BBa_R0062 1 lux pR Promoter (luxR & HSL regulated -- lux pR) 2003-01-31T12:00:00Z 2015-05-08T01:14:15Z <em>V. fischeri</em> Released HQ 2013 Promoter activated by LuxR in concert with HSL</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription. false true _1_ 0 24 7 In stock false <P> <P>This promoter is based on the <em>Vibrio fischeri </em>quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <p><img src="<bb_file>Image1.gif</bb_file>" width="614" height="362"><P> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr annotation7070 1 BBa_R0062 range7070 1 1 55 annotation2045 1 LuxR/HSL range2045 1 1 20 annotation2046 1 -35 range2046 1 20 25 annotation2047 1 -10 range2047 1 42 47 annotation2048 1 start range2048 1 53 53 BBa_K1529000 1 BBa_K1529000 YdeD 2014-09-11T11:00:00Z 2015-05-08T01:10:50Z 1 1 false false _1911_ 0 22687 9 Not in stock false 1 false Riku Shinohara BBa_K1529021 1 BBa_K1529021 Plux_rbs_ydeD_rbs_tolC 2014-09-11T11:00:00Z 2015-05-08T01:10:50Z 1 1 false false _1911_ 0 22687 9 Not in stock false 1 false Riku Shinohara component2383206 1 BBa_K554009 component2383202 1 BBa_K1529003 annotation2383206 1 BBa_K554009 range2383206 1 96 1634 annotation2383202 1 BBa_K1529003 range2383202 1 1 87 BBa_K1529003 1 BBa_K1529003 Plux_rbs_ydeD 2014-09-11T11:00:00Z 2015-05-08T01:10:50Z 1 1 false false _1911_ 0 22687 9 Not in stock false 1 false Riku Shinohara component2383131 1 BBa_R0062 component2383139 1 BBa_K1529001 annotation2383131 1 BBa_R0062 range2383131 1 1 55 annotation2383139 1 BBa_K1529001 range2383139 1 64 87 BBa_K1529001 1 BBa_K1529001 rbs_ydeD 2014-09-11T11:00:00Z 2015-05-08T01:10:50Z 1 1 false false _1911_ 0 22687 9 Not in stock false 1 false Riku Shinohara component2382974 1 BBa_K1529000 component2382973 1 BBa_B0034 annotation2382973 1 BBa_B0034 range2382973 1 1 12 annotation2382974 1 BBa_K1529000 range2382974 1 21 24 BBa_K554009 1 BBa_K554009 TolC 2011-09-20T11:00:00Z 2015-05-08T01:12:40Z Synthesized sequence. Released HQ 2013 TolC is ... false false _722_ 0 7971 9 In stock true false UNICAMP EMSE Brazil team annotation2136928 1 stop range2136928 1 1534 1539 annotation2136926 1 RBS range2136926 1 1 15 annotation2136927 1 TolC range2136927 1 16 1533 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1529000_sequence 1 atag BBa_R0062_sequence 1 acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa BBa_B0034_sequence 1 aaagaggagaaa BBa_K1529003_sequence 1 acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagagatag BBa_K1529021_sequence 1 acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagagatagtactagagattaaagaggagaaaatgattgccctgaacactgcctcgccacaggggatgcagatgaagaagctcctgccgatcctgatcgggctaagtctttccgggttcagctcactttcacaagccgagaatcttatgcaagtatatcagcaggcacggctgtcgaatccagagcttcgtaaatcagcggccgatcgagatgctgcgttcgagaaaattaatgaggcccggtcaccgctgttaccacagctgggtctcggcgcggattatacatacagcaacggctaccgtgatgcgaatggaataaatagtaacgcaaccagcgcaagcttacaactgacacagtcgatcttcgatatgtcgaaatggcgcgcactaacgcttcaggaaaaagcagccgggatccaagatgtaacttatcagaccgaccagcaaacattgattttgaatactgcaaccgcttactttaacgttctgaatgccatcgacgtgctgagctacacccaggcacaaaaggaagccatttatcgtcaacttgaccagactacgcagcggtttaacgttgggttagtagcaatcaccgatgttcaaaatgcgcgtgcgcagtatgataccgttctggccaatgaggtcaccgcacgcaacaatcttgataatgcggttgaacagttacgtcagattaccgggaactattatcccgagctggcagcgctgaatgtggaaaattttaaaactgacaaacctcaacctgtcaataccctgctcaaggaagccgagaaacgcaacctgtctctattgcaagctcgcctgtctcaagacctggctcgggagcaaatacgccaggcacaggacggacatctgcccacactggacctgaccgcaagtactgggatatccgacaccagttactctggatcaaaaactcgtggcgccgccggaacacaatacgatgactcaaatatgggccagaacaaggttggcctgtccttcagtttaccgatctaccagggcgggatggtgaatagtcaagtaaaacaggcgcagtataactttgtcggtgcatcagagcaactggaatcagctcaccgtagcgtcgtacagactgtccggagtagctttaacaacattaacgcgagtatctcatcaatcaatgcttacaagcaagcagtggtttctgcgcagtcttctttggatgccatggaagcaggctattcagtgggtactcgcacgattgttgatgttctcgacgctaccacaactctctacaacgcgaaacaggaattagcgaatgcccgttataactacttaattaaccaactgaatatcaaatctgccctaggtactctgaatgagcaagacttgcttgcgttaaataacgccctgtccaaacctgtctcaaccaatcctgagaacgtagcacctcagacccctgaacagaacgccattgcggacggttacgcccctgacagtcctgcgcctgtggtccagcagacgtcagcgcgcactaccacttcaaacgggcataatccgttccgcaattaataa BBa_K1529001_sequence 1 aaagaggagaaatactagagatag BBa_K554009_sequence 1 attaaagaggagaaaatgattgccctgaacactgcctcgccacaggggatgcagatgaagaagctcctgccgatcctgatcgggctaagtctttccgggttcagctcactttcacaagccgagaatcttatgcaagtatatcagcaggcacggctgtcgaatccagagcttcgtaaatcagcggccgatcgagatgctgcgttcgagaaaattaatgaggcccggtcaccgctgttaccacagctgggtctcggcgcggattatacatacagcaacggctaccgtgatgcgaatggaataaatagtaacgcaaccagcgcaagcttacaactgacacagtcgatcttcgatatgtcgaaatggcgcgcactaacgcttcaggaaaaagcagccgggatccaagatgtaacttatcagaccgaccagcaaacattgattttgaatactgcaaccgcttactttaacgttctgaatgccatcgacgtgctgagctacacccaggcacaaaaggaagccatttatcgtcaacttgaccagactacgcagcggtttaacgttgggttagtagcaatcaccgatgttcaaaatgcgcgtgcgcagtatgataccgttctggccaatgaggtcaccgcacgcaacaatcttgataatgcggttgaacagttacgtcagattaccgggaactattatcccgagctggcagcgctgaatgtggaaaattttaaaactgacaaacctcaacctgtcaataccctgctcaaggaagccgagaaacgcaacctgtctctattgcaagctcgcctgtctcaagacctggctcgggagcaaatacgccaggcacaggacggacatctgcccacactggacctgaccgcaagtactgggatatccgacaccagttactctggatcaaaaactcgtggcgccgccggaacacaatacgatgactcaaatatgggccagaacaaggttggcctgtccttcagtttaccgatctaccagggcgggatggtgaatagtcaagtaaaacaggcgcagtataactttgtcggtgcatcagagcaactggaatcagctcaccgtagcgtcgtacagactgtccggagtagctttaacaacattaacgcgagtatctcatcaatcaatgcttacaagcaagcagtggtttctgcgcagtcttctttggatgccatggaagcaggctattcagtgggtactcgcacgattgttgatgttctcgacgctaccacaactctctacaacgcgaaacaggaattagcgaatgcccgttataactacttaattaaccaactgaatatcaaatctgccctaggtactctgaatgagcaagacttgcttgcgttaaataacgccctgtccaaacctgtctcaaccaatcctgagaacgtagcacctcagacccctgaacagaacgccattgcggacggttacgcccctgacagtcctgcgcctgtggtccagcagacgtcagcgcgcactaccacttcaaacgggcataatccgttccgcaattaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z