##gff-version 3
##sequence-region BBa_K1529021 1 1634
BBa_K1529021	.	engineered_region	1	87	.	+	0	ID=BBa_K1529003;Name=BBa_K1529003
BBa_K1529021	.	engineered_region	64	87	.	+	0	ID=BBa_K1529001;Name=BBa_K1529001;Parent=BBa_K1529003
BBa_K1529021	.	engineered_region	84	87	.	+	0	ID=BBa_K1529000;Name=BBa_K1529000;Parent=BBa_K1529001
BBa_K1529021	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1529001
BBa_K1529021	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1529021	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_K1529003
BBa_K1529021	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1529021	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1529021	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1529021	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1529021	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1529021	.	CDS	96	1634	.	+	0	ID=BBa_K554009;Name=BBa_K554009
BBa_K1529021	.	ribosome_entry_site	96	110	.	+	0	ID=annotation2136926;Name=RBS;Parent=BBa_K554009
BBa_K1529021	.	CDS	111	1628	.	+	0	ID=annotation2136927;Name=TolC;Parent=BBa_K554009
BBa_K1529021	.	stop_codon	1629	1634	.	+	0	ID=annotation2136928;Name=stop;Parent=BBa_K554009
>BBa_K1529021
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatact
agagatagtactagagattaaagaggagaaaatgattgccctgaacactgcctcgccacaggggatgcagatgaagaag
ctcctgccgatcctgatcgggctaagtctttccgggttcagctcactttcacaagccgagaatcttatgcaagtatatc
agcaggcacggctgtcgaatccagagcttcgtaaatcagcggccgatcgagatgctgcgttcgagaaaattaatgaggc
ccggtcaccgctgttaccacagctgggtctcggcgcggattatacatacagcaacggctaccgtgatgcgaatggaata
aatagtaacgcaaccagcgcaagcttacaactgacacagtcgatcttcgatatgtcgaaatggcgcgcactaacgcttc
aggaaaaagcagccgggatccaagatgtaacttatcagaccgaccagcaaacattgattttgaatactgcaaccgctta
ctttaacgttctgaatgccatcgacgtgctgagctacacccaggcacaaaaggaagccatttatcgtcaacttgaccag
actacgcagcggtttaacgttgggttagtagcaatcaccgatgttcaaaatgcgcgtgcgcagtatgataccgttctgg
ccaatgaggtcaccgcacgcaacaatcttgataatgcggttgaacagttacgtcagattaccgggaactattatcccga
gctggcagcgctgaatgtggaaaattttaaaactgacaaacctcaacctgtcaataccctgctcaaggaagccgagaaa
cgcaacctgtctctattgcaagctcgcctgtctcaagacctggctcgggagcaaatacgccaggcacaggacggacatc
tgcccacactggacctgaccgcaagtactgggatatccgacaccagttactctggatcaaaaactcgtggcgccgccgg
aacacaatacgatgactcaaatatgggccagaacaaggttggcctgtccttcagtttaccgatctaccagggcgggatg
gtgaatagtcaagtaaaacaggcgcagtataactttgtcggtgcatcagagcaactggaatcagctcaccgtagcgtcg
tacagactgtccggagtagctttaacaacattaacgcgagtatctcatcaatcaatgcttacaagcaagcagtggtttc
tgcgcagtcttctttggatgccatggaagcaggctattcagtgggtactcgcacgattgttgatgttctcgacgctacc
acaactctctacaacgcgaaacaggaattagcgaatgcccgttataactacttaattaaccaactgaatatcaaatctg
ccctaggtactctgaatgagcaagacttgcttgcgttaaataacgccctgtccaaacctgtctcaaccaatcctgagaa
cgtagcacctcagacccctgaacagaacgccattgcggacggttacgcccctgacagtcctgcgcctgtggtccagcag
acgtcagcgcgcactaccacttcaaacgggcataatccgttccgcaattaataa