LOCUS       BBa_K1529080            3056 bp    DNA     linear       13-Jun-2024
DEFINITION  araC_Pbad_M256ICysE_Ptet_luxR_TT
ACCESSION   BBa_K1529080
VERSION     BBa_K1529080.1  
FEATURES             Location/Qualifiers
     promoter        1080..1203
                     /label=pBAD
     promoter        1043..1114
                     /label=PC
     CDS             1..1080
                     /label=araC
     promoter        1..1203
                     /label=BBa_K731201
     RBS             1212..1217
                     /label=RBS
     misc_feature    1218..1225
                     /label=Spacer
     misc_feature    1226..1228
                     /label=Start
     misc_feature    2045..2050
                     /label=Stop
     CDS             1212..2050
                     /label=BBa_K731010
     misc_feature    1..2050
                     /label=BBa_K731030
     misc_feature    2059..2077
                     /label=TetR 1
     misc_feature    2059..2112
                     /label=BBa_R0040
     promoter        2078..2083
                     /label=-35
     misc_feature    2084..2102
                     /label=TetR 2
     promoter        2101..2106
                     /label=-10
     promoter        2059..2112
                     /label=BBa_R0040
     misc_feature    2125..2128
                     /label=conserved
     RBS             2121..2132
                     /label=BBa_B0034
     misc_feature    2139..2140
                     /label=prefix
     misc_feature    2312..2312
                     /label=T
     misc_feature    2630..2630
                     /label=A
     CDS             2139..2888
                     /label=luxR
     misc_feature    2139..2894
                     /label=BBa_C0062
     misc_feature    2895..2919
                     /label=Help:Barcodes
     CDS             2139..2894
                     /label=BBa_C0062
     stem_loop       2939..2982
                     /label=stem_loop
     misc_feature    2928..3007
                     /label=BBa_B0010
     terminator      2928..3007
                     /label=BBa_B0010
     stem_loop       3023..3042
                     /label=T7 TE
     misc_feature    3049..3049
                     /label=stop
     polyA_site      3043..3056
                     /label=polya
     misc_feature    3016..3056
                     /label=BBa_B0012
     terminator      3016..3056
                     /label=BBa_B0012
     terminator      2928..3056
                     /label=BBa_B0015
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac gaaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac tcgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtagtg ctcaaaagca gcttcgcctg
      181 actaatgcgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaacccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cgttaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccaccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgtactaga gaggaggttt ttattatgtc gtgtgaagaa ctggaaattg tctggaacaa
     1261 tattaaagcc gaagccagaa cgctggcgga ctgtgagcca atgctggcca gtttttacca
     1321 cgcgacgcta ctcaagcacg aaaaccttgg cagtgcactg agctacatgc tggcgaacaa
     1381 gctgtcatcg ccaattatgc ctgctattgc tatccgtgaa gtggtggaag aagcctacgc
     1441 cgctgacccg gaaatgatcg cctctgcggc ctgtgatatt caggcggtgc gtacccgcga
     1501 cccggcagtc gataaatact caaccccgtt gttatacctg aagggttttc atgccttgca
     1561 ggcctatcgc atcggtcact ggttgtggaa tcaggggcgt cgcgcactgg caatctttct
     1621 gcaaaaccag gtttctgtga cgttccaggt cgatattcac ccggcagcaa aaattggtcg
     1681 cggtatcatg cttgaccacg cgacaggcat cgtcgttggt gaaacggcgg tgattgaaaa
     1741 cgacgtatcg attctgcaat ctgtgacgct tggcggtacg ggtaaatctg gtggtgaccg
     1801 tcacccgaaa attcgtgaag gtgtgatgat tggcgcgggc gcgaaaatcc tcggcaatat
     1861 tgaagttggg cgcggcgcga agattggcgc aggttccgtg gtgctgcaac cggtgccgcc
     1921 gcataccacc gccgctggcg ttccggctcg tattgtcggt aaaccagaca gcgataagcc
     1981 atcaatggat atcgaccagc atttcaacgg tattaaccat acatttgagt atggggatgg
     2041 gatctaataa tactagagtc cctatcagtg atagagattg acatccctat cagtgataga
     2101 gatactgagc actactagag aaagaggaga aatactagat gaaaaacata aatgccgacg
     2161 acacatacag aataattaat aaaattaaag cttgtagaag caataatgat attaatcaat
     2221 gcttatctga tatgactaaa atggtacatt gtgaatatta tttactcgcg atcatttatc
     2281 ctcattctat ggttaaatct gatatttcaa tcctagataa ttaccctaaa aaatggaggc
     2341 aatattatga tgacgctaat ttaataaaat atgatcctat agtagattat tctaactcca
     2401 atcattcacc aattaattgg aatatatttg aaaacaatgc tgtaaataaa aaatctccaa
     2461 atgtaattaa agaagcgaaa acatcaggtc ttatcactgg gtttagtttc cctattcata
     2521 cggctaacaa tggcttcgga atgcttagtt ttgcacattc agaaaaagac aactatatag
     2581 atagtttatt tttacatgcg tgtatgaaca taccattaat tgttccttct ctagttgata
     2641 attatcgaaa aataaatata gcaaataata aatcaaacaa cgatttaacc aaaagagaaa
     2701 aagaatgttt agcgtgggca tgcgaaggaa aaagctcttg ggatatttca aaaatattag
     2761 gttgcagtga gcgtactgtc actttccatt taaccaatgc gcaaatgaaa ctcaatacaa
     2821 caaaccgctg ccaaagtatt tctaaagcaa ttttaacagg agcaattgat tgcccatact
     2881 ttaaaaatta ataacactga tagtgctagt gtagatcact actagagcca ggcatcaaat
     2941 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     3001 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//