##gff-version 3
##sequence-region BBa_K1529020 1 1633
BBa_K1529020	.	engineered_region	1	86	.	+	0	ID=BBa_K1529002;Name=BBa_K1529002
BBa_K1529020	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K1529002
BBa_K1529020	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1529020	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1529020	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1529020	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1529020	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1529020	.	engineered_region	63	86	.	+	0	ID=BBa_K1529001;Name=BBa_K1529001;Parent=BBa_K1529002
BBa_K1529020	.	engineered_region	83	86	.	+	0	ID=BBa_K1529000;Name=BBa_K1529000;Parent=BBa_K1529001
BBa_K1529020	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1529001
BBa_K1529020	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1529020	.	CDS	95	1633	.	+	0	ID=BBa_K554009;Name=BBa_K554009
BBa_K1529020	.	ribosome_entry_site	95	109	.	+	0	ID=annotation2136926;Name=RBS;Parent=BBa_K554009
BBa_K1529020	.	stop_codon	1628	1633	.	+	0	ID=annotation2136928;Name=stop;Parent=BBa_K554009
BBa_K1529020	.	CDS	110	1627	.	+	0	ID=annotation2136927;Name=TolC;Parent=BBa_K554009
>BBa_K1529020
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gagatagtactagagattaaagaggagaaaatgattgccctgaacactgcctcgccacaggggatgcagatgaagaagc
tcctgccgatcctgatcgggctaagtctttccgggttcagctcactttcacaagccgagaatcttatgcaagtatatca
gcaggcacggctgtcgaatccagagcttcgtaaatcagcggccgatcgagatgctgcgttcgagaaaattaatgaggcc
cggtcaccgctgttaccacagctgggtctcggcgcggattatacatacagcaacggctaccgtgatgcgaatggaataa
atagtaacgcaaccagcgcaagcttacaactgacacagtcgatcttcgatatgtcgaaatggcgcgcactaacgcttca
ggaaaaagcagccgggatccaagatgtaacttatcagaccgaccagcaaacattgattttgaatactgcaaccgcttac
tttaacgttctgaatgccatcgacgtgctgagctacacccaggcacaaaaggaagccatttatcgtcaacttgaccaga
ctacgcagcggtttaacgttgggttagtagcaatcaccgatgttcaaaatgcgcgtgcgcagtatgataccgttctggc
caatgaggtcaccgcacgcaacaatcttgataatgcggttgaacagttacgtcagattaccgggaactattatcccgag
ctggcagcgctgaatgtggaaaattttaaaactgacaaacctcaacctgtcaataccctgctcaaggaagccgagaaac
gcaacctgtctctattgcaagctcgcctgtctcaagacctggctcgggagcaaatacgccaggcacaggacggacatct
gcccacactggacctgaccgcaagtactgggatatccgacaccagttactctggatcaaaaactcgtggcgccgccgga
acacaatacgatgactcaaatatgggccagaacaaggttggcctgtccttcagtttaccgatctaccagggcgggatgg
tgaatagtcaagtaaaacaggcgcagtataactttgtcggtgcatcagagcaactggaatcagctcaccgtagcgtcgt
acagactgtccggagtagctttaacaacattaacgcgagtatctcatcaatcaatgcttacaagcaagcagtggtttct
gcgcagtcttctttggatgccatggaagcaggctattcagtgggtactcgcacgattgttgatgttctcgacgctacca
caactctctacaacgcgaaacaggaattagcgaatgcccgttataactacttaattaaccaactgaatatcaaatctgc
cctaggtactctgaatgagcaagacttgcttgcgttaaataacgccctgtccaaacctgtctcaaccaatcctgagaac
gtagcacctcagacccctgaacagaacgccattgcggacggttacgcccctgacagtcctgcgcctgtggtccagcaga
cgtcagcgcgcactaccacttcaaacgggcataatccgttccgcaattaataa