LOCUS       BBa_K1529020            1633 bp    DNA     linear       22-Dec-2024
DEFINITION  Ptet_rbs_ydeD_rbs_tolC
ACCESSION   BBa_K1529020
VERSION     BBa_K1529020.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    83..86
                     /label=BBa_K1529000
     misc_feature    63..86
                     /label=BBa_K1529001
     misc_feature    1..86
                     /label=BBa_K1529002
     RBS             95..109
                     /label=RBS
     CDS             110..1627
                     /label=TolC
     misc_feature    1628..1633
                     /label=stop
     CDS             95..1633
                     /label=BBa_K554009
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag agatagtact agagattaaa gaggagaaaa tgattgccct
      121 gaacactgcc tcgccacagg ggatgcagat gaagaagctc ctgccgatcc tgatcgggct
      181 aagtctttcc gggttcagct cactttcaca agccgagaat cttatgcaag tatatcagca
      241 ggcacggctg tcgaatccag agcttcgtaa atcagcggcc gatcgagatg ctgcgttcga
      301 gaaaattaat gaggcccggt caccgctgtt accacagctg ggtctcggcg cggattatac
      361 atacagcaac ggctaccgtg atgcgaatgg aataaatagt aacgcaacca gcgcaagctt
      421 acaactgaca cagtcgatct tcgatatgtc gaaatggcgc gcactaacgc ttcaggaaaa
      481 agcagccggg atccaagatg taacttatca gaccgaccag caaacattga ttttgaatac
      541 tgcaaccgct tactttaacg ttctgaatgc catcgacgtg ctgagctaca cccaggcaca
      601 aaaggaagcc atttatcgtc aacttgacca gactacgcag cggtttaacg ttgggttagt
      661 agcaatcacc gatgttcaaa atgcgcgtgc gcagtatgat accgttctgg ccaatgaggt
      721 caccgcacgc aacaatcttg ataatgcggt tgaacagtta cgtcagatta ccgggaacta
      781 ttatcccgag ctggcagcgc tgaatgtgga aaattttaaa actgacaaac ctcaacctgt
      841 caataccctg ctcaaggaag ccgagaaacg caacctgtct ctattgcaag ctcgcctgtc
      901 tcaagacctg gctcgggagc aaatacgcca ggcacaggac ggacatctgc ccacactgga
      961 cctgaccgca agtactggga tatccgacac cagttactct ggatcaaaaa ctcgtggcgc
     1021 cgccggaaca caatacgatg actcaaatat gggccagaac aaggttggcc tgtccttcag
     1081 tttaccgatc taccagggcg ggatggtgaa tagtcaagta aaacaggcgc agtataactt
     1141 tgtcggtgca tcagagcaac tggaatcagc tcaccgtagc gtcgtacaga ctgtccggag
     1201 tagctttaac aacattaacg cgagtatctc atcaatcaat gcttacaagc aagcagtggt
     1261 ttctgcgcag tcttctttgg atgccatgga agcaggctat tcagtgggta ctcgcacgat
     1321 tgttgatgtt ctcgacgcta ccacaactct ctacaacgcg aaacaggaat tagcgaatgc
     1381 ccgttataac tacttaatta accaactgaa tatcaaatct gccctaggta ctctgaatga
     1441 gcaagacttg cttgcgttaa ataacgccct gtccaaacct gtctcaacca atcctgagaa
     1501 cgtagcacct cagacccctg aacagaacgc cattgcggac ggttacgccc ctgacagtcc
     1561 tgcgcctgtg gtccagcaga cgtcagcgcg cactaccact tcaaacgggc ataatccgtt
     1621 ccgcaattaa taa
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