa_P0312
BBa_K1526009	.	terminator	1310	1350	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0312
BBa_K1526009	.	stem_loop	1317	1336	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1526009	.	stop_codon	1343	1343	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1526009	.	engineered_region	1310	1350	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1526009	.	polyA_site	1337	1350	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1526009	.	ribosome_entry_site	44	54	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_P0312
BBa_K1526009	.	ribosome_entry_site	44	54	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K1526009	.	ribosome_entry_site	50	53	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K1526009	.	engineered_region	44	54	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K1526009	.	terminator	1222	1301	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0312
BBa_K1526009	.	stem_loop	1233	1276	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1526009	.	engineered_region	1222	1301	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1526009	.	CDS	61	1188	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_P0312
BBa_K1526009	.	engineered_tag	1189	1213	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K1526009	.	engineered_region	61	1188	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K1526009	.	tag	1150	1188	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K1526009	.	CDS	61	1188	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K1526009	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100
BBa_K1526009	.	promoter	1359	1558	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K1526009	.	engineered_region	1359	1558	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1526009	.	promoter	1519	1524	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1526009	.	CDS	1359	1446	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1526009	.	non_covalent_binding_site	1447	1484	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1526009	.	start_codon	1531	1531	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1526009	.	non_covalent_binding_site	1524	1558	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1526009	.	promoter	1495	1500	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
>BBa_K1526009
ttgacggctagctcagtcctaggtacagtgctagctactagagtcacacaggactactagatggtgaatgtgaaaccag
taacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgt
ttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactg
gcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcga
ttaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagc
ggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgct
gtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttct
cccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggccc
attaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcg
gaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactg
cgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcgga
tatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggatttt
cgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgc
ccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcatt
aatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaa
taactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggc
ctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgc
gtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtt
tcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacac
tttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca